MAL2_DROME
ID MAL2_DROME Reviewed; 567 AA.
AC P07191; Q9V4T7;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Maltase A2;
DE EC=3.2.1.20;
DE AltName: Full=Larval visceral protein D;
DE Flags: Precursor;
GN Name=Mal-A2; Synonyms=LvpD; ORFNames=CG8694;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=6854639; DOI=10.1016/s0022-2836(83)80001-1;
RA Snyder M., Davidson N.;
RT "Two gene families clustered in a small region of the Drosophila genome.";
RL J. Mol. Biol. 166:101-118(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-
CC glucose residues with release of alpha-D-glucose.; EC=3.2.1.20;
CC -!- DEVELOPMENTAL STAGE: One of the proteins expressed by the 44D cuticle
CC gene cluster. Expressed in first, second and early 3rd instar larvae
CC and in adults, but not in embryos or pupae.
CC {ECO:0000269|PubMed:6854639}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA23492.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; V00204; CAA23492.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE013599; AAF59088.1; -; Genomic_DNA.
DR EMBL; AY071566; AAL49188.1; -; mRNA.
DR PIR; S08597; S08597.
DR RefSeq; NP_476625.2; NM_057277.3.
DR AlphaFoldDB; P07191; -.
DR SMR; P07191; -.
DR BioGRID; 61677; 5.
DR IntAct; P07191; 3.
DR STRING; 7227.FBpp0087826; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GlyGen; P07191; 4 sites.
DR PaxDb; P07191; -.
DR PRIDE; P07191; -.
DR DNASU; 35825; -.
DR EnsemblMetazoa; FBtr0088747; FBpp0087826; FBgn0002569.
DR GeneID; 35825; -.
DR KEGG; dme:Dmel_CG8694; -.
DR CTD; 35825; -.
DR FlyBase; FBgn0002569; Mal-A2.
DR VEuPathDB; VectorBase:FBgn0002569; -.
DR eggNOG; KOG0471; Eukaryota.
DR GeneTree; ENSGT00940000166852; -.
DR HOGENOM; CLU_006462_8_3_1; -.
DR InParanoid; P07191; -.
DR OMA; YSTARDV; -.
DR OrthoDB; 1384693at2759; -.
DR PhylomeDB; P07191; -.
DR Reactome; R-DME-352230; Amino acid transport across the plasma membrane.
DR SignaLink; P07191; -.
DR BioGRID-ORCS; 35825; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35825; -.
DR PRO; PR:P07191; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0002569; Expressed in adult midgut (Drosophila) and 12 other tissues.
DR Genevisible; P07191; DM.
DR GO; GO:0032450; F:maltose alpha-glucosidase activity; ISS:FlyBase.
DR Gene3D; 3.90.400.10; -; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..567
FT /note="Maltase A2"
FT /id="PRO_0000001446"
FT ACT_SITE 226
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 298
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT SITE 364
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 198
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 312
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 527
FT /note="P -> T (in Ref. 1; CAA23492)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 567 AA; 64542 MW; 51242BA9F185213D CRC64;
MPKWAHLGLA ALLLISTTQE GTADIDWWEN ASLYQIYPRS FQDSDGDGIG DLKGITSRLG
YLKEIGITAT WLSPIFTSPM SDFGYDISNF YDIDPIFGTL EDFDDLIVEA KSLGVKIILD
FVPNHSSDEN VWFEKSVNRE DGYDDFYVWD DGKLNEETGA RDPPSNWVSV FSGPMWTWNE
KRQQYFLHQF QVKQPDLNFT NPMVREHMLD VLKFWLDRGV DGFRIDAVPH IYEHRNADGS
YPDEPVSGWG SDPNAYDYHD HIYTKDQPAT VDLMYEWREF LDNYRAQNGG DSRVLLAEAY
SSVETLSAYF GNSTHQGTQL PMNFQLMYLS GYSTAKDVVG SIDYWMNTMW KEHQTANWVV
GNHDTNRVAD RMGAHKVDLL NVIVNALPGA SVTYYGEEIG MSNVDVECTG DSCEDRDGER
TPMQWTAGKN ADFSDGESTW LPLSPEYQRY NVQTERGVSR SSLNIFKGLQ ELKSSSAFLA
FKEDGGFSYE AVTEQVLQII RTNKISEEYR ILVNMGNGME ILDGLAPKTY EYVLATAYST
HYSGQKADLS QRIILMPYEA VVLRWLA
