MAGB4_MOUSE
ID MAGB4_MOUSE Reviewed; 556 AA.
AC A2A9R3; B7ZWI9; Q3UNA3;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Melanoma-associated antigen B4 {ECO:0000312|MGI:MGI:2148568};
GN Name=Mageb4 {ECO:0000312|MGI:MGI:2148568};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:EDL29148.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAI50913.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5] {ECO:0000305}
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10706124;
RA Osterlund C., Toehoenen V., Forslund K.O., Nordqvist K.;
RT "Mage-b4, a novel melanoma antigen (MAGE) gene specifically expressed
RT during germ cell differentiation.";
RL Cancer Res. 60:1054-1061(2000).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10706124}.
CC -!- TISSUE SPECIFICITY: Expressed in testis (at protein level).
CC {ECO:0000269|PubMed:10706124}.
CC -!- DEVELOPMENTAL STAGE: Detected between 13.5 and 14.5 dpc in both female
CC and male gonads (at protein level). Increases in the male gonads and
CC intensely expressed before birth (at protein level). In female gonads,
CC remains constant until birth (at protein level). In testis,
CC specifically expressed in premeiotic germ cells (at protein level). In
CC fetal ovary, expressed in both premeiotic germ cells, and germ cells
CC that have gone through the pachytene phase and entered meiotic arrest
CC (at protein level). {ECO:0000269|PubMed:10706124}.
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DR EMBL; AK144342; BAE25842.1; -; mRNA.
DR EMBL; AK144350; BAE25844.1; -; mRNA.
DR EMBL; AL627411; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL954720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466576; EDL29148.1; -; Genomic_DNA.
DR EMBL; BC150912; AAI50913.1; -; mRNA.
DR EMBL; BC172073; AAI72073.1; -; mRNA.
DR CCDS; CCDS30261.1; -.
DR RefSeq; NP_001028664.2; NM_001033492.2.
DR AlphaFoldDB; A2A9R3; -.
DR SMR; A2A9R3; -.
DR STRING; 10090.ENSMUSP00000109602; -.
DR iPTMnet; A2A9R3; -.
DR PhosphoSitePlus; A2A9R3; -.
DR PaxDb; A2A9R3; -.
DR PRIDE; A2A9R3; -.
DR ProteomicsDB; 287296; -.
DR Antibodypedia; 24654; 199 antibodies from 27 providers.
DR Ensembl; ENSMUST00000096378; ENSMUSP00000094107; ENSMUSG00000035427.
DR Ensembl; ENSMUST00000113969; ENSMUSP00000109602; ENSMUSG00000035427.
DR GeneID; 434903; -.
DR KEGG; mmu:434903; -.
DR UCSC; uc009tse.1; mouse.
DR CTD; 4115; -.
DR MGI; MGI:2148568; Mageb4.
DR VEuPathDB; HostDB:ENSMUSG00000035427; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000155485; -.
DR HOGENOM; CLU_489970_0_0_1; -.
DR InParanoid; A2A9R3; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; A2A9R3; -.
DR TreeFam; TF328505; -.
DR BioGRID-ORCS; 434903; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Mageb4; mouse.
DR PRO; PR:A2A9R3; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; A2A9R3; protein.
DR Bgee; ENSMUSG00000035427; Expressed in morula and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR021072; MAGE_N.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR Pfam; PF01454; MAGE; 1.
DR Pfam; PF12440; MAGE_N; 1.
DR SMART; SM01373; MAGE; 1.
DR SMART; SM01392; MAGE_N; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome; Repeat; Tumor antigen.
FT CHAIN 1..556
FT /note="Melanoma-associated antigen B4"
FT /id="PRO_0000437174"
FT DOMAIN 111..310
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REPEAT 334..348
FT /note="1"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 349..363
FT /note="2"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 364..378
FT /note="3"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 379..392
FT /note="4"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 393..407
FT /note="5"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 408..421
FT /note="6"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 422..436
FT /note="7"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 437..451
FT /note="8"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 452..466
FT /note="9"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 467..480
FT /note="10"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 481..495
FT /note="11"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 496..510
FT /note="12"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 511..525
FT /note="13"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 526..539
FT /note="14"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REPEAT 540..554
FT /note="15"
FT /evidence="ECO:0000303|PubMed:10706124"
FT REGION 1..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 315..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..554
FT /note="15 X 15 AA approximate tandem repeats"
FT /evidence="ECO:0000303|PubMed:10706124"
FT COMPBIAS 13..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 325
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 78
FT /note="A -> T (in Ref. 4; AAI72073)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="N -> K (in Ref. 1; BAE25842/BAE25844)"
FT /evidence="ECO:0000305"
FT CONFLICT 444
FT /note="V -> L (in Ref. 1; BAE25842/BAE25844)"
FT /evidence="ECO:0000305"
FT CONFLICT 529..533
FT /note="KVTLG -> NGHTLA (in Ref. 1; BAE25842/BAE25844)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 556 AA; 60922 MW; A2B67EE2398651E0 CRC64;
MPRGQKSKAR AREKRRLVQD EAQELQDAQA KAGEKGKSPS CSNQDSGDAV ASTSTAGFPQ
KSKSQGEAPT TTARKGGACR RSRKSTRGPR EESTSCTRVP RFSENPQNDL LTRKTGMLMQ
YLLCKYKMKQ PASKGEMLKV INRRFKEQLP EILKKASERI QLVFGLEVKE IKPNGGYYTL
VSKLDPSVGN ALTTSLPFPQ NGLLMPLLGV IFLNGNRASE AEIWEFLNVL GIYDGKVHII
FGEPRKLITR DLVKEKYLVY QKEANSDPPS FEFLWGPRAY AETTKMKILE FLAEVNETVP
QAFPTHYEEA LRDQEERAQA EAVGSPGTSA KDKAEAKVTL VDSSCKYQAE SKVTLVDSSC
KDQAESKVTL VDPSCKDNAK SKVTLGSSRK YKAKSKVPLV DSSCKDQAES KVTLVDSCKD
QAESKVTLVD SSCKDQAESK VTLVDSSCKD QAESKVTLVD PSCKDKAKSK VTLGSSHKYK
AKSKVTLVDS SCKDQAESKV TLVDSSCKDQ AESKVTLVDP SCKDNAKSKV TLGSSRKYKA
KSKVPLVDSS GKDKAV
