ID   MALNA_MALOL             Reviewed;          10 AA.
AC   P86600;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   22-APR-2020, entry version 20.
DE   RecName: Full=Malanin chain A;
DE   Flags: Fragment;
OS   Malania oleifera.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Ximeniaceae; Malania.
OX   NCBI_TaxID=397392;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, GLYCOSYLATION, MASS SPECTROMETRY, AND
RP   TOXIC DOSE.
RC   TISSUE=Seed {ECO:0000269|PubMed:19341757};
RX   PubMed=19341757; DOI=10.1016/j.toxicon.2009.03.024;
RA   Yuan Y., Dai X., Wang D., Zeng X.;
RT   "Purification, characterization and cytotoxicity of malanin, a novel plant
RT   toxin from the seeds of Malania oleifera.";
RL   Toxicon 54:121-127(2009).
CC   -!- FUNCTION: Significantly inhibits growth and induces an apoptotic
CC       response in HeLa cells through cell-cycle arrest at S-phase. Exhibits
CC       highly cytotoxic activities against cancer cell and non-cancer cell
CC       lines producing IC(50) values of 0.15 nM for human cervical carcinoma
CC       cells (HeLa), 7.71 nM for rat pheochromocytoma-12 cells (PC-12), 11.20
CC       nM for human breast cancer cells (MCF-7), 15.80 nM for human leukemia
CC       cells (K562), 2.79 nM for African green monkey kidney cells (Vero) and
CC       3.92 nM for normal Madin-Darby canine kidney cells (MDCK).
CC       {ECO:0000269|PubMed:19341757}.
CC   -!- SUBUNIT: Heterodimer of an A chain and a B chain; disulfide-linked.
CC       {ECO:0000269|PubMed:19341757}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:19341757}.
CC   -!- MASS SPECTROMETRY: Mass=61875; Method=MALDI; Note=The measured mass is
CC       that of the heterodimer.; Evidence={ECO:0000269|PubMed:19341757};
CC   -!- TOXIC DOSE: LD(50) is 26.22 ug/kg by intraperitoneal injection into IRC
CC       strain mice. {ECO:0000269|PubMed:19341757}.
CC   -!- TOXIC DOSE: LD(50) is 43.11 mg/kg by intragingival injection into IRC
CC       strain mice. {ECO:0000269|PubMed:19341757}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Apoptosis; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Plant defense; Toxin.
FT   CHAIN           1..>10
FT                   /note="Malanin chain A"
FT                   /id="PRO_0000395440"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|PubMed:19341757"
SQ   SEQUENCE   10 AA;  1172 MW;  3B88E5A9D1A72327 CRC64;
     DYPKLTFTTS