MAK16_MOUSE
ID MAK16_MOUSE Reviewed; 296 AA.
AC Q8BGS0; Q921E0; Q9D5G6;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein MAK16 homolog;
DE AltName: Full=Protein RBM13;
GN Name=Mak16; Synonyms=Rbm13;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-197 (ISOFORM 2), AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BGS0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BGS0-2; Sequence=VSP_014840;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MAK16 family. {ECO:0000305}.
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DR EMBL; AK015369; BAB29815.1; -; mRNA.
DR EMBL; AK075982; BAC36094.1; -; mRNA.
DR EMBL; AK075983; BAC36095.1; -; mRNA.
DR EMBL; BC013079; AAH13079.1; -; mRNA.
DR CCDS; CCDS22222.1; -. [Q8BGS0-1]
DR RefSeq; NP_080729.1; NM_026453.3. [Q8BGS0-1]
DR AlphaFoldDB; Q8BGS0; -.
DR SMR; Q8BGS0; -.
DR BioGRID; 212534; 3.
DR STRING; 10090.ENSMUSP00000033983; -.
DR iPTMnet; Q8BGS0; -.
DR PhosphoSitePlus; Q8BGS0; -.
DR SwissPalm; Q8BGS0; -.
DR EPD; Q8BGS0; -.
DR jPOST; Q8BGS0; -.
DR MaxQB; Q8BGS0; -.
DR PaxDb; Q8BGS0; -.
DR PeptideAtlas; Q8BGS0; -.
DR PRIDE; Q8BGS0; -.
DR ProteomicsDB; 292006; -. [Q8BGS0-1]
DR ProteomicsDB; 292007; -. [Q8BGS0-2]
DR Antibodypedia; 23360; 57 antibodies from 17 providers.
DR DNASU; 67920; -.
DR Ensembl; ENSMUST00000033983; ENSMUSP00000033983; ENSMUSG00000031578. [Q8BGS0-1]
DR GeneID; 67920; -.
DR KEGG; mmu:67920; -.
DR UCSC; uc009lji.1; mouse. [Q8BGS0-1]
DR CTD; 84549; -.
DR MGI; MGI:1915170; Mak16.
DR VEuPathDB; HostDB:ENSMUSG00000031578; -.
DR eggNOG; KOG3064; Eukaryota.
DR GeneTree; ENSGT00390000012859; -.
DR HOGENOM; CLU_050888_2_0_1; -.
DR InParanoid; Q8BGS0; -.
DR OMA; GTYRDIY; -.
DR OrthoDB; 1394336at2759; -.
DR PhylomeDB; Q8BGS0; -.
DR TreeFam; TF105759; -.
DR BioGRID-ORCS; 67920; 25 hits in 72 CRISPR screens.
DR ChiTaRS; Mak16; mouse.
DR PRO; PR:Q8BGS0; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8BGS0; protein.
DR Bgee; ENSMUSG00000031578; Expressed in embryonic post-anal tail and 280 other tissues.
DR Genevisible; Q8BGS0; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IBA:GO_Central.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR InterPro; IPR029004; L28e/Mak16.
DR InterPro; IPR006958; Mak16.
DR Pfam; PF04874; Mak16; 1.
DR Pfam; PF01778; Ribosomal_L28e; 1.
DR PIRSF; PIRSF003352; MAK16; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Isopeptide bond; Nucleus;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..296
FT /note="Protein MAK16 homolog"
FT /id="PRO_0000203795"
FT REGION 190..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 196..252
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BXY0"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 228
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BXY0"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BXY0"
FT CROSSLNK 55
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BXY0"
FT CROSSLNK 151
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BXY0"
FT VAR_SEQ 200..205
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014840"
FT CONFLICT 31
FT /note="N -> S (in Ref. 1; BAB29815)"
FT /evidence="ECO:0000305"
FT CONFLICT 254
FT /note="D -> E (in Ref. 2; AAH13079)"
FT /evidence="ECO:0000305"
FT MOD_RES Q8BGS0-2:197
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 296 AA; 35185 MW; 777A4DC9F453EA53 CRC64;
MQSDDVIWNT LGNKQFCSFK IRTKTQGFCR NEYSLTGLCN RSSCPLANSQ YATIKEEKGQ
CYLYMKVIER AAFPRRLWER VRLSKNYEKA LEQIDENLIY WPRFIRHKCK QRFTKITQYL
IRIRKLTLKR QRKLVPLSKK VERREKRREE KALIAAQLDN AIEKELLERL KQDTYGDIYN
FPIHAFDKAL EKQEAESDSE DEEEEEDEDE EEDVGKREFV EDEEVEESDL SDFEDMDKLN
TDSEEDQDDE SSNDEEAHKA KHKGKAPLKG PLRKKRAYVE IEYEQETEPM AKVKAT
