MAMN_MAGSA
ID MAMN_MAGSA Reviewed; 437 AA.
AC Q2W8Q3;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Magnetosome protein MamN {ECO:0000305};
DE AltName: Full=Putative ion transporter MamN {ECO:0000305};
GN Name=mamN; OrderedLocusNames=amb0968;
OS Magnetospirillum magneticum (strain AMB-1 / ATCC 700264).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=342108;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=16303747; DOI=10.1093/dnares/dsi002;
RA Matsunaga T., Okamura Y., Fukuda Y., Wahyudi A.T., Murase Y., Takeyama H.;
RT "Complete genome sequence of the facultative anaerobic magnetotactic
RT bacterium Magnetospirillum sp. strain AMB-1.";
RL DNA Res. 12:157-166(2005).
RN [2]
RP FUNCTION, PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=20212111; DOI=10.1073/pnas.0914439107;
RA Murat D., Quinlan A., Vali H., Komeili A.;
RT "Comprehensive genetic dissection of the magnetosome gene island reveals
RT the step-wise assembly of a prokaryotic organelle.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:5593-5598(2010).
RN [3]
RP MINIMAL MAGNETOSOME ISLAND.
RC STRAIN=AMB-1 / ATCC 700264;
RX PubMed=22716969; DOI=10.1111/j.1365-2958.2012.08132.x;
RA Murat D., Falahati V., Bertinetti L., Csencsits R., Koernig A., Downing K.,
RA Faivre D., Komeili A.;
RT "The magnetosome membrane protein, MmsF, is a major regulator of magnetite
RT biomineralization in Magnetospirillum magneticum AMB-1.";
RL Mol. Microbiol. 85:684-699(2012).
CC -!- FUNCTION: Plays a role in biomineralization; might regulate pH in the
CC magnetosome. {ECO:0000305|PubMed:20212111}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000250|UniProtKB:Q6NE56}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Part of the probable 18 gene mamAB operon.
CC {ECO:0000305|PubMed:20212111}.
CC -!- DISRUPTION PHENOTYPE: Cells have no magnetic response but still make
CC empty magnetosome membranes; magnetosome proteins MamA and MamE
CC localize normally (PubMed:20212111). Deletion of genes mamH to mamV
CC (amb0961 to amb0978) gives cells with no magnetosomes and no magnetic
CC response (PubMed:20212111). {ECO:0000269|PubMed:20212111}.
CC -!- MISCELLANEOUS: This bacteria makes up to 20 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (amb0961 to amb0978), including this gene, is sufficient to form a
CC minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22716969}.
CC -!- SIMILARITY: Belongs to the arsenite-antimonite (ArsB) efflux (TC
CC 2.A.45) family. {ECO:0000305}.
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DR EMBL; AP007255; BAE49772.1; -; Genomic_DNA.
DR RefSeq; WP_008620782.1; NC_007626.1.
DR AlphaFoldDB; Q2W8Q3; -.
DR SMR; Q2W8Q3; -.
DR STRING; 342108.amb0968; -.
DR EnsemblBacteria; BAE49772; BAE49772; amb0968.
DR KEGG; mag:amb0968; -.
DR HOGENOM; CLU_011920_4_0_5; -.
DR OMA; SKENTDY; -.
DR OrthoDB; 626718at2; -.
DR Proteomes; UP000007058; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; ISS:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR Pfam; PF03600; CitMHS; 1.
PE 3: Inferred from homology;
KW Biomineralization; Ion transport; Magnetosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..437
FT /note="Magnetosome protein MamN"
FT /id="PRO_0000447743"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 437 AA; 45764 MW; D48AF5DFE2F1E7A7 CRC64;
MIGLLTLAVF VATFAVIYRW AEGSHLAVLA GAAALVVIGT ISGSYTPVMA LRSVYFETLA
LIFGMAAISA LLARSGVYAY LAAGTAELSQ GQGRWILVMM ALVTYGISLA SNSLVTVAVV
VPVTLTVCFR TGIDPVPVII AEIIAANLGG ASTMIGDFPN MILASAGKLH FNDFIAGMMP
VCLILLAVML VFFERRLGDW KGSEIPVDPV WARGEALRHS AIDRRLLSYG LIIFGVTVAG
LILAGPLKVR PGWIAFVAGV TALGLGRFKD DEFFSACGGT DILFYGGLFV MVGALTSVGI
LDWAVNWLEG ITAAHDRVRA ILLMWMAAAV TIFVGGGTSA AVFAPVAATL RLDGDGQAAW
WALALGIMAG SVAALPGATA GSLAMTQYSG FVKRHPELAS AAAAGLQFTH REYVRWGMPL
MGIFLVLGTV YIAVLVG
