MAMG_MAGGM
ID MAMG_MAGGM Reviewed; 84 AA.
AC Q6NE75;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Magnetosome protein MamG {ECO:0000305};
GN Name=mamG {ECO:0000303|PubMed:13129949};
GN OrderedLocusNames=MGMSRv2__2395.1 {ECO:0000305}; ORFNames=mgIa15, MGR_4075;
OS Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS / MSR-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=431944;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT island.";
RL J. Bacteriol. 185:5779-5790(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT gryphiswaldense comprises a magnetosome island which undergoes frequent
RT rearrangements during stationary growth.";
RL J. Bacteriol. 187:7176-7184(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=17449609; DOI=10.1128/jb.00119-07;
RA Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA Reinhardt R., Schueler D.;
RT "Comparative genome analysis of four magnetotactic bacteria reveals a
RT complex set of group-specific genes implicated in magnetosome
RT biomineralization and function.";
RL J. Bacteriol. 189:4899-4910(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA Wang L., Li J.;
RT "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL Genome Announc. 2:0-0(2014).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=18539817; DOI=10.1128/aem.00231-08;
RA Lang C., Schueler D.;
RT "Expression of green fluorescent protein fused to magnetosome proteins in
RT microaerophilic magnetotactic bacteria.";
RL Appl. Environ. Microbiol. 74:4944-4953(2008).
RN [6]
RP FUNCTION, PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=17965152; DOI=10.1128/jb.01371-07;
RA Scheffel A., Gaerdes A., Gruenberg K., Wanner G., Schueler D.;
RT "The major magnetosome proteins MamGFDC are not essential for magnetite
RT biomineralization in Magnetospirillum gryphiswaldense but regulate the size
RT of magnetosome crystals.";
RL J. Bacteriol. 190:377-386(2008).
CC -!- FUNCTION: Plays a role in regulating magnetite crystal size.
CC {ECO:0000269|PubMed:17965152}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000269|PubMed:18539817}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes as a filament-like structure in a
CC straight line running through the center of the cell, in a position
CC that corresponds to magnetosomes. {ECO:0000269|PubMed:18539817}.
CC -!- INDUCTION: Part of the probable 4 gene mamGFDC operon.
CC {ECO:0000305|PubMed:13129949, ECO:0000305|PubMed:17965152}.
CC -!- DISRUPTION PHENOTYPE: Deletion of any 2 genes encoded in this operon
CC (mamC, mamD, mamF and mamG) decreases crystal size regardless of the
CC protein combination. Deletion of the mamGFDC operon leads to smaller
CC magnetite crystals in irregularly spaced chains, but no other phenotype
CC (PubMed:17965152). Deletion of approximately 80 kb of DNA, including
CC this operon, leads to cells that are non-magnetic, lack internal
CC membrane systems, grow poorly, have reduced mobility and take-up and
CC accumulate iron poorly (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:17965152}.
CC -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC -!- SIMILARITY: Belongs to the magnetosome MamG (TC 9.B.95) protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=HG794546; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; BX571797; CAE12018.1; -; Genomic_DNA.
DR EMBL; AM085146; CAJ30098.1; -; Genomic_DNA.
DR EMBL; CU459003; CAM78007.1; -; Genomic_DNA.
DR EMBL; HG794546; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q6NE75; -.
DR SMR; Q6NE75; -.
DR TCDB; 9.B.95.1.1; the mamg (lg)4 repeat (mamg) family.
DR KEGG; mgry:MSR1_03190; -.
DR Proteomes; UP000018922; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; IDA:UniProtKB.
PE 3: Inferred from homology;
KW Biomineralization; Magnetosome; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..84
FT /note="Magnetosome protein MamG"
FT /id="PRO_0000447799"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..40
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 41..48
FT /note="LG repeat"
FT /evidence="ECO:0000305"
SQ SEQUENCE 84 AA; 7715 MW; A7C2DCCD5F077744 CRC64;
MIKGIAGVGG TALGVGGGVA APPVSAAAVG STLLAGKGVC LGLGLGLGAW GPVLLGVAGL
ACAASLCDYL KNRKAQAEAS AEPA
