ID   MALR_BACSU              Reviewed;         235 AA.
AC   O05251; Q795M2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Transcriptional regulatory protein MalR;
DE   AltName: Full=Malate response regulator;
GN   Name=malR; Synonyms=yufM; OrderedLocusNames=BSU31530;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9274030; DOI=10.1099/00221287-143-8-2769;
RA   Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H.,
RA   Duesterhoeft A., Pohl T.M., Weitzenegger T.;
RT   "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence
RT   of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).";
RL   Microbiology 143:2769-2774(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA   Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA   Fujita Y.;
RT   "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT   regulatory systems.";
RL   J. Bacteriol. 183:7365-7370(2001).
RN   [4]
RP   FUNCTION.
RX   PubMed=12949159; DOI=10.1099/mic.0.26257-0;
RA   Tanaka K., Kobayashi K., Ogasawara N.;
RT   "The Bacillus subtilis YufLM two-component system regulates the expression
RT   of the malate transporters MaeN (YufR) and YflS, and is essential for
RT   utilization of malate in minimal medium.";
RL   Microbiology 149:2317-2329(2003).
RN   [5]
RP   REGULATION OF MAEA.
RX   PubMed=12949160; DOI=10.1099/mic.0.26256-0;
RA   Doan T., Servant P., Tojo S., Yamaguchi H., Lerondel G., Yoshida K.,
RA   Fujita Y., Aymerich S.;
RT   "The Bacillus subtilis ywkA gene encodes a malic enzyme and its
RT   transcription is activated by the YufL/YufM two-component system in
RT   response to malate.";
RL   Microbiology 149:2331-2343(2003).
CC   -!- FUNCTION: Member of a two-component regulatory system MalK/MalR.
CC       Activates transcription of maeA, maeN and yflS in presence of malate by
CC       binding to their promoter region. {ECO:0000269|PubMed:11717295,
CC       ECO:0000269|PubMed:12949159}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and activated by MalK. {ECO:0000305}.
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DR   EMBL; Z93937; CAB07935.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15142.1; -; Genomic_DNA.
DR   PIR; B70009; B70009.
DR   RefSeq; NP_391031.1; NC_000964.3.
DR   RefSeq; WP_009968074.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O05251; -.
DR   SMR; O05251; -.
DR   STRING; 224308.BSU31530; -.
DR   jPOST; O05251; -.
DR   PaxDb; O05251; -.
DR   PRIDE; O05251; -.
DR   EnsemblBacteria; CAB15142; CAB15142; BSU_31530.
DR   GeneID; 937174; -.
DR   KEGG; bsu:BSU31530; -.
DR   PATRIC; fig|224308.179.peg.3418; -.
DR   eggNOG; COG4565; Bacteria.
DR   InParanoid; O05251; -.
DR   OMA; DVHGLQL; -.
DR   PhylomeDB; O05251; -.
DR   BioCyc; BSUB:BSU31530-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR024187; Sig_transdc_resp-reg_cit/mal.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   PANTHER; PTHR45526; PTHR45526; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF006171; RR_citrat_malat; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..235
FT                   /note="Transcriptional regulatory protein MalR"
FT                   /id="PRO_0000081138"
FT   DOMAIN          3..119
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        178..197
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         54
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   235 AA;  26965 MW;  ECF254A3ACB9AE9A CRC64;
     MINVLIVEDD PMVGELNKRY LSQIDGFQLK GIASSFQSAL HILGEHHIDL ILLDIYMPGK
     NGLELLTELR AQNEAVDVIV ISAASELDVI KKTLRYGAVD YLIKPFEFER FQTALSDYRR
     KQKVYSTHRN MSQKELDAEL FQKKEATEKV QLPKGLTKST LKLIWSSIQS FENESFTTED
     LAKHTEISQV SIRKYLKFLE DIQVLNVEMA YGTIGRPVFQ YNVNNSNING IKQYL