MAL1_DROME
ID MAL1_DROME Reviewed; 577 AA.
AC P07190; Q9V4T6;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 02-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Maltase A1;
DE EC=3.2.1.20;
DE AltName: Full=Larval visceral protein H;
DE Flags: Precursor;
GN Name=Mal-A1; Synonyms=LvpH; ORFNames=CG8696;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=6854639; DOI=10.1016/s0022-2836(83)80001-1;
RA Snyder M., Davidson N.;
RT "Two gene families clustered in a small region of the Drosophila genome.";
RL J. Mol. Biol. 166:101-118(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP SEQUENCE REVISION.
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-
CC glucose residues with release of alpha-D-glucose.; EC=3.2.1.20;
CC -!- DEVELOPMENTAL STAGE: One of the proteins expressed by the 44D cuticle
CC gene cluster. Expressed in first, second and early 3rd instar larvae
CC and in adults, but not in embryos or pupae.
CC {ECO:0000269|PubMed:6854639}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48097.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; V00204; CAA23491.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE013599; AAF59089.3; -; Genomic_DNA.
DR EMBL; AY070626; AAL48097.2; ALT_INIT; mRNA.
DR PIR; S07253; S07253.
DR RefSeq; NP_476627.3; NM_057279.5.
DR AlphaFoldDB; P07190; -.
DR SMR; P07190; -.
DR BioGRID; 61676; 5.
DR IntAct; P07190; 1.
DR STRING; 7227.FBpp0087838; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GlyGen; P07190; 5 sites.
DR PaxDb; P07190; -.
DR PRIDE; P07190; -.
DR DNASU; 35824; -.
DR EnsemblMetazoa; FBtr0088759; FBpp0087838; FBgn0002570.
DR GeneID; 35824; -.
DR KEGG; dme:Dmel_CG8696; -.
DR CTD; 35824; -.
DR FlyBase; FBgn0002570; Mal-A1.
DR VEuPathDB; VectorBase:FBgn0002570; -.
DR eggNOG; KOG0471; Eukaryota.
DR HOGENOM; CLU_006462_8_3_1; -.
DR InParanoid; P07190; -.
DR OMA; FYQIHPE; -.
DR OrthoDB; 1384693at2759; -.
DR PhylomeDB; P07190; -.
DR Reactome; R-DME-352230; Amino acid transport across the plasma membrane.
DR SignaLink; P07190; -.
DR BioGRID-ORCS; 35824; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35824; -.
DR PRO; PR:P07190; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0002570; Expressed in adult midgut (Drosophila) and 15 other tissues.
DR Genevisible; P07190; DM.
DR GO; GO:0032450; F:maltose alpha-glucosidase activity; ISS:FlyBase.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 3.90.400.10; -; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..577
FT /note="Maltase A1"
FT /id="PRO_0000001447"
FT ACT_SITE 221
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 297
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT SITE 363
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 244
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 315
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 331
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 577 AA; 66386 MW; E005AE05F98ABE0F CRC64;
MRPQSAACLL LAIVGFVGAT EWWESGNYYQ IYPRSFRDSD GDGIGDLNGV TEKLQYLKDI
GFTGTWLSPI FKSPMVDFGY DISDFYQIHP EYGTMEDFER MIAKAKEVGI KIILDFVPNH
SSTENEWFTK SVDSDPVYKD FYIWHDGKIN NETGEREPPS NWNSEFRYSA WEWNEVRQQY
YLHQFAIQQA DLNYRNPAVV NEMKNVIRFW LGKGVSGFRI DAVPYLFEVD LDRYNQYPDE
PLTNDSVNCP DPDDHCYTQH IYTQDMPETI DMVYQWRELV DEFHVENGGD KRLLMTEAYT
SFENIMTYYG NGVRNGSHIP FNFDFLTSIN NASKAGEYVE HIKKWMDAMP EGVYANWVLG
NHDNKRVASR FGVQRTDLIN ILLQTLPGHA VTYNGEELGM TDVWISWEDT VDPNACNSDP
DNYYARSRDP ARSPYQWDAS SKAGFTSADH TWLPVADDYK TNNALQQLRA PRSHLQIFKK
LVRVRKEPSF RQGELNIQAI DDDVIIYSRQ KTGSDLYVIV LNLGSTSKTL DLTKYYELGT
QAEVITTSLS SQYIDGDVIK STEFVANPYV GTVLVAV
