MALQ_ECOLI
ID MALQ_ECOLI Reviewed; 694 AA.
AC P15977; Q2M781;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=4-alpha-glucanotransferase;
DE EC=2.4.1.25;
DE AltName: Full=Amylomaltase;
DE AltName: Full=Disproportionating enzyme;
DE Short=D-enzyme;
GN Name=malQ; Synonyms=malA; OrderedLocusNames=b3416, JW3379;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2845225; DOI=10.1111/j.1365-2958.1988.tb00053.x;
RA Pugsley A.P., Dubreuil C.;
RT "Molecular characterization of malQ, the structural gene for the
RT Escherichia coli enzyme amylomaltase.";
RL Mol. Microbiol. 2:473-479(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the disproportionating enzyme family.
CC {ECO:0000305}.
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DR EMBL; M32793; AAA24106.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58214.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76441.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77875.1; -; Genomic_DNA.
DR PIR; C65137; C65137.
DR RefSeq; NP_417875.1; NC_000913.3.
DR RefSeq; WP_000444342.1; NZ_LN832404.1.
DR PDB; 4S3P; X-ray; 2.80 A; A/B=1-688.
DR PDB; 4S3Q; X-ray; 2.10 A; A/B/C=1-688.
DR PDB; 4S3R; X-ray; 2.10 A; A=1-688.
DR PDBsum; 4S3P; -.
DR PDBsum; 4S3Q; -.
DR PDBsum; 4S3R; -.
DR AlphaFoldDB; P15977; -.
DR SMR; P15977; -.
DR BioGRID; 4261216; 131.
DR DIP; DIP-10147N; -.
DR IntAct; P15977; 6.
DR STRING; 511145.b3416; -.
DR CAZy; GH77; Glycoside Hydrolase Family 77.
DR jPOST; P15977; -.
DR PaxDb; P15977; -.
DR PRIDE; P15977; -.
DR EnsemblBacteria; AAC76441; AAC76441; b3416.
DR EnsemblBacteria; BAE77875; BAE77875; BAE77875.
DR GeneID; 947923; -.
DR KEGG; ecj:JW3379; -.
DR KEGG; eco:b3416; -.
DR PATRIC; fig|1411691.4.peg.3312; -.
DR EchoBASE; EB0556; -.
DR eggNOG; COG1640; Bacteria.
DR HOGENOM; CLU_022072_1_1_6; -.
DR InParanoid; P15977; -.
DR OMA; QYPNWRL; -.
DR PhylomeDB; P15977; -.
DR BioCyc; EcoCyc:AMYLOMALT-MON; -.
DR BioCyc; MetaCyc:AMYLOMALT-MON; -.
DR BRENDA; 2.4.1.25; 2026.
DR PRO; PR:P15977; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IDA:EcoCyc.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005980; P:glycogen catabolic process; IMP:EcoCyc.
DR GO; GO:0000025; P:maltose catabolic process; IDA:EcoCyc.
DR InterPro; IPR003385; Glyco_hydro_77.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR32438; PTHR32438; 1.
DR Pfam; PF02446; Glyco_hydro_77; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR TIGRFAMs; TIGR00217; malQ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cytoplasm; Glycosyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..694
FT /note="4-alpha-glucanotransferase"
FT /id="PRO_0000170125"
FT CONFLICT 22
FT /note="A -> P (in Ref. 1; AAA24106)"
FT /evidence="ECO:0000305"
FT CONFLICT 199..200
FT /note="SP -> TA (in Ref. 1; AAA24106)"
FT /evidence="ECO:0000305"
FT CONFLICT 380..381
FT /note="AE -> GT (in Ref. 1; AAA24106)"
FT /evidence="ECO:0000305"
FT CONFLICT 462
FT /note="G -> R (in Ref. 1; AAA24106)"
FT /evidence="ECO:0000305"
FT HELIX 4..12
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 26..28
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 31..40
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 56..61
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 72..81
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:4S3P"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 106..117
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 119..127
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 135..138
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 143..148
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 150..152
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 156..161
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 164..176
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 181..185
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 204..209
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 211..213
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 216..218
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 220..224
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 226..232
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 235..246
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 247..249
FT /evidence="ECO:0007829|PDB:4S3P"
FT HELIX 252..270
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 276..308
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 315..317
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 320..322
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 328..336
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 338..364
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 368..375
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 384..388
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 390..392
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 397..401
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 405..407
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 411..415
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 420..425
FT /evidence="ECO:0007829|PDB:4S3Q"
FT TURN 426..428
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 429..438
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 443..447
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 450..453
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 455..460
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 465..467
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 469..472
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 475..489
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 492..495
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 503..511
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 515..519
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 520..522
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 533..535
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 538..544
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 552..556
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 559..566
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 573..596
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 602..604
FT /evidence="ECO:0007829|PDB:4S3P"
FT HELIX 608..610
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 615..626
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 631..635
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 637..640
FT /evidence="ECO:0007829|PDB:4S3Q"
FT TURN 654..659
FT /evidence="ECO:0007829|PDB:4S3Q"
FT STRAND 664..666
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 667..670
FT /evidence="ECO:0007829|PDB:4S3Q"
FT HELIX 674..688
FT /evidence="ECO:0007829|PDB:4S3Q"
SQ SEQUENCE 694 AA; 78503 MW; 73FFB3F8E5619311 CRC64;
MESKRLDNAA LAAGISPNYI NAHGKPQSIS AETKRRLLDA MHQRTATKVA VTPVPNVMVY
TSGKKMPMVV EGSGEYSWLL TTEEGTQYKG HVTGGKAFNL PTKLPEGYHT LTLTQDDQRA
HCRVIVAPKR CYEPQALLNK QKLWGACVQL YTLRSEKNWG IGDFGDLKAM LVDVAKRGGS
FIGLNPIHAL YPANPESASP YSPSSRRWLN VIYIDVNAVE DFHLSEEAQA WWQLPTTQQT
LQQARDADWV DYSTVTALKM TALRMAWKGF AQRDDEQMAA FRQFVAEQGD SLFWQAAFDA
LHAQQVKEDE MRWGWPAWPE MYQNVDSPEV RQFCEEHRDD VDFYLWLQWL AYSQFAACWE
ISQGYEMPIG LYRDLAVGVA EGGAETWCDR ELYCLKASVG APPDILGPLG QNWGLPPMDP
HIITARAYEP FIELLRANMQ NCGALRIDHV MSMLRLWWIP YGETADQGAY VHYPVDDLLS
ILALESKRHR CMVIGEDLGT VPVEIVGKLR SSGVYSYKVL YFENDHEKTF RAPKAYPEQS
MAVAATHDLP TLRGYWECGD LTLGKTLGLY PDEVVLRGLY QDRELAKQGL LDALHKYGCL
PKRAGHKASL MSMTPTLNRG LQRYIADSNS ALLGLQPEDW LDMAEPVNIP GTSYQYKNWR
RKLSATLESM FADDGVNKLL KDLDRRRRAA AKKK
