MAMQ_MAGGM
ID MAMQ_MAGGM Reviewed; 272 AA.
AC Q93DY8; V6F230;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Magnetosome protein MamQ {ECO:0000305};
GN Name=mamQ {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2370;
GN ORFNames=mgI496, MGR_4100;
OS Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS / MSR-1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Magnetospirillum.
OX NCBI_TaxID=431944;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=11571158; DOI=10.1128/aem.67.10.4573-4582.2001;
RA Grunberg K., Wawer C., Tebo B.M., Schuler D.;
RT "A large gene cluster encoding several magnetosome proteins is conserved in
RT different species of magnetotactic bacteria.";
RL Appl. Environ. Microbiol. 67:4573-4582(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT island.";
RL J. Bacteriol. 185:5779-5790(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT gryphiswaldense comprises a magnetosome island which undergoes frequent
RT rearrangements during stationary growth.";
RL J. Bacteriol. 187:7176-7184(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=17449609; DOI=10.1128/jb.00119-07;
RA Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA Reinhardt R., Schueler D.;
RT "Comparative genome analysis of four magnetotactic bacteria reveals a
RT complex set of group-specific genes implicated in magnetosome
RT biomineralization and function.";
RL J. Bacteriol. 189:4899-4910(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA Wang L., Li J.;
RT "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL Genome Announc. 2:0-0(2014).
RN [6]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=14766587; DOI=10.1128/aem.70.2.1040-1050.2004;
RA Gruenberg K., Mueller E.C., Otto A., Reszka R., Linder D., Kube M.,
RA Reinhardt R., Schueler D.;
RT "Biochemical and proteomic analysis of the magnetosome membrane in
RT Magnetospirillum gryphiswaldense.";
RL Appl. Environ. Microbiol. 70:1040-1050(2004).
RN [7]
RP MINIMAL MAGNETOSOME ISLAND.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA Voigt B., Schweder T., Schueler D.;
RT "Functional analysis of the magnetosome island in Magnetospirillum
RT gryphiswaldense: the mamAB operon is sufficient for magnetite
RT biomineralization.";
RL PLoS ONE 6:E25561-E25561(2011).
RN [8]
RP DISRUPTION PHENOTYPE.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=24816605; DOI=10.1128/jb.01716-14;
RA Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA Faivre D., Baumgartner J., Schueler D.;
RT "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL J. Bacteriol. 196:2658-2669(2014).
RN [9]
RP FUNCTION, MINIMAL VESICLE FORMATION GENES, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, PUTATIVE TOPOLOGY, AND MUTAGENESIS OF GLU-111; GLU-179; TYR-181
RP AND 241-TYR-PHE-242.
RC STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX PubMed=27286560; DOI=10.1371/journal.pgen.1006101;
RA Raschdorf O., Forstner Y., Kolinko I., Uebe R., Plitzko J.M., Schueler D.;
RT "Genetic and Ultrastructural Analysis Reveals the Key Players and Initial
RT Steps of Bacterial Magnetosome Membrane Biogenesis.";
RL PLoS Genet. 12:E1006101-E1006101(2016).
CC -!- FUNCTION: Essential for magnetosome formation (PubMed:13129949). Not
CC essential for formation of magnetosome membrane vesicles. One of 7
CC genes (mamLQBIEMO) able to induce magnetosome membrane biogenesis;
CC coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon
CC restores magnetosome vesicle formation but not magnetite biosynthesis
CC (PubMed:27286560). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:27286560}.
CC -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC {ECO:0000269|PubMed:14766587, ECO:0000269|PubMed:27286560}; Single-pass
CC membrane protein {ECO:0000255}. Cell inner membrane
CC {ECO:0000269|PubMed:27286560}; Single-pass membrane protein
CC {ECO:0000255}. Note=Tagged protein is more abundant in cell inner
CC membrane, is visible as puncate spots all throughout inner and
CC magnetosome membranes. {ECO:0000269|PubMed:27286560}.
CC -!- INDUCTION: Part of the probable 17 gene mamAB operon.
CC {ECO:0000305|PubMed:13129949}.
CC -!- DISRUPTION PHENOTYPE: No magnetic response, no magnetosomes, no iron
CC crystals of any sort. MamC is mislocalized in punctate spots throughout
CC the cell (PubMed:24816605). Magnetosome vesicles are fewer and smaller,
CC sometimes align in a chain with the filament, only a few have very
CC small crystals. Many other, possibly precursor magnetosome vesicles,
CC are visible, some are attached to the cell inner membrane. MamI
CC mislocalized to 1 to a few patches in most cells (PubMed:27286560).
CC Deletion of approximately 80 kb of DNA, including this operon, leads to
CC cells that are non-magnetic, lack internal membrane systems, grow
CC poorly, have reduced mobility and take-up and accumulate iron poorly
CC (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC ECO:0000269|PubMed:24816605, ECO:0000269|PubMed:27286560}.
CC -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC magnetosomes of about 45 nm in diameter which contain membrane-bound
CC crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:11571158}.
CC -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC form a minimal magnetosome chain with small magnetite particles.
CC {ECO:0000269|PubMed:22043287}.
CC -!- SIMILARITY: Belongs to the LemA family. {ECO:0000305}.
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DR EMBL; AF374354; AAL09997.1; -; Genomic_DNA.
DR EMBL; BX571797; CAE12041.1; -; Genomic_DNA.
DR EMBL; AM085146; CAJ30125.1; -; Genomic_DNA.
DR EMBL; CU459003; CAM78032.1; -; Genomic_DNA.
DR EMBL; HG794546; CDK99585.1; -; Genomic_DNA.
DR RefSeq; WP_024080581.1; NZ_CP027526.1.
DR AlphaFoldDB; Q93DY8; -.
DR SMR; Q93DY8; -.
DR STRING; 1430440.MGMSRv2_2370; -.
DR EnsemblBacteria; CDK99585; CDK99585; MGMSRv2__2370.
DR KEGG; mgry:MSR1_03450; -.
DR KEGG; mgy:MGMSRv2__2370; -.
DR eggNOG; COG1704; Bacteria.
DR HOGENOM; CLU_056714_0_0_5; -.
DR OrthoDB; 1573349at2; -.
DR Proteomes; UP000018922; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110146; C:magnetosome membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 1.20.1440.20; -; 1.
DR InterPro; IPR023353; LemA-like_dom_sf.
DR InterPro; IPR007156; MamQ_LemA.
DR PANTHER; PTHR34478; PTHR34478; 1.
DR Pfam; PF04011; LemA; 1.
DR SUPFAM; SSF140478; SSF140478; 1.
PE 1: Evidence at protein level;
KW Biomineralization; Cell inner membrane; Cell membrane; Magnetosome;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..272
FT /note="Magnetosome protein MamQ"
FT /id="PRO_0000447750"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..272
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:27286560"
FT MUTAGEN 111
FT /note="E->A: Does not restore magnetic response."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 179
FT /note="E->A: Does not restore magnetic response."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 181
FT /note="Y->A: Does not restore magnetic response."
FT /evidence="ECO:0000269|PubMed:27286560"
FT MUTAGEN 241..242
FT /note="YF->AA: Does not restore magnetic response."
FT /evidence="ECO:0000269|PubMed:27286560"
SQ SEQUENCE 272 AA; 30003 MW; EAE5C5170768A6A6 CRC64;
MAVSDADASS VDKVESITLQ RVKQSEELLA QLYVVEESPR RMGRGPVQLM LAISVLSLVA
FITTLLMRYN AFVTMYEDAQ AKRSNFEVMI QRRDNLFGNL VKLTLNHAAL EHSIFSHTSD
KRKESVEAGK GGPIGSAIEQ LMKQGGIGKL LGDGGAGKAL LGADGGFGNA LGRLMAIVEQ
YPTVQSADTY KHMMTSLVDM EDRIASKREE FNASAATYNV AITKWPWDYL AMITGFKRVE
YFHEKPAGDT PIITPQIFQE LLPLTHSQES KN
