MAK16_YEAST
ID MAK16_YEAST Reviewed; 306 AA.
AC P10962; D6VPJ3;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Protein MAK16;
DE AltName: Full=Maintenance of killer protein 16;
GN Name=MAK16; OrderedLocusNames=YAL025C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=3045810; DOI=10.1073/pnas.85.16.6007;
RA Wickner R.B.;
RT "Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces
RT cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:6007-6011(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 250.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Its role might be as part of the apparatus concerned with the
CC nuclear events of the cell cycle. {ECO:0000269|PubMed:3045810}.
CC -!- INTERACTION:
CC P10962; Q06218: DBP9; NbExp=4; IntAct=EBI-10937, EBI-5640;
CC P10962; P39744: NOC2; NbExp=3; IntAct=EBI-10937, EBI-29259;
CC P10962; P38805: RPF1; NbExp=3; IntAct=EBI-10937, EBI-24614;
CC P10962; P34241: URB1; NbExp=3; IntAct=EBI-10937, EBI-26595;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the MAK16 family. {ECO:0000305}.
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DR EMBL; J03852; AAA34752.1; -; Genomic_DNA.
DR EMBL; U12980; AAC05007.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA06963.2; -; Genomic_DNA.
DR PIR; A35588; BVBYK6.
DR RefSeq; NP_009377.2; NM_001178170.2.
DR PDB; 5Z3G; EM; 3.65 A; Z=1-306.
DR PDB; 6C0F; EM; 3.70 A; D=1-306.
DR PDB; 6CB1; EM; 4.60 A; D=1-306.
DR PDB; 6EM1; EM; 3.60 A; 3=1-306.
DR PDB; 6EM3; EM; 3.20 A; 3=1-306.
DR PDB; 6EM4; EM; 4.10 A; 3=1-306.
DR PDB; 6EM5; EM; 4.30 A; 3=1-306.
DR PDB; 7OHS; EM; 4.38 A; 3=1-306.
DR PDB; 7OHW; EM; 3.50 A; 3=1-306.
DR PDB; 7OHX; EM; 3.30 A; 3=1-306.
DR PDBsum; 5Z3G; -.
DR PDBsum; 6C0F; -.
DR PDBsum; 6CB1; -.
DR PDBsum; 6EM1; -.
DR PDBsum; 6EM3; -.
DR PDBsum; 6EM4; -.
DR PDBsum; 6EM5; -.
DR PDBsum; 7OHS; -.
DR PDBsum; 7OHW; -.
DR PDBsum; 7OHX; -.
DR AlphaFoldDB; P10962; -.
DR SMR; P10962; -.
DR BioGRID; 31741; 355.
DR DIP; DIP-6521N; -.
DR IntAct; P10962; 13.
DR MINT; P10962; -.
DR STRING; 4932.YAL025C; -.
DR iPTMnet; P10962; -.
DR MaxQB; P10962; -.
DR PaxDb; P10962; -.
DR PRIDE; P10962; -.
DR EnsemblFungi; YAL025C_mRNA; YAL025C; YAL025C.
DR GeneID; 851208; -.
DR KEGG; sce:YAL025C; -.
DR SGD; S000000023; MAK16.
DR VEuPathDB; FungiDB:YAL025C; -.
DR eggNOG; KOG3064; Eukaryota.
DR GeneTree; ENSGT00390000012859; -.
DR HOGENOM; CLU_050888_0_1_1; -.
DR InParanoid; P10962; -.
DR OMA; GTYRDIY; -.
DR BioCyc; YEAST:G3O-28836-MON; -.
DR PRO; PR:P10962; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; P10962; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IBA:GO_Central.
DR GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR InterPro; IPR029004; L28e/Mak16.
DR InterPro; IPR006958; Mak16.
DR Pfam; PF04874; Mak16; 1.
DR Pfam; PF01778; Ribosomal_L28e; 1.
DR PIRSF; PIRSF003352; MAK16; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..306
FT /note="Protein MAK16"
FT /id="PRO_0000203793"
FT REPEAT 213..220
FT /note="1"
FT REPEAT 222..229
FT /note="2"
FT REGION 194..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..229
FT /note="2 X 8 AA repeats"
FT REGION 251..264
FT /note="7 X 2 AA repeats of S-[DE]"
FT MOTIF 139..144
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 282..287
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 197..230
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..250
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 250
FT /note="E -> Q (in Ref. 1; AAA34752 and 2; AAC05007)"
FT /evidence="ECO:0000305"
FT HELIX 4..11
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 18..20
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 26..28
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 48..58
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 67..70
FT /evidence="ECO:0007829|PDB:6EM3"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 86..96
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 97..99
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 102..116
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 117..120
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 121..126
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 141..144
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 145..148
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 149..156
FT /evidence="ECO:0007829|PDB:6EM3"
FT HELIX 158..170
FT /evidence="ECO:0007829|PDB:6EM3"
FT TURN 171..173
FT /evidence="ECO:0007829|PDB:6EM3"
SQ SEQUENCE 306 AA; 35696 MW; 309ED6897EA24F73 CRC64;
MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL
YLYMKTPERA HTPAKLWERI KLSKNYTKAL QQIDEHLLHW SKFFRHKCKQ RFTKLTQVMI
TERRLALREE ERHYVGVAPK VKRREQNRER KALVAAKIEK AIEKELMDRL KSGAYGDKPL
NVDEKVWKKI MGQMEEENSQ DEEEDWDEEE ESDDGEVEYV ADDGEGEYVD VDDLEKWLAD
SDREASSASE SESDSESESD SDSDEENKNS AKRRKKGTSA KTKRPKVEIE YEEEHEVQNA
EQEVAQ
