ID   MAMF_MAGSA              Reviewed;         111 AA.
AC   Q2W8R8;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Magnetosome protein MamF {ECO:0000305};
GN   Name=mamF; OrderedLocusNames=amb0953;
OS   Magnetospirillum magneticum (strain AMB-1 / ATCC 700264).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Magnetospirillum.
OX   NCBI_TaxID=342108;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=16303747; DOI=10.1093/dnares/dsi002;
RA   Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.;
RT   "Complete genome sequence of the facultative anaerobic magnetotactic
RT   bacterium Magnetospirillum sp. strain AMB-1.";
RL   DNA Res. 12:157-166(2005).
RN   [2]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=21414040; DOI=10.1111/j.1365-2958.2011.07631.x;
RA   Quinlan A., Murat D., Vali H., Komeili A.;
RT   "The HtrA/DegP family protease MamE is a bifunctional protein with roles in
RT   magnetosome protein localization and magnetite biomineralization.";
RL   Mol. Microbiol. 80:1075-1087(2011).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=22716969; DOI=10.1111/j.1365-2958.2012.08132.x;
RA   Murat D., Falahati V., Bertinetti L., Csencsits R., Koernig A., Downing K.,
RA   Faivre D., Komeili A.;
RT   "The magnetosome membrane protein, MmsF, is a major regulator of magnetite
RT   biomineralization in Magnetospirillum magneticum AMB-1.";
RL   Mol. Microbiol. 85:684-699(2012).
CC   -!- FUNCTION: Plays a role in regulating magnetite crystal size; partially
CC       redundant function with MmsF. {ECO:0000250|UniProtKB:Q6NE74}.
CC   -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC       {ECO:0000269|PubMed:21414040}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Tagged protein forms straight lines extending along
CC       most of the cell associated with its inner curvature, in the correct
CC       position to be filaments that are seen associated with magnetosomes. In
CC       a mamE disruption MamF mislocalizes as a linear punctate pattern.
CC       {ECO:0000269|PubMed:21414040}.
CC   -!- PTM: Probably subject to cleavage or degradation.
CC       {ECO:0000250|UniProtKB:Q6NE74}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the probable mamGFDC operon leads to
CC       a slight reduction in magnetic response and slightly narrower magnetite
CC       crystals. {ECO:0000269|PubMed:22716969}.
CC   -!- MISCELLANEOUS: This bacteria makes up to 20 cubo-octahedral
CC       magnetosomes of about 45 nm in diameter which contain membrane-bound
CC       crystals of magnetite (Fe(3)O(4)). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the magnetosome MamF/MmsF protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP007255; BAE49757.1; -; Genomic_DNA.
DR   RefSeq; WP_008620747.1; NC_007626.1.
DR   AlphaFoldDB; Q2W8R8; -.
DR   EnsemblBacteria; BAE49757; BAE49757; amb0953.
DR   KEGG; mag:amb0953; -.
DR   HOGENOM; CLU_095018_0_1_5; -.
DR   OMA; IMGWIAS; -.
DR   OrthoDB; 1774376at2; -.
DR   Proteomes; UP000007058; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0110146; C:magnetosome membrane; IDA:UniProtKB.
PE   3: Inferred from homology;
KW   Biomineralization; Magnetosome; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..111
FT                   /note="Magnetosome protein MamF"
FT                   /id="PRO_0000447796"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        39..50
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..111
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   111 AA;  12405 MW;  80D05620DDEDB3AB CRC64;
     MAEAILLETE NTPCGCRSYL MAGLSYLGIL CFVPLLMSRD DEYVYFHAKQ GLVLWMWSVL
     AMFALHLPLI GKWLFGFSSM GVLVLSVAGL ASVALRRTWR LPLVGYFVAL I