MAM33_YEAST
ID MAM33_YEAST Reviewed; 266 AA.
AC P40513; D6VVL4; Q6Q5P4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Mitochondrial acidic protein MAM33;
DE Flags: Precursor;
GN Name=MAM33; OrderedLocusNames=YIL070C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND
RP CHARACTERIZATION.
RX PubMed=9559539;
RX DOI=10.1002/(sici)1097-0061(19980315)14:4<303::aid-yea217>3.0.co;2-n;
RA Seytter T., Lottspeich F., Neupert W., Schwarz E.;
RT "Mam33p, an oligomeric, acidic protein in the mitochondrial matrix of
RT Saccharomyces cerevisiae is related to the human complement receptor gC1q-
RT R.";
RL Yeast 14:303-310(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Not known. Binds to the sorting sequence of cytochrome b2.
CC -!- SUBUNIT: Homotrimer or homotetramer.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- MISCELLANEOUS: Present with 4910 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the MAM33 family. {ECO:0000305}.
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DR EMBL; Z38060; CAA86153.1; -; Genomic_DNA.
DR EMBL; AY557860; AAS56186.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08480.1; -; Genomic_DNA.
DR PIR; S48409; S48409.
DR RefSeq; NP_012194.1; NM_001179420.1.
DR PDB; 3QV0; X-ray; 2.10 A; A=48-266.
DR PDBsum; 3QV0; -.
DR AlphaFoldDB; P40513; -.
DR SMR; P40513; -.
DR BioGRID; 34922; 238.
DR DIP; DIP-4925N; -.
DR IntAct; P40513; 44.
DR MINT; P40513; -.
DR STRING; 4932.YIL070C; -.
DR iPTMnet; P40513; -.
DR MaxQB; P40513; -.
DR PaxDb; P40513; -.
DR PRIDE; P40513; -.
DR EnsemblFungi; YIL070C_mRNA; YIL070C; YIL070C.
DR GeneID; 854740; -.
DR KEGG; sce:YIL070C; -.
DR SGD; S000001332; MAM33.
DR VEuPathDB; FungiDB:YIL070C; -.
DR eggNOG; KOG2536; Eukaryota.
DR GeneTree; ENSGT00390000018406; -.
DR HOGENOM; CLU_072692_0_1_1; -.
DR InParanoid; P40513; -.
DR OMA; CEYMMSK; -.
DR BioCyc; YEAST:G3O-31337-MON; -.
DR Reactome; R-SCE-8980692; RHOA GTPase cycle.
DR Reactome; R-SCE-9013106; RHOC GTPase cycle.
DR EvolutionaryTrace; P40513; -.
DR PRO; PR:P40513; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40513; protein.
DR GO; GO:0005759; C:mitochondrial matrix; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR GO; GO:0042256; P:mature ribosome assembly; IBA:GO_Central.
DR Gene3D; 3.10.280.10; -; 1.
DR InterPro; IPR003428; MAM33.
DR InterPro; IPR036561; MAM33_sf.
DR PANTHER; PTHR10826; PTHR10826; 1.
DR Pfam; PF02330; MAM33; 1.
DR SUPFAM; SSF54529; SSF54529; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Mitochondrion; Phosphoprotein;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..47
FT /note="Mitochondrion"
FT CHAIN 48..266
FT /note="Mitochondrial acidic protein MAM33"
FT /id="PRO_0000018589"
FT MOD_RES 91
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 129
FT /note="E -> G (in Ref. 4; AAS56186)"
FT /evidence="ECO:0000305"
FT HELIX 50..65
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 74..83
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 115..118
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 166..174
FT /evidence="ECO:0007829|PDB:3QV0"
FT TURN 175..178
FT /evidence="ECO:0007829|PDB:3QV0"
FT STRAND 179..190
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 191..195
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 199..207
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 214..216
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 219..231
FT /evidence="ECO:0007829|PDB:3QV0"
FT HELIX 236..265
FT /evidence="ECO:0007829|PDB:3QV0"
SQ SEQUENCE 266 AA; 30132 MW; C4D5B0D761104366 CRC64;
MFLRSVNRAV TRSILTTPKP AVVKSSWRVF TVANSKRCFT PAAIMRNQET QRVGDILQSE
LKIEKETLPE STSLDSFNDF LNKYKFSLVE TPGKNEAEIV RRTESGETVH VFFDVAQIAN
LPYNNAMDEN TEQNEDGINE DDFDALSDNF ANVNVVISKE SASEPAVSFE LLMNLQEGSF
YVDSATPYPS VDAALNQSAE AEITRELVYH GPPFSNLDEE LQESLEAYLE SRGVNEELAS
FISAYSEFKE NNEYISWLEK MKKFFH
