MALD3_MOUSE
ID MALD3_MOUSE Reviewed; 376 AA.
AC Q9D956; Q8R109;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=MARVEL domain-containing protein 3;
GN Name=Marveld3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=20028514; DOI=10.1186/1471-2121-10-95;
RA Steed E., Rodrigues N.T., Balda M.S., Matter K.;
RT "Identification of MarvelD3 as a tight junction-associated transmembrane
RT protein of the occludin family.";
RL BMC Cell Biol. 10:95-95(2009).
CC -!- FUNCTION: As a component of tight junctions, plays a role in
CC paracellular ion conductivity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cell junction, tight junction {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D956-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D956-2; Sequence=VSP_022323;
CC -!- TISSUE SPECIFICITY: Widely expressed with highest levels in small
CC intestine, colon, stomach and lung. Liver expresses only isoform 2.
CC {ECO:0000269|PubMed:20028514}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK007346; BAB24975.1; -; mRNA.
DR EMBL; BC025851; AAH25851.1; -; mRNA.
DR CCDS; CCDS22656.1; -. [Q9D956-2]
DR CCDS; CCDS22657.1; -. [Q9D956-1]
DR RefSeq; NP_082860.2; NM_028584.3.
DR RefSeq; NP_997612.2; NM_212447.2.
DR AlphaFoldDB; Q9D956; -.
DR iPTMnet; Q9D956; -.
DR PhosphoSitePlus; Q9D956; -.
DR PRIDE; Q9D956; -.
DR ProteomicsDB; 287302; -. [Q9D956-1]
DR ProteomicsDB; 287303; -. [Q9D956-2]
DR DNASU; 73608; -.
DR GeneID; 73608; -.
DR KEGG; mmu:73608; -.
DR CTD; 91862; -.
DR MGI; MGI:1920858; Marveld3.
DR InParanoid; Q9D956; -.
DR OrthoDB; 1155000at2759; -.
DR PhylomeDB; Q9D956; -.
DR BioGRID-ORCS; 73608; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Marveld3; mouse.
DR PRO; PR:Q9D956; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9D956; protein.
DR GO; GO:0005923; C:bicellular tight junction; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031435; F:mitogen-activated protein kinase kinase kinase binding; ISO:MGI.
DR GO; GO:0070830; P:bicellular tight junction assembly; ISO:MGI.
DR GO; GO:0045216; P:cell-cell junction organization; IMP:MGI.
DR GO; GO:0010633; P:negative regulation of epithelial cell migration; IDA:MGI.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IDA:MGI.
DR GO; GO:0046329; P:negative regulation of JNK cascade; ISO:MGI.
DR GO; GO:1902414; P:protein localization to cell junction; ISO:MGI.
DR GO; GO:0006970; P:response to osmotic stress; ISO:MGI.
DR InterPro; IPR008253; Marvel.
DR Pfam; PF01284; MARVEL; 1.
DR PROSITE; PS51225; MARVEL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell junction; Membrane; Reference proteome;
KW Tight junction; Transmembrane; Transmembrane helix.
FT CHAIN 1..376
FT /note="MARVEL domain-containing protein 3"
FT /id="PRO_0000271530"
FT TOPO_DOM 1..173
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..247
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..335
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 357..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 168..361
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT REGION 1..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..134
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 174..376
FT /note="ACWQMLKALLNLLILACSSVSYNSTGGYTGITSLGGIYYYQYGGAYSGFDGA
FT DGERAQQLDVQFYQLKLPTVTAAMAYSGALMTFSCLTLLAGALRVPWHCPLWLVIEGLM
FT DALIAGAYVPGLYFFFQHLSAAYSSDVCKERETLYQSKGYSGFNCGVHGGDIGAGVFAA
FT MAIGVFAVGAVLAFRGYRKVKKLKEKPTEMLEF -> GVVQIMEVILNAMVLICIVASY
FT FVLAGFSASFASGSGFGNNYYSPFEGTELEQVRQLDQQYTILRSPLIYGGVAVSLGLGV
FT LTMGVLLQGAKSLRKLPRRWLLLEAAFSLLAAVGYCVAIGFYLYAALRINSTDTCKTRE
FT RVYARKGLTWMNCQLAGTDGAAATFACFLVILYAASVVMALRAYREQKHYKDSQEQHRN
FT YRDVPEYLWSGTL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022323"
FT CONFLICT 116
FT /note="R -> P (in Ref. 2; AAH25851)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 42146 MW; BA1FAC06C883C5CC CRC64;
MKNTSGHREP RTRPRERDPD RRPHPDRDHH VERSRDRGGD RHRERNGDVR GNGDRRAGRE
QRTDRDQRQD RHRDAGHRAS EQRALEKSRQ SRARPEPWGP SWDAAPTPGP APWGPRELSQ
KHGLGRRGLE SERASERYVP TYSVPALQEE EYYQSEAEGL LDCHKCRYLC TGRACWQMLK
ALLNLLILAC SSVSYNSTGG YTGITSLGGI YYYQYGGAYS GFDGADGERA QQLDVQFYQL
KLPTVTAAMA YSGALMTFSC LTLLAGALRV PWHCPLWLVI EGLMDALIAG AYVPGLYFFF
QHLSAAYSSD VCKERETLYQ SKGYSGFNCG VHGGDIGAGV FAAMAIGVFA VGAVLAFRGY
RKVKKLKEKP TEMLEF
