MAMH_MAGGM
ID MAMH_MAGGM Reviewed; 428 AA.
AC Q6NE63; V6F5J8;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Magnetosome protein MamH {ECO:0000305};
DE AltName: Full=Probable magnetosome permease MamH {ECO:0000305};
GN Name=mamH {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2381;
OS Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS / MSR-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=431944;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT island.";
RL J. Bacteriol. 185:5779-5790(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA Wang L., Li J.;
RT "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL Genome Announc. 2:0-0(2014).
RN [3]
RP MINIMAL MAGNETOSOME ISLAND.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA Voigt B., Schweder T., Schueler D.;
RT "Functional analysis of the magnetosome island in Magnetospirillum
RT gryphiswaldense: the mamAB operon is sufficient for magnetite
RT biomineralization.";
RL PLoS ONE 6:E25561-E25561(2011).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=23889511; DOI=10.1111/mmi.12317;
RA Raschdorf O., Mueller F.D., Posfai M., Plitzko J.M., Schueler D.;
RT "The magnetosome proteins MamX, MamZ and MamH are involved in redox control
RT of magnetite biomineralization in Magnetospirillum gryphiswaldense.";
RL Mol. Microbiol. 89:872-886(2013).
CC -!- FUNCTION: Required for correct biomineralization of the magnetosome;
CC probably transports some form of iron. Partially functionally redundant
CC with MamZ. {ECO:0000269|PubMed:23889511}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000250|UniProtKB:Q2W8R0}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: The first gene in the probable 17 gene mamAB operon.
CC {ECO:0000305|PubMed:13129949}.
CC -!- DISRUPTION PHENOTYPE: Slight decrease in magnetic response, has about
CC 30% fewer magnetosomes which are about 40% smaller than wild-type.
CC Double mamH-mamZ deletion cells have a poor magnetic response and very
CC few wild-type crystals; most cells have flake-like crystals
CC (PubMed:23889511). Deletion of approximately 80 kb of DNA, including
CC this operon, leads to cells that are non-magnetic, lack internal
CC membrane systems, grow poorly, have reduced mobility and take-up and
CC accumulate iron poorly (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:23889511}.
CC -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC form a minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22043287}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CDK99596.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BX571797; CAE12030.1; -; Genomic_DNA.
DR EMBL; HG794546; CDK99596.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q6NE63; -.
DR SMR; Q6NE63; -.
DR STRING; 1430440.MGMSRv2_2381; -.
DR TCDB; 2.A.1.43.1; the major facilitator superfamily (mfs).
DR EnsemblBacteria; CDK99596; CDK99596; MGMSRv2__2381.
DR KEGG; mgy:MGMSRv2__2381; -.
DR eggNOG; COG2211; Bacteria.
DR HOGENOM; CLU_642213_0_0_5; -.
DR Proteomes; UP000018922; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; ISS:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 2.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Biomineralization; Ion transport; Iron; Iron transport; Magnetosome;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..428
FT /note="Magnetosome protein MamH"
FT /id="PRO_0000447744"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 1
FT /note="M -> MTGM (in Ref. 2; CDK99596)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 428 AA; 45666 MW; 4D5B25C4741C5519 CRC64;
MEPGRSEVEG HQRNALYLLS ALCMVFMTLV VAIQPLFLRN VLNIPFETAG AVNANVQVVT
EVLDLFIFAY LGYLSDRIGR VRIIVAGFLV AAVGAVIAPL SPWVGGASIG ALVVYYVSRV
IMSAGSGAVW PQLSALAGDF SDDSNRARLL SNTAFMMAFG VTLVYAVLMQ IPGHAGITVT
MLLTAAISVA GAWLARKFLV DVAPRTVETS VPWRAVWDLV KAEPRLRLAF ASSLFARSDM
VFVGLFLMLW FIYFADLVKV GQAEAAARAG MLIGLMGAVV MLSIPVWRSF IERFGRIQAV
LLGMMLSALG FIMLGFVVNP FDGFIVFPIL LVSAGQAGCF VAPQILTVDH APKDLLGSVL
GAFNVIGCLG IIFFVQIGGF LFDYIGPPAP FIFTGVGNLI ISAYALRLVK SEARGVGGGN
TPGDDEVA
