ID   MAKR5_ARATH             Reviewed;         326 AA.
AC   Q9FLX4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Membrane-associated kinase regulator 5 {ECO:0000303|PubMed:21289069};
GN   Name=MAKR5 {ECO:0000303|PubMed:21289069};
GN   OrderedLocusNames=At5g52870 {ECO:0000312|Araport:AT5G52870};
GN   ORFNames=MXC20.9 {ECO:0000312|EMBL:BAB10435.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=21289069; DOI=10.1101/gad.2001911;
RA   Jaillais Y., Hothorn M., Belkhadir Y., Dabi T., Nimchuk Z.L.,
RA   Meyerowitz E.M., Chory J.;
RT   "Tyrosine phosphorylation controls brassinosteroid receptor activation by
RT   triggering membrane release of its kinase inhibitor.";
RL   Genes Dev. 25:232-237(2011).
RN   [5]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27354416; DOI=10.15252/embr.201642450;
RA   Kang Y.H., Hardtke C.S.;
RT   "Arabidopsis MAKR5 is a positive effector of BAM3-dependent CLE45
RT   signaling.";
RL   EMBO Rep. 17:1145-1154(2016).
CC   -!- FUNCTION: Positive effector of CLE45 peptide signaling
CC       (PubMed:27354416). Post-transcriptionally regulated amplifier of the
CC       CLE45 peptide signal that acts downstream of BAM3 in the regulation of
CC       the transition of root protophloem cells from proliferation to
CC       differentiation; thus preventing primary root elongation but
CC       stimulating lateral roots development (PubMed:27354416).
CC       {ECO:0000269|PubMed:27354416}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27354416}.
CC       Cytoplasm, cytosol {ECO:0000269|PubMed:27354416}. Note=Recruited to the
CC       plasma membrane upon CLE45 peptide application.
CC       {ECO:0000269|PubMed:27354416}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:27354416}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates throughout roots vascular cylinders
CC       (PubMed:27354416). First observed early in the meristem, and later
CC       detected in procambial cells and phloem poles (PubMed:27354416). Absent
CC       from the xylem axis (PubMed:27354416). {ECO:0000269|PubMed:27354416}.
CC   -!- INDUCTION: Strong accumulation in developing sieve elements upon CLE45
CC       peptide application in a BAM3-dependent manner.
CC       {ECO:0000269|PubMed:27354416}.
CC   -!- DISRUPTION PHENOTYPE: Partial suppression of phenotypes observed in brx
CC       mutants such as reduced root growth and smaller root meristem size
CC       (PubMed:27354416). Reduced sensitivity to externally applied CLE45
CC       peptides in term of root growth suppression (PubMed:27354416).
CC       {ECO:0000269|PubMed:27354416}.
CC   -!- MISCELLANEOUS: Less stable in procambial than in phloem pole cells.
CC       {ECO:0000269|PubMed:27354416}.
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DR   EMBL; AB009055; BAB10435.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96270.1; -; Genomic_DNA.
DR   EMBL; AY045905; AAK76579.1; -; mRNA.
DR   EMBL; AY091212; AAM14151.1; -; mRNA.
DR   RefSeq; NP_200099.1; NM_124665.5.
DR   AlphaFoldDB; Q9FLX4; -.
DR   SMR; Q9FLX4; -.
DR   STRING; 3702.AT5G52870.1; -.
DR   iPTMnet; Q9FLX4; -.
DR   PaxDb; Q9FLX4; -.
DR   PRIDE; Q9FLX4; -.
DR   EnsemblPlants; AT5G52870.1; AT5G52870.1; AT5G52870.
DR   GeneID; 835364; -.
DR   Gramene; AT5G52870.1; AT5G52870.1; AT5G52870.
DR   KEGG; ath:AT5G52870; -.
DR   Araport; AT5G52870; -.
DR   TAIR; locus:2176867; AT5G52870.
DR   eggNOG; ENOG502RYGF; Eukaryota.
DR   HOGENOM; CLU_970943_0_0_1; -.
DR   InParanoid; Q9FLX4; -.
DR   OrthoDB; 1089112at2759; -.
DR   PhylomeDB; Q9FLX4; -.
DR   PRO; PR:Q9FLX4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FLX4; baseline and differential.
DR   Genevisible; Q9FLX4; AT.
DR   GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0045595; P:regulation of cell differentiation; IMP:UniProtKB.
DR   GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR   InterPro; IPR039619; MAKR2/5.
DR   PANTHER; PTHR33929; PTHR33929; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Membrane; Reference proteome.
FT   CHAIN           1..326
FT                   /note="Membrane-associated kinase regulator 5"
FT                   /id="PRO_0000410480"
FT   REGION          188..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          267..326
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        188..226
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..313
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   326 AA;  36486 MW;  576BB54AD6F82A18 CRC64;
     MEALTFMKFW LTNNTTIKPR REIRISESAV DSTTGSEDPD LDLCEGEDSF FELKISLSDF
     KTPKEKQRLE TTTTTTTYSV SNKSKVLPFV DISSKPQQSP TTLLKSGQKF RAFSFKKSEK
     STTTEKKKEE NNRTSLNVRF RVDDETTTTS FRKTASIARS QQTDDTMFDD SVSKRFFSLI
     KPLYTKSTKK QSSSTITSPT SSPATREKQR SNIPSGMRSV RRQLGKSRSA SAAIGGMSPA
     NRIDESLQVQ QDGIQSAILH CKKSFHGSRE SSLLSRSTSE SSSQEKLSTS SSEDSYLFSR
     ISSDSISEKS MDSLTSIKEQ REKISD