MAML_MAGGM
ID MAML_MAGGM Reviewed; 78 AA.
AC Q6NE58; V6F5J5;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2019, sequence version 2.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Magnetosome protein MamL {ECO:0000305};
DE Flags: Precursor;
GN Name=mamL {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2376;
GN ORFNames=mgIa19, MGR_4094;
OS Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS / MSR-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=431944;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT island.";
RL J. Bacteriol. 185:5779-5790(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT gryphiswaldense comprises a magnetosome island which undergoes frequent
RT rearrangements during stationary growth.";
RL J. Bacteriol. 187:7176-7184(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=17449609; DOI=10.1128/jb.00119-07;
RA Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA Reinhardt R., Schueler D.;
RT "Comparative genome analysis of four magnetotactic bacteria reveals a
RT complex set of group-specific genes implicated in magnetosome
RT biomineralization and function.";
RL J. Bacteriol. 189:4899-4910(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA Wang L., Li J.;
RT "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL Genome Announc. 2:0-0(2014).
RN [5]
RP MINIMAL MAGNETOSOME ISLAND, PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA Voigt B., Schweder T., Schueler D.;
RT "Functional analysis of the magnetosome island in Magnetospirillum
RT gryphiswaldense: the mamAB operon is sufficient for magnetite
RT biomineralization.";
RL PLoS ONE 6:E25561-E25561(2011).
RN [6]
RP SEQUENCE REVISION TO N-TERMINUS, AND DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24816605; DOI=10.1128/jb.01716-14;
RA Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA Faivre D., Baumgartner J., Schueler D.;
RT "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL J. Bacteriol. 196:2658-2669(2014).
RN [7]
RP FUNCTION, MINIMAL VESICLE FORMATION GENES, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF 63-LYS--ARG-78; 63-LYS--LYS-68;
RP 64-ARG-ARG-65; HIS-67; LYS-72 AND 77-LYS-ARG-78.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=27286560; DOI=10.1371/journal.pgen.1006101;
RA Raschdorf O., Forstner Y., Kolinko I., Uebe R., Plitzko J.M., Schueler D.;
RT "Genetic and Ultrastructural Analysis Reveals the Key Players and Initial
RT Steps of Bacterial Magnetosome Membrane Biogenesis.";
RL PLoS Genet. 12:E1006101-E1006101(2016).
CC -!- FUNCTION: Involved in magnetite crystal maturation, but not in
CC magnetosome vesicle tubulation or formation. One of 7 genes
CC (mamLQBIEMO) able to induce magnetosome membrane biogenesis;
CC coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon
CC restores magnetosome vesicle formation but not magnetite biosynthesis.
CC {ECO:0000269|PubMed:27286560}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000305|PubMed:27286560}; Single-pass membrane protein
CC {ECO:0000255}. Note=Tagged protein forms chains, aligned patches but
CC also unaligned patches in the cell; the chains and aligned patches are
CC in the correct position to be magnetosomes.
CC {ECO:0000269|PubMed:27286560}.
CC -!- INDUCTION: Part of the probable 17 gene mamAB operon.
CC {ECO:0000305|PubMed:13129949, ECO:0000305|PubMed:22043287}.
CC -!- DISRUPTION PHENOTYPE: No magnetic response, no magnetosomes, no iron
CC crystals of any sort. MamC is mislocalized in a few punctate spots
CC throughout the cell (PubMed:24816605). No magnetic response at 30
CC degrees Celsius, a weak response at lower temperature. At 30 degrees
CC Celsius magnetosome vesicles are smaller, sometimes align in a chain
CC with the filament, only a few have very small crystals. Other, possibly
CC precursor magnetosome vesicles are visible. MamC and MamI mislocalized
CC to 1 to a few patches in most cells (PubMed:27286560). Deletion of 3
CC consecutive genes (mamJ, mamK, mamL) leads to cells devoid of
CC magnetosomes or a magnetic response (PubMed:22043287). Deletion of
CC approximately 80 kb of DNA, including this operon, leads to cells that
CC are non-magnetic, lack internal membrane systems, grow poorly, have
CC reduced mobility and take-up and accumulate iron poorly
CC (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:22043287, ECO:0000269|PubMed:24816605,
CC ECO:0000269|PubMed:27286560}.
CC -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC form a minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22043287}.
CC -!- SIMILARITY: Belongs to the magnetosome MamL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE12035.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC Sequence=CAJ30119.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC Sequence=CAM78026.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
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DR EMBL; BX571797; CAE12035.1; ALT_INIT; Genomic_DNA.
DR EMBL; AM085146; CAJ30119.1; ALT_INIT; Genomic_DNA.
DR EMBL; CU459003; CAM78026.1; ALT_INIT; Genomic_DNA.
DR EMBL; HG794546; CDK99591.1; -; Genomic_DNA.
DR RefSeq; WP_024080587.1; NZ_CP027526.1.
DR AlphaFoldDB; Q6NE58; -.
DR SMR; Q6NE58; -.
DR STRING; 1430440.MGMSRv2_2376; -.
DR EnsemblBacteria; CDK99591; CDK99591; MGMSRv2__2376.
DR KEGG; mgry:MSR1_03390; -.
DR KEGG; mgy:MGMSRv2__2376; -.
DR HOGENOM; CLU_2617815_0_0_5; -.
DR OrthoDB; 1920249at2; -.
DR Proteomes; UP000018922; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Biomineralization; Magnetosome; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..78
FT /note="Magnetosome protein MamL"
FT /id="PRO_0000447806"
FT TOPO_DOM 23..38
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MUTAGEN 63..78
FT /note="KRRKHKLPGKNIVIKR->QQQQYQLPGQNIVIQQ: No magnetite
FT crystals, wild-type protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 63..68
FT /note="KRRKHK->QRRQHQ: Approximately wild-type magnetite
FT crystals, wild-type protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 64..65
FT /note="RR->QQ: Approximately wild-type magnetite crystals,
FT wild-type protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 67
FT /note="H->Y: Approximately wild-type magnetite crystals,
FT wild-type protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 72
FT /note="K->Q: Slightly smaller magnetite crystals, wild-type
FT protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 77..78
FT /note="KR->QQ: Fewer, slightly smaller magnetite crystals,
FT wild-type protein localization."
FT /evidence="ECO:0000269|PubMed:27286560"
SQ SEQUENCE 78 AA; 8586 MW; FF5D29BD51BA1996 CRC64;
MVRVIGSLVF GGLILLLASS NAHMVETRFG PLIMLAPHFV VLGITFFLGF AIGIVLVFAN
VMKRRKHKLP GKNIVIKR
