MAMJ_MAGSA
ID MAMJ_MAGSA Reviewed; 506 AA.
AC Q2W8Q7;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Magnetosome-associated protein MamJ {ECO:0000305};
GN Name=mamJ; OrderedLocusNames=amb0964;
OS Magnetospirillum magneticum (strain AMB-1 / ATCC 700264).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=342108;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=16303747; DOI=10.1093/dnares/dsi002;
RA Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.;
RT "Complete genome sequence of the facultative anaerobic magnetotactic
RT bacterium Magnetospirillum sp. strain AMB-1.";
RL DNA Res. 12:157-166(2005).
RN [2]
RP SUBCELLULAR LOCATION, PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=20212111; DOI=10.1073/pnas.0914439107;
RA Murat D., Quinlan A., Vali H., Komeili A.;
RT "Comprehensive genetic dissection of the magnetosome gene island reveals
RT the step-wise assembly of a prokaryotic organelle.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:5593-5598(2010).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=21883528; DOI=10.1111/j.1365-2958.2011.07815.x;
RA Draper O., Byrne M.E., Li Z., Keyhani S., Barrozo J.C., Jensen G.,
RA Komeili A.;
RT "MamK, a bacterial actin, forms dynamic filaments in vivo that are
RT regulated by the acidic proteins MamJ and LimJ.";
RL Mol. Microbiol. 82:342-354(2011).
RN [4]
RP MINIMAL MAGNETOSOME ISLAND.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=22716969; DOI=10.1111/j.1365-2958.2012.08132.x;
RA Murat D., Falahati V., Bertinetti L., Csencsits R., Koernig A., Downing K.,
RA Faivre D., Komeili A.;
RT "The magnetosome membrane protein, MmsF, is a major regulator of magnetite
RT biomineralization in Magnetospirillum magneticum AMB-1.";
RL Mol. Microbiol. 85:684-699(2012).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=27481925; DOI=10.1128/jb.00280-16;
RA Arakaki A., Kikuchi D., Tanaka M., Yamagishi A., Yoda T., Matsunaga T.;
RT "Comparative subcellular localization analysis of magnetosome proteins
RT reveals a unique localization behavior of Mms6 protein onto magnetite
RT crystals.";
RL J. Bacteriol. 198:2794-2802(2016).
CC -!- FUNCTION: Regulates the dynamic behavior of MamK filaments; paralog
CC LimJ also promotes MamK turnover. At least one other protein besides
CC MamJ and LimJ is required for MamK turnover (PubMed:21883528). May
CC connect magnetosomes to MamK filaments (Probable). Moves from the cell
CC poles towards midcell; movement does not depend on the treadmilling
CC ability of MamK, suggesting MamJ associates and disassociates
CC continuously from the MamK filament (By similarity).
CC {ECO:0000250|UniProtKB:V6F519, ECO:0000269|PubMed:21883528,
CC ECO:0000305|PubMed:21883528}.
CC -!- SUBUNIT: Forms homooligomers. Interacts with MamK.
CC {ECO:0000250|UniProtKB:V6F519}.
CC -!- SUBCELLULAR LOCATION: Magnetosome {ECO:0000269|PubMed:20212111,
CC ECO:0000269|PubMed:27481925}. Note=Tagged protein extends in a line
CC from one pole to the other, and is longer than the magnetosome chain.
CC {ECO:0000269|PubMed:20212111, ECO:0000269|PubMed:27481925}.
CC -!- INDUCTION: Part of the probable 18 gene mamAB operon.
CC {ECO:0000305|PubMed:20212111}.
CC -!- DOMAIN: The center of the protein encodes 8.6 repetitions of a Glu-rich
CC sequence. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Single gene deletion, no effect on magnetic
CC response or MamK filaments, gaps form in the magnetosome chain. A
CC double mamJ/limJ deletion has wild-type magnetic response, but forms
CC large gaps in the magnetosome chains reminiscent of the mamK deletion.
CC MamK forms bundles in these gaps, but the MamK filaments are no longer
CC dynamic (PubMed:21883528). Deletion of genes mamH to mamV (amb0961 to
CC amb0978) gives cells with no magnetosomes and no magnetic response
CC (PubMed:20212111). {ECO:0000269|PubMed:20212111,
CC ECO:0000269|PubMed:21883528}.
CC -!- MISCELLANEOUS: This bacteria makes up to 20 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (amb0961 to amb0978), including this gene, is sufficient to form a
CC minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22716969}.
CC -!- SIMILARITY: Belongs to the magnetosome MamJ protein family.
CC {ECO:0000305}.
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DR EMBL; AP007255; BAE49768.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2W8Q7; -.
DR STRING; 342108.amb0964; -.
DR EnsemblBacteria; BAE49768; BAE49768; amb0964.
DR KEGG; mag:amb0964; -.
DR HOGENOM; CLU_538390_0_0_5; -.
DR OMA; QGDIWAS; -.
DR Proteomes; UP000007058; Chromosome.
DR GO; GO:0110143; C:magnetosome; IDA:UniProtKB.
DR GO; GO:0110148; P:biomineralization; IMP:UniProtKB.
PE 3: Inferred from homology;
KW Biomineralization; Magnetosome; Reference proteome; Repeat.
FT CHAIN 1..506
FT /note="Magnetosome-associated protein MamJ"
FT /id="PRO_0000447804"
FT REPEAT 150..173
FT /note="Acidic repeat 1"
FT REPEAT 174..197
FT /note="Acidic repeat 2"
FT REPEAT 198..221
FT /note="Acidic repeat 3"
FT REPEAT 222..245
FT /note="Acidic repeat 4"
FT REPEAT 246..269
FT /note="Acidic repeat 5"
FT REPEAT 270..293
FT /note="Acidic repeat 6"
FT REPEAT 294..317
FT /note="Acidic repeat 7"
FT REPEAT 318..341
FT /note="Acidic repeat 8"
FT REPEAT 342..355
FT /note="Acidic repeat 8.5"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 63..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 405..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 506 AA; 52267 MW; D68A5E6FAD01B090 CRC64;
MANNRRDRDK GDGSQGEGLS AGGGMPSEPE IVSVTVHPTP TLAVSLKPAQ QGDIWASLLE
SSPWSANHGG RIEPVQPSPA PTRPTESPSV GDLVSRWSQP IWRTPSRVEE VETPESNFVI
ELEASQPITS DITVSVSDLV IEVQPIQTTE AEPEPELPVA EPEPGVVPVD VVAEAEPEPE
LPVAEPEPGV VPVDVVAEAE PEPELPVAEP EPEVVPVDVV AEAEPEPELP VAEPEPEVVP
VDVVAEAEPE PELPVAEPEP EVVPVDVVAE AEPEPELPVA APEPEVLPVE VVAEAEPEPE
LPVAAPEPEV LPVEVVAEAE PEPELPVAAP EPEVVPVDVV AEAEPEPELP VAEPELVHEA
AVSEPVAESI EDAVAVPAPA TNFGLAELMQ AAAAMAPVLA SPPSVAPAAK PVREPAKKRK
APPVRKKVAT VQDVPVEDLL GGIFGIAGSA VRSVLSLGAG VVGGVVKGGR VIGDNVVAGA
RRLTGSAEGS CGTCSTSQCD TVGKKK
