MAGD1_RAT
ID MAGD1_RAT Reviewed; 775 AA.
AC Q9ES73; Q66HP3; Q9JHZ6; Q9QX92;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Melanoma-associated antigen D1;
DE AltName: Full=MAGE-D1 antigen;
DE AltName: Full=Neurotrophin receptor-interacting MAGE homolog;
DE AltName: Full=Sertoli cell necdin-related gene protein 1;
DE Short=SNERG-1;
GN Name=Maged1; Synonyms=Nrage;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS, AND INTERACTION WITH
RP NGFR.
RC STRAIN=Sprague-Dawley; TISSUE=Neural crest;
RX PubMed=10985348; DOI=10.1016/s0896-6273(00)00036-2;
RA Salehi A.H., Roux P.P., Kubu C.J., Zeindler C., Bhakar A., Tannis L.-L.,
RA Verdi J.M., Barker P.A.;
RT "NRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor
RT and facilitates nerve growth factor dependent apoptosis.";
RL Neuron 27:279-288(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 206-775.
RC TISSUE=Testis;
RX PubMed=11014239; DOI=10.1210/endo.141.10.7705;
RA Hennuy B., Reiter E., Cornet A., Bruyninx M., Daukandt M., Houssa P.,
RA N'Guyen V.-H., Closset J., Hennen G.;
RT "A novel messenger ribonucleic acid homologous to human MAGE-D is strongly
RT expressed in rat Sertoli cells and weakly in Leydig cells and is regulated
RT by follitropin, lutropin, and prolactin.";
RL Endocrinology 141:3821-3831(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 178-775.
RA Zhang C., He F.;
RT "Rattus norvegicus mRNA for SNERG-1 protein, partial CDS.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH UNC5A.
RX PubMed=12598531; DOI=10.1074/jbc.m300415200;
RA Williams M.E., Strickland P., Watanabe K., Hinck L.;
RT "UNC5H1 induces apoptosis via its juxtamembrane region through an
RT interaction with NRAGE.";
RL J. Biol. Chem. 278:17483-17490(2003).
CC -!- FUNCTION: Involved in the apoptotic response after nerve growth factor
CC (NGF) binding in neuronal cells. Inhibits cell cycle progression, and
CC facilitates NGFR-mediated apoptosis. May act as a regulator of the
CC function of DLX family members. May enhance ubiquitin ligase activity
CC of RING-type zinc finger-containing E3 ubiquitin-protein ligases.
CC Proposed to act through recruitment and/or stabilization of the Ubl-
CC conjugating enzyme (E2) at the E3:substrate complex. Plays a role in
CC the circadian rhythm regulation. May act as RORA co-regulator,
CC modulating the expression of core clock genes such as ARNTL/BMAL1 and
CC NFIL3, induced, or NR1D1, repressed. {ECO:0000269|PubMed:10985348}.
CC -!- SUBUNIT: Interacts with DLX5, DLX7 and MSX2 and forms homomultimers.
CC Interacts with UNC5A. Interacts with TRIM28 and PJA1. Interacts with
CC NGFR/p75NTR and RORA. {ECO:0000269|PubMed:10985348,
CC ECO:0000269|PubMed:12598531}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane
CC protein. Nucleus {ECO:0000250}. Note=Expression shifts from the
CC cytoplasm to the plasma membrane upon stimulation with NGF.
CC -!- TISSUE SPECIFICITY: Ubiquitous and in the seminiferous tubules
CC expressed in Sertoli cells but not in germ cells. Expression decreases
CC in all tissues with increased age and is detectable only in brain
CC cortex and lung.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels throughout the embryo and
CC is enriched in the developing brain and spinal cord.
CC -!- INDUCTION: Follitropin decreased expression while lutropin and
CC prolactin stimulated expression.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB65381.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF217964; AAG09705.1; -; mRNA.
DR EMBL; BC081756; AAH81756.1; -; mRNA.
DR EMBL; AJ133038; CAB65381.1; ALT_FRAME; mRNA.
DR EMBL; AF274043; AAF75283.1; -; mRNA.
DR RefSeq; NP_445861.1; NM_053409.2.
DR AlphaFoldDB; Q9ES73; -.
DR SMR; Q9ES73; -.
DR BioGRID; 249969; 2.
DR STRING; 10116.ENSRNOP00000009870; -.
DR iPTMnet; Q9ES73; -.
DR PhosphoSitePlus; Q9ES73; -.
DR jPOST; Q9ES73; -.
DR PaxDb; Q9ES73; -.
DR PRIDE; Q9ES73; -.
DR Ensembl; ENSRNOT00000009870; ENSRNOP00000009870; ENSRNOG00000006756.
DR GeneID; 84469; -.
DR KEGG; rno:84469; -.
DR CTD; 9500; -.
DR RGD; 70898; Maged1.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000162070; -.
DR HOGENOM; CLU_394113_0_0_1; -.
DR InParanoid; Q9ES73; -.
DR OMA; AVCHPLP; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; Q9ES73; -.
DR PRO; PR:Q9ES73; -.
DR Proteomes; UP000002494; Chromosome X.
DR Bgee; ENSRNOG00000006756; Expressed in ovary and 20 other tissues.
DR ExpressionAtlas; Q9ES73; baseline and differential.
DR Genevisible; Q9ES73; RN.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0004705; F:JUN kinase activity; TAS:Reactome.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:RGD.
DR GO; GO:0032922; P:circadian regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; ISO:RGD.
DR GO; GO:1900181; P:negative regulation of protein localization to nucleus; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:2001235; P:positive regulation of apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; ISO:RGD.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0034504; P:protein localization to nucleus; IEA:Ensembl.
DR GO; GO:0042752; P:regulation of circadian rhythm; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR030083; MAGED1.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR PANTHER; PTHR11736:SF28; PTHR11736:SF28; 1.
DR Pfam; PF01454; MAGE; 1.
DR SMART; SM01373; MAGE; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Biological rhythms; Cell membrane; Cytoplasm; Membrane; Nucleus;
KW Reference proteome; Repeat; Tumor antigen; Ubl conjugation pathway.
FT CHAIN 1..775
FT /note="Melanoma-associated antigen D1"
FT /id="PRO_0000156726"
FT REPEAT 293..298
FT /note="1"
FT REPEAT 299..304
FT /note="2"
FT REPEAT 305..310
FT /note="3"
FT REPEAT 329..334
FT /note="4"
FT REPEAT 335..340
FT /note="5"
FT REPEAT 341..346
FT /note="6"
FT REPEAT 347..352
FT /note="7"
FT REPEAT 353..358
FT /note="8"
FT REPEAT 359..364
FT /note="9"
FT REPEAT 365..370
FT /note="10"
FT REPEAT 371..376
FT /note="11"
FT REPEAT 377..382
FT /note="12"
FT REPEAT 383..388
FT /note="13"
FT REPEAT 389..394
FT /note="14"
FT REPEAT 395..400
FT /note="15"
FT REPEAT 401..406
FT /note="16"
FT REPEAT 407..412
FT /note="17"
FT REPEAT 413..418
FT /note="18"
FT REPEAT 419..424
FT /note="19"
FT REPEAT 425..429
FT /note="20; approximate"
FT REPEAT 430..435
FT /note="21"
FT REPEAT 436..441
FT /note="22"
FT DOMAIN 468..666
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 37..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..441
FT /note="22 X 6 AA tandem repeats of W-[PQ]-X-P-X-X"
FT REGION 374..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..239
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 178
FT /note="Q -> R (in Ref. 4; AAF75283)"
FT /evidence="ECO:0000305"
FT CONFLICT 425..427
FT /note="WIP -> CIL (in Ref. 3; CAB65381 and 4; AAF75283)"
FT /evidence="ECO:0000305"
FT CONFLICT 693..699
FT /note="IGDEAVS -> LETELCL (in Ref. 3; CAB65381 and 4;
FT AAF75283)"
FT /evidence="ECO:0000305"
FT CONFLICT 724..725
FT /note="WS -> GP (in Ref. 4; AAF75283)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 775 AA; 85798 MW; F8BF3B170B8A3742 CRC64;
MAQKPDGGAG LRGFQAEASV EDSALLVQTL MEAIQISEAP PTSQATAAAS GPNASPQSSQ
PPTANEKADT EVSAAAARPK TGFKAQNTTT KGPNDYSQAR NAKEMPKNQP KVAFKSQNAT
SKGPHAASDF SHAASTGKSA AKKSEMAFKG QNTTTKAGPS ATYNFTQSPS ANEMTNNQPK
TAKAWNDTTK IPGADAQTQN VNQAKMADVG TSAGISETDG AAAQTSADGS QAQNVESRTI
IRGKRTRKIN NLNVEENSNG DQRRASLASG NWRSAPVPVT TQNPPGAPPN VLWQTPLAWQ
NPSGWQNQTA RQTPPARQSP PARQTPSAWQ NPVAWQNPVI WPNPVIWQNP VIWPNPIVWP
GPIVWPNPMA WQSTPGWQSP PSWQAPPSWQ SPQDWQGPPD WQLPPDWSMP PDWSFPSDWP
FPPDWIPADW PIPPDWQNLR PSPNLRSSPN SRASQNQGPP QPRDVALLQE RANKLVKYLM
LKDYTKVPIK RSEMLRDIIR EYTDVYPEII ERACFVLEKK FGIQLKEIDK EEHLYILIST
PESLAGILGT TKDTPKLGLL LVILGIIFMN GNRATEAVLW EALRKMGLRP GVRHPLLGDL
RKLLTYEFVK QKYLDYRRVP NSNPPEYEFL WGLRSYHETS KMKVLRFIAE VQKRDPRDWT
AQFMEAADEA LDALDAAAAE AEARAEARNR MGIGDEAVSG PWSWDDIEFE LLTWDEEGDF
GDPWSRIPFT FWARYHQNAR SRFPQAFTGP IIGPSGTATA NFAANFGAIG FFWVE
