MAGT1_DANRE
ID MAGT1_DANRE Reviewed; 328 AA.
AC Q7ZV50;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Magnesium transporter protein 1 {ECO:0000250|UniProtKB:Q9H0U3};
DE Short=MagT1;
DE AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1;
DE Short=Oligosaccharyl transferase subunit MAGT1;
DE Flags: Precursor;
GN Name=magt1 {ECO:0000250|UniProtKB:Q9H0U3}; ORFNames=zgc:56218;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TOPOLOGY.
RX PubMed=19717468; DOI=10.1073/pnas.0908332106;
RA Zhou H., Clapham D.E.;
RT "Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and
RT vertebrate embryonic development.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:15750-15755(2009).
CC -!- FUNCTION: Cell surface magnesium transporter.
CC {ECO:0000269|PubMed:19717468}.
CC -!- FUNCTION: Accessory component of the STT3B-containing form of the N-
CC oligosaccharyl transferase (OST) complex which catalyzes the transfer
CC of a high mannose oligosaccharide from a lipid-linked oligosaccharide
CC donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif
CC in nascent polypeptide chains. May be involved in substrate-specific N-
CC glycosylation. {ECO:0000250|UniProtKB:Q9H0U3}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000250|UniProtKB:Q9H0U3}.
CC -!- SUBUNIT: Accessory component of the STT3B-containing form of the
CC oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:Q9H0U3}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9H0U3};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9H0U3}. Endoplasmic
CC reticulum {ECO:0000250|UniProtKB:Q9H0U3}. Endoplasmic reticulum
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Early developmental arrest.
CC {ECO:0000269|PubMed:19717468}.
CC -!- SIMILARITY: Belongs to the OST3/OST6 family. {ECO:0000305}.
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DR EMBL; BC046002; AAH46002.1; -; mRNA.
DR RefSeq; NP_955994.1; NM_199700.1.
DR AlphaFoldDB; Q7ZV50; -.
DR SMR; Q7ZV50; -.
DR STRING; 7955.ENSDARP00000075309; -.
DR TCDB; 1.A.76.1.3; the magnesium transporter1 (magt1) family.
DR PaxDb; Q7ZV50; -.
DR Ensembl; ENSDART00000080864; ENSDARP00000075309; ENSDARG00000058062.
DR GeneID; 324944; -.
DR KEGG; dre:324944; -.
DR CTD; 84061; -.
DR ZFIN; ZDB-GENE-030131-3667; magt1.
DR eggNOG; KOG2603; Eukaryota.
DR GeneTree; ENSGT00390000012030; -.
DR HOGENOM; CLU_052855_0_0_1; -.
DR InParanoid; Q7ZV50; -.
DR OMA; IFQQMNL; -.
DR OrthoDB; 1460433at2759; -.
DR PhylomeDB; Q7ZV50; -.
DR TreeFam; TF314850; -.
DR Reactome; R-DRE-5223345; Miscellaneous transport and binding events.
DR Reactome; R-DRE-6798695; Neutrophil degranulation.
DR UniPathway; UPA00378; -.
DR PRO; PR:Q7ZV50; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 14.
DR Bgee; ENSDARG00000058062; Expressed in granulocyte and 27 other tissues.
DR ExpressionAtlas; Q7ZV50; baseline.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; IGI:ZFIN.
DR GO; GO:0006487; P:protein N-linked glycosylation; IEA:InterPro.
DR InterPro; IPR006844; Mg_transporter-1.
DR InterPro; IPR021149; OligosaccharylTrfase_OST3/OST6.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR12692; PTHR12692; 1.
DR PANTHER; PTHR12692:SF2; PTHR12692:SF2; 1.
DR Pfam; PF04756; OST3_OST6; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Endoplasmic reticulum; Glycoprotein;
KW Magnesium; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..328
FT /note="Magnesium transporter protein 1"
FT /id="PRO_0000246061"
FT TOPO_DOM 23..177
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..202
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..257
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 315..328
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 40..168
FT /note="Thioredoxin"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 80..83
FT /note="Redox-active"
FT /evidence="ECO:0000250"
SQ SEQUENCE 328 AA; 37422 MW; A68197BF2D7D6EE6 CRC64;
MLHKLLIVVF LVVCLHDMRL NGQKKKETLL SEKVSQMMEW VSKRAVVRLN GEKFKRLVRA
HPRNYSVIVM FTALQPQRQC GVCRQADEEY QILANSWRYS SAFTNRIFFA MVDFDEGSDV
FQMLNMNSAP TFINFPAKGK PKRADTYELQ VRGFAAEQLA RWVADRTDVH IRVIRPPNYA
GPLMLGLLLA FIGSLAYLRR NNLEFLFNKN VWAFSALCFV LIMTSGQMWN HIRGPPYAHK
NPNTGQVSYI HGSSQAQFVA ETHIVLLFNA AVTIGMVLLH EAATSGLDIV KRKIMCVAGI
GLVVLFFSWL LSVFRAKYHG YPYSFLFG
