MAL3_DROME
ID MAL3_DROME Reviewed; 574 AA.
AC P07192; Q9V4T8;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Maltase A3;
DE EC=3.2.1.20;
DE AltName: Full=Larval visceral protein L;
DE Flags: Precursor;
GN Name=Mal-A3; Synonyms=LvpL; ORFNames=CG8695;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=6854639; DOI=10.1016/s0022-2836(83)80001-1;
RA Snyder M., Davidson N.;
RT "Two gene families clustered in a small region of the Drosophila genome.";
RL J. Mol. Biol. 166:101-118(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP IDENTIFICATION.
RX PubMed=21082169; DOI=10.1007/s00239-010-9406-3;
RA Gabrisko M., Janecek S.;
RT "Characterization of maltase clusters in the genus Drosophila.";
RL J. Mol. Evol. 72:104-118(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-
CC glucose residues with release of alpha-D-glucose.; EC=3.2.1.20;
CC -!- DEVELOPMENTAL STAGE: One of the proteins expressed by the 44D cuticle
CC gene cluster. Expressed in first, second and early 3rd instar larvae
CC and in adults, but not in embryos or pupae.
CC {ECO:0000269|PubMed:6854639}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA23493.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; V00204; CAA23493.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE013599; AAF59087.2; -; Genomic_DNA.
DR EMBL; AY119654; AAM50308.1; -; mRNA.
DR PIR; S08598; S08598.
DR RefSeq; NP_476628.2; NM_057280.4.
DR AlphaFoldDB; P07192; -.
DR SMR; P07192; -.
DR IntAct; P07192; 1.
DR STRING; 7227.FBpp0087837; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GlyGen; P07192; 3 sites.
DR PaxDb; P07192; -.
DR PRIDE; P07192; -.
DR DNASU; 35826; -.
DR EnsemblMetazoa; FBtr0088758; FBpp0087837; FBgn0002571.
DR GeneID; 35826; -.
DR KEGG; dme:Dmel_CG8695; -.
DR CTD; 35826; -.
DR FlyBase; FBgn0002571; Mal-A3.
DR VEuPathDB; VectorBase:FBgn0002571; -.
DR eggNOG; KOG0471; Eukaryota.
DR GeneTree; ENSGT00940000166852; -.
DR HOGENOM; CLU_006462_8_3_1; -.
DR InParanoid; P07192; -.
DR OMA; MNNHDVP; -.
DR OrthoDB; 1384693at2759; -.
DR PhylomeDB; P07192; -.
DR Reactome; R-DME-352230; Amino acid transport across the plasma membrane.
DR BioGRID-ORCS; 35826; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35826; -.
DR PRO; PR:P07192; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0002571; Expressed in adult midgut (Drosophila) and 17 other tissues.
DR Genevisible; P07192; DM.
DR GO; GO:0032450; F:maltose alpha-glucosidase activity; ISS:FlyBase.
DR Gene3D; 3.90.400.10; -; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..574
FT /note="Maltase A3"
FT /id="PRO_0000001448"
FT ACT_SITE 220
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 293
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT SITE 359
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 307
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 443
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 146
FT /note="H -> P (in Ref. 1; CAA23493)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="Q -> E (in Ref. 1; CAA23493)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="K -> Q (in Ref. 1; CAA23493)"
FT /evidence="ECO:0000305"
FT CONFLICT 553
FT /note="N -> S (in Ref. 1; CAA23493)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 574 AA; 65235 MW; 6CDD5C92868DE098 CRC64;
MFKLLVLSCL LALALPSLAE VGWWKTGQFY QIYPRSFKDS DGDGVGDLIG ITQQLPYLKE
IGITATWLSP IFTSPMADFG YDVADLKGID PIFGTMEDFE ALLARAKELD IKIILDFVPN
HTSDECDWFI RSAAGEEEYK DFYVWHTGKV VNGIRQPPTN WVSVFRGSMW TWNEQRQAYY
LHQFHAKQPD LNYRNPKVVE AMKDVLRFWL RKGAYGFRID AVPHVYEIPA DADGNWPDEP
RNEAVSDPED YTYLQHIYTT DQPETLELVY AFRDVIEEID AELGGDDRVL LTEAYSPLEV
LMQYYGNGTH LGSQIPFNFE LLAKISYSSD AYHYSELIHN WLDNMPEGQV ANWVFGNHDQ
SRIGSRLGAD RIDACNMIIL GLPGVSVTYQ GEEMGMTDVW ISWEDTVDPQ ACQSNEQEFE
RLTRDPVRTP FQWSDEVNAG FSNASVTWLP VASNYKLVNV KKERGIALSH LNVYKQLRAL
RDEPTLKQGD VSVTAIGPNV LAFKRSLAGY KSYITLININ DDVESINLDS VFTSITTQLQ
YVVVNDKSVR RKNDLTFANS VLLLPKEAVV LSTV
