MAGD1_MOUSE
ID MAGD1_MOUSE Reviewed; 775 AA.
AC Q9QYH6; Q543L6; Q99PB5; Q9CYX1;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Melanoma-associated antigen D1;
DE AltName: Full=Dlxin-1;
DE AltName: Full=MAGE-D1 antigen;
DE AltName: Full=Neurotrophin receptor-interacting MAGE homolog;
GN Name=Maged1; Synonyms=Nrage;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=11084035; DOI=10.1074/jbc.m008590200;
RA Masuda Y., Sasaki A., Shibuya H., Ueno N., Ikeda K., Watanabe K.;
RT "Dlxin-1, a novel protein that binds Dlx5 and regulates its transcriptional
RT function.";
RL J. Biol. Chem. 276:5331-5338(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Auquier P.H., Chomez P.M., De Backer O.R., Bertrand M.J.M.;
RT "Ten new murine members of the MAGE gene family.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Amnion, Embryo, Head, Placenta, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Liver, Lung, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION IN CIRCADIAN RHYTHMS REGULATION, INTERACTION WITH RORA, DISRUPTION
RP PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=20300063; DOI=10.1038/emboj.2010.34;
RA Wang X., Tang J., Xing L., Shi G., Ruan H., Gu X., Liu Z., Wu X., Gao X.,
RA Xu Y.;
RT "Interaction of MAGED1 with nuclear receptors affects circadian clock
RT function.";
RL EMBO J. 29:1389-1400(2010).
CC -!- FUNCTION: Involved in the apoptotic response after nerve growth factor
CC (NGF) binding in neuronal cells. Inhibits cell cycle progression, and
CC facilitates NGFR-mediated apoptosis. May act as a regulator of the
CC function of DLX family members. May enhance ubiquitin ligase activity
CC of RING-type zinc finger-containing E3 ubiquitin-protein ligases.
CC Proposed to act through recruitment and/or stabilization of the Ubl-
CC conjugating enzyme (E2) at the E3:substrate complex. Plays a role in
CC the circadian rhythm regulation. May act as RORA coregulator,
CC modulating the expression of core clock genes such as ARNTL/BMAL1 and
CC NFIL3, induced, or NR1D1, repressed. {ECO:0000269|PubMed:20300063}.
CC -!- SUBUNIT: Interacts with DLX5, DLX7 and MSX2 and forms homomultimers.
CC Interacts with UNC5A. Interacts with TRIM28 and PJA1. Interacts with
CC NGFR/p75NTR and RORA. {ECO:0000269|PubMed:20300063}.
CC -!- INTERACTION:
CC Q9QYH6; P70396: Dlx5; NbExp=2; IntAct=EBI-1801274, EBI-1801294;
CC Q9QYH6; Q03358: Msx2; NbExp=5; IntAct=EBI-1801274, EBI-1801354;
CC Q9QYH6; P25233: Ndn; NbExp=7; IntAct=EBI-1801274, EBI-1801080;
CC Q9QYH6; O55176-2: Pja1; NbExp=2; IntAct=EBI-1801274, EBI-1801670;
CC Q9QYH6; P51448: Rora; NbExp=5; IntAct=EBI-1801274, EBI-1169722;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20300063}. Cytoplasm
CC {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}. Note=Expression shifts from the cytoplasm to the plasma
CC membrane upon stimulation with NGF. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in many adult tissues,
CC except for the spleen. Expressed in osteoblastic and chondrogenic cell
CC lines and also during embryonic development.
CC -!- DISRUPTION PHENOTYPE: Animals show shortened circadian period and
CC reduced total activity. {ECO:0000269|PubMed:20300063}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB029448; BAA87959.1; -; mRNA.
DR EMBL; AF319975; AAK01203.1; -; mRNA.
DR EMBL; AK013231; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK017275; BAB30666.1; -; mRNA.
DR EMBL; AK049660; BAC33863.1; -; mRNA.
DR EMBL; AK132248; BAE21057.1; -; mRNA.
DR EMBL; AK145872; BAE26714.1; -; mRNA.
DR EMBL; AK146908; BAE27521.1; -; mRNA.
DR CCDS; CCDS30280.1; -.
DR RefSeq; NP_062765.1; NM_019791.2.
DR AlphaFoldDB; Q9QYH6; -.
DR SMR; Q9QYH6; -.
DR BioGRID; 220497; 17.
DR IntAct; Q9QYH6; 14.
DR MINT; Q9QYH6; -.
DR STRING; 10090.ENSMUSP00000026142; -.
DR iPTMnet; Q9QYH6; -.
DR PhosphoSitePlus; Q9QYH6; -.
DR jPOST; Q9QYH6; -.
DR MaxQB; Q9QYH6; -.
DR PaxDb; Q9QYH6; -.
DR PRIDE; Q9QYH6; -.
DR ProteomicsDB; 292076; -.
DR Antibodypedia; 4194; 361 antibodies from 36 providers.
DR DNASU; 94275; -.
DR Ensembl; ENSMUST00000026142; ENSMUSP00000026142; ENSMUSG00000025151.
DR GeneID; 94275; -.
DR KEGG; mmu:94275; -.
DR UCSC; uc009ttn.1; mouse.
DR CTD; 9500; -.
DR MGI; MGI:1930187; Maged1.
DR VEuPathDB; HostDB:ENSMUSG00000025151; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000162070; -.
DR HOGENOM; CLU_394113_0_0_1; -.
DR InParanoid; Q9QYH6; -.
DR OMA; AVCHPLP; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; Q9QYH6; -.
DR TreeFam; TF352132; -.
DR BioGRID-ORCS; 94275; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Maged1; mouse.
DR PRO; PR:Q9QYH6; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q9QYH6; protein.
DR Bgee; ENSMUSG00000025151; Expressed in placenta labyrinth and 280 other tissues.
DR Genevisible; Q9QYH6; MM.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:MGI.
DR GO; GO:0032922; P:circadian regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; ISO:MGI.
DR GO; GO:1900181; P:negative regulation of protein localization to nucleus; IDA:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:2001235; P:positive regulation of apoptotic signaling pathway; IMP:MGI.
DR GO; GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; IMP:UniProtKB.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0034504; P:protein localization to nucleus; IDA:MGI.
DR GO; GO:0042752; P:regulation of circadian rhythm; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR030083; MAGED1.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR PANTHER; PTHR11736:SF28; PTHR11736:SF28; 1.
DR Pfam; PF01454; MAGE; 1.
DR SMART; SM01373; MAGE; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Biological rhythms; Cell membrane; Cytoplasm; Membrane; Nucleus;
KW Reference proteome; Repeat; Tumor antigen; Ubl conjugation pathway.
FT CHAIN 1..775
FT /note="Melanoma-associated antigen D1"
FT /id="PRO_0000156724"
FT REPEAT 292..297
FT /note="1"
FT REPEAT 298..303
FT /note="2"
FT REPEAT 304..309
FT /note="3"
FT REPEAT 329..334
FT /note="4"
FT REPEAT 335..340
FT /note="5"
FT REPEAT 341..346
FT /note="6"
FT REPEAT 347..352
FT /note="7"
FT REPEAT 353..358
FT /note="8"
FT REPEAT 359..364
FT /note="9"
FT REPEAT 365..370
FT /note="10"
FT REPEAT 371..376
FT /note="11"
FT REPEAT 377..382
FT /note="12"
FT REPEAT 383..388
FT /note="13"
FT REPEAT 389..394
FT /note="14"
FT REPEAT 395..400
FT /note="15"
FT REPEAT 401..406
FT /note="16"
FT REPEAT 407..412
FT /note="17"
FT REPEAT 413..418
FT /note="18"
FT REPEAT 419..424
FT /note="19"
FT REPEAT 425..429
FT /note="20; approximate"
FT REPEAT 430..435
FT /note="21"
FT REPEAT 436..441
FT /note="22"
FT DOMAIN 468..666
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 37..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..441
FT /note="22 X 6 AA tandem repeats of W-[PQ]-X-P-X-X"
FT REGION 374..409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..237
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 357..362
FT /note="Missing (in Ref. 2; AAK01203)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 775 AA; 85670 MW; 224B82470816835A CRC64;
MAQKPDGGAG LRGFQAEASV EDSALLVQTL MEAIQISEAP PTSQATAAAS GPNASPQSSQ
PPTANEKADT EVSAAAARPK TGFKAQNATT KGPNDYSQAR NAKEMPKNQS KAAFKSQNGT
PKGPHAASDF SQAAPTGKSA KKSEMAFKGQ NSTKAGPGTT YNFPQSPSAN EMTNNQPKTA
KAWNDTTKVP GADAQTQNVN QAKMADVGTS AGISEADGAA AQTSADGSQT QNVESRTIIR
GKRTRKVNNL NVEENNSGDQ RRASLASGNW RSAPVPVTTQ QNPPGAPPNV VWQTPLAWQN
PSGWQNQTAR QTPPAARQSP PARQTPSAWQ NPVAWQNPVI WPNPVIWQNP VIWPNPIVWP
GPIVWPNPMA WQSTPGWQSP PSWQAPPSWQ SPQDWQGPPD WQVPPDWSMP PDWSFPSDWP
FPPDWIPADW PIPPDWQNLR PSPNLRSSSN SRASQNQGPP QPRDVALLQE RANKLVKYLM
LKDYTKVPIK RSEMLRDIIR EYTDVYPEII ERACFVLEKK FGIQLKEIDK EEHLYILIST
PESLAGILGT TKDTPKLGLL LVILGIIFMN GNRATEAVLW EALRKMGLRP GVRHPLLGDL
RKLLTYEFVK QKYLDYRRVP NSNPPEYEFL WGLRSYHETS KMKVLRFIAE VQKRDPRDWT
AQFMEAADEA LDALDAAAAE AEARAEARNR MGIGDEAVSG PWSWDDIEFE LLTWDEEGDF
GDPWSRIPFT FWARYHQNAR SRFPQAFTGP IIGPSGTATA NFAANFGAIG FFWVE
