MAMI_MAGGM
ID MAMI_MAGGM Reviewed; 69 AA.
AC Q6NE62; V6F240;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2019, sequence version 2.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Magnetosome protein MamI {ECO:0000305};
GN Name=mamI {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2380;
GN ORFNames=mgIa18, MGR_4090;
OS Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS / MSR-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=431944;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT island.";
RL J. Bacteriol. 185:5779-5790(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT gryphiswaldense comprises a magnetosome island which undergoes frequent
RT rearrangements during stationary growth.";
RL J. Bacteriol. 187:7176-7184(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=17449609; DOI=10.1128/jb.00119-07;
RA Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA Reinhardt R., Schueler D.;
RT "Comparative genome analysis of four magnetotactic bacteria reveals a
RT complex set of group-specific genes implicated in magnetosome
RT biomineralization and function.";
RL J. Bacteriol. 189:4899-4910(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA Wang L., Li J.;
RT "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL Genome Announc. 2:0-0(2014).
RN [5]
RP MINIMAL MAGNETOSOME ISLAND.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA Voigt B., Schweder T., Schueler D.;
RT "Functional analysis of the magnetosome island in Magnetospirillum
RT gryphiswaldense: the mamAB operon is sufficient for magnetite
RT biomineralization.";
RL PLoS ONE 6:E25561-E25561(2011).
RN [6]
RP SEQUENCE REVISION TO N-TERMINUS, AND DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24816605; DOI=10.1128/jb.01716-14;
RA Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA Faivre D., Baumgartner J., Schueler D.;
RT "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL J. Bacteriol. 196:2658-2669(2014).
RN [7]
RP FUNCTION, MINIMAL VESICLE FORMATION GENES, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=27286560; DOI=10.1371/journal.pgen.1006101;
RA Raschdorf O., Forstner Y., Kolinko I., Uebe R., Plitzko J.M., Schueler D.;
RT "Genetic and Ultrastructural Analysis Reveals the Key Players and Initial
RT Steps of Bacterial Magnetosome Membrane Biogenesis.";
RL PLoS Genet. 12:E1006101-E1006101(2016).
CC -!- FUNCTION: May be involved in an early stage of magnetosome nucleation
CC (Probable). Not essential for formation of magnetosome membrane
CC vesicles, it is probably functionally redundant with other proteins
CC (PubMed:27286560). May bind magnetite (By similarity). One of 7 genes
CC (mamLQBIEMO) able to induce magnetosome membrane biogenesis;
CC coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon
CC restores magnetosome vesicle formation but not magnetite biosynthesis
CC (PubMed:27286560). {ECO:0000250|UniProtKB:Q2W8Q9,
CC ECO:0000269|PubMed:27286560, ECO:0000305|PubMed:24816605}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000269|PubMed:27286560}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Tagged protein is found in punctate chains.
CC {ECO:0000269|PubMed:27286560}.
CC -!- INDUCTION: Part of the probable 17 gene mamAB operon.
CC {ECO:0000305|PubMed:13129949}.
CC -!- DISRUPTION PHENOTYPE: No magnetic response, fewer, smaller magnetosomes
CC which do not contain magnetite. MamC localizes at mid cell in a short
CC chain (PubMed:24816605). Magnetosome vesicles are approximately wild-
CC type in size, shape and chain-like alignment, but there are many fewer
CC vesicles, only a few have crystals (PubMed:27286560). Deletion of
CC approximately 80 kb of DNA, including this operon, leads to cells that
CC are non-magnetic, lack internal membrane systems, grow poorly, have
CC reduced mobility and take-up and accumulate iron poorly
CC (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:24816605, ECO:0000269|PubMed:27286560}.
CC -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC form a minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22043287}.
CC -!- SIMILARITY: Belongs to the magnetosome MamI protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE12031.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC Sequence=CAJ30115.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC Sequence=CAM78022.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
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DR EMBL; BX571797; CAE12031.1; ALT_INIT; Genomic_DNA.
DR EMBL; AM085146; CAJ30115.1; ALT_INIT; Genomic_DNA.
DR EMBL; CU459003; CAM78022.1; ALT_INIT; Genomic_DNA.
DR EMBL; HG794546; CDK99595.1; -; Genomic_DNA.
DR RefSeq; WP_024080591.1; NZ_CP027526.1.
DR AlphaFoldDB; Q6NE62; -.
DR SMR; Q6NE62; -.
DR STRING; 1430440.MGMSRv2_2380; -.
DR EnsemblBacteria; CDK99595; CDK99595; MGMSRv2__2380.
DR KEGG; mgry:MSR1_03350; -.
DR KEGG; mgy:MGMSRv2__2380; -.
DR eggNOG; ENOG502ZSFF; Bacteria.
DR HOGENOM; CLU_2788987_0_0_5; -.
DR Proteomes; UP000018922; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE 3: Inferred from homology;
KW Biomineralization; Iron; Magnetosome; Membrane; Metal-binding;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..69
FT /note="Magnetosome protein MamI"
FT /id="PRO_0000447801"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..31
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
SQ SEQUENCE 69 AA; 7156 MW; 406C37076D66A3DC CRC64;
MPSVIFGLLA LAIGLLGLTA WWWSVTEFLR GAVPVALIIF GLVALAAGVQ SVRVPPAGKR
ANSDPNIDG
