ID   MAML_MAGSA              Reviewed;          78 AA.
AC   Q2W8Q5;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Magnetosome protein MamL {ECO:0000305};
DE   Flags: Precursor;
GN   Name=mamL; OrderedLocusNames=amb0966;
OS   Magnetospirillum magneticum (strain AMB-1 / ATCC 700264).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Magnetospirillum.
OX   NCBI_TaxID=342108;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=16303747; DOI=10.1093/dnares/dsi002;
RA   Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.;
RT   "Complete genome sequence of the facultative anaerobic magnetotactic
RT   bacterium Magnetospirillum sp. strain AMB-1.";
RL   DNA Res. 12:157-166(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=20212111; DOI=10.1073/pnas.0914439107;
RA   Murat D., Quinlan A., Vali H., Komeili A.;
RT   "Comprehensive genetic dissection of the magnetosome gene island reveals
RT   the step-wise assembly of a prokaryotic organelle.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:5593-5598(2010).
RN   [3]
RP   MINIMAL MAGNETOSOME ISLAND.
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=22716969; DOI=10.1111/j.1365-2958.2012.08132.x;
RA   Murat D., Falahati V., Bertinetti L., Csencsits R., Koernig A., Downing K.,
RA   Faivre D., Komeili A.;
RT   "The magnetosome membrane protein, MmsF, is a major regulator of magnetite
RT   biomineralization in Magnetospirillum magneticum AMB-1.";
RL   Mol. Microbiol. 85:684-699(2012).
RN   [4]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=AMB-1 / ATCC 700264;
RX   PubMed=27481925; DOI=10.1128/jb.00280-16;
RA   Arakaki A., Kikuchi D., Tanaka M., Yamagishi A., Yoda T., Matsunaga T.;
RT   "Comparative subcellular localization analysis of magnetosome proteins
RT   reveals a unique localization behavior of Mms6 protein onto magnetite
RT   crystals.";
RL   J. Bacteriol. 198:2794-2802(2016).
CC   -!- FUNCTION: Essential for magnetosome formation.
CC       {ECO:0000269|PubMed:20212111}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:27481925,
CC       ECO:0000305|PubMed:20212111}; Single-pass membrane protein
CC       {ECO:0000255}. Magnetosome membrane {ECO:0000250|UniProtKB:Q6NE58};
CC       Single-pass membrane protein {ECO:0000255}. Note=May associate
CC       transiently with magnetosomes; in about 10% of cells, GFP tagged
CC       protein is aligned in dots or very short filaments similar to
CC       magnetosome distribution. {ECO:0000269|PubMed:20212111,
CC       ECO:0000269|PubMed:27481925}.
CC   -!- INDUCTION: Part of the probable 18 gene mamAB operon.
CC       {ECO:0000305|PubMed:20212111}.
CC   -!- DISRUPTION PHENOTYPE: Cells have no magnetic response and no
CC       magnetosome membranes (PubMed:20212111). Deletion of genes mamH to mamV
CC       (amb0961 to amb0978) gives cells with no magnetosomes and no magnetic
CC       response (PubMed:20212111). {ECO:0000269|PubMed:20212111}.
CC   -!- MISCELLANEOUS: This bacteria makes up to 20 cubo-octahedral
CC       magnetosomes of about 45 nm in diameter which contain membrane-bound
CC       crystals of magnetite (Fe(3)O(4)). {ECO:0000305}.
CC   -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC       (amb0961 to amb0978), including this gene, is sufficient to form a
CC       minimal magnetosome chain with small magnetite particles.
CC       {ECO:0000269|PubMed:22716969}.
CC   -!- SIMILARITY: Belongs to the magnetosome MamL family. {ECO:0000305}.
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DR   EMBL; AP007255; BAE49770.1; -; Genomic_DNA.
DR   RefSeq; WP_008620778.1; NC_007626.1.
DR   AlphaFoldDB; Q2W8Q5; -.
DR   SMR; Q2W8Q5; -.
DR   EnsemblBacteria; BAE49770; BAE49770; amb0966.
DR   KEGG; mag:amb0966; -.
DR   HOGENOM; CLU_2617815_0_0_5; -.
DR   OrthoDB; 1920249at2; -.
DR   Proteomes; UP000007058; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0110146; C:magnetosome membrane; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
PE   3: Inferred from homology;
KW   Biomineralization; Cell inner membrane; Cell membrane; Magnetosome;
KW   Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..78
FT                   /note="Magnetosome protein MamL"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000447807"
FT   TOPO_DOM        23..38
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        60..78
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   78 AA;  8360 MW;  4AF838D0DCE1BA22 CRC64;
     MVRLIGSLVF GGLIVLLASS NAHMVETRLG PLAMIAPHFV VLGITFFLGF AIGIVSVLAN
     VMTRRKQKSP GKSIVIKR