ID   MAMR_MAGGM              Reviewed;          84 AA.
AC   Q6NE51; V6F2A7;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 2.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Magnetosome protein MamR {ECO:0000305};
GN   Name=mamR {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2369;
GN   ORFNames=mgI497, MGR_4101;
OS   Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS   / MSR-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Magnetospirillum.
OX   NCBI_TaxID=431944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA   Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA   Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT   "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT   gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT   island.";
RL   J. Bacteriol. 185:5779-5790(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA   Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT   "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT   gryphiswaldense comprises a magnetosome island which undergoes frequent
RT   rearrangements during stationary growth.";
RL   J. Bacteriol. 187:7176-7184(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=17449609; DOI=10.1128/jb.00119-07;
RA   Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA   Reinhardt R., Schueler D.;
RT   "Comparative genome analysis of four magnetotactic bacteria reveals a
RT   complex set of group-specific genes implicated in magnetosome
RT   biomineralization and function.";
RL   J. Bacteriol. 189:4899-4910(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA   Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA   Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA   Wang L., Li J.;
RT   "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL   Genome Announc. 2:0-0(2014).
RN   [5]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=14766587; DOI=10.1128/aem.70.2.1040-1050.2004;
RA   Gruenberg K., Mueller E.C., Otto A., Reszka R., Linder D., Kube M.,
RA   Reinhardt R., Schueler D.;
RT   "Biochemical and proteomic analysis of the magnetosome membrane in
RT   Magnetospirillum gryphiswaldense.";
RL   Appl. Environ. Microbiol. 70:1040-1050(2004).
RN   [6]
RP   MINIMAL MAGNETOSOME ISLAND.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA   Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA   Voigt B., Schweder T., Schueler D.;
RT   "Functional analysis of the magnetosome island in Magnetospirillum
RT   gryphiswaldense: the mamAB operon is sufficient for magnetite
RT   biomineralization.";
RL   PLoS ONE 6:E25561-E25561(2011).
RN   [7]
RP   SEQUENCE REVISION TO N-TERMINUS, FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24816605; DOI=10.1128/jb.01716-14;
RA   Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA   Faivre D., Baumgartner J., Schueler D.;
RT   "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT   essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL   J. Bacteriol. 196:2658-2669(2014).
RN   [8]
RP   FUNCTION, AND MINIMAL VESICLE FORMATION GENES.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=27286560; DOI=10.1371/journal.pgen.1006101;
RA   Raschdorf O., Forstner Y., Kolinko I., Uebe R., Plitzko J.M., Schueler D.;
RT   "Genetic and Ultrastructural Analysis Reveals the Key Players and Initial
RT   Steps of Bacterial Magnetosome Membrane Biogenesis.";
RL   PLoS Genet. 12:E1006101-E1006101(2016).
CC   -!- FUNCTION: May play a role in controlling magnetite number and size
CC       (Probable). Coexpression of mamLQRBIEMO in a deletion of the 17 gene
CC       mamAB operon restores magnetosome vesicle formation but not magnetite
CC       biosynthesis (PubMed:27286560). {ECO:0000269|PubMed:27286560,
CC       ECO:0000305|PubMed:24816605}.
CC   -!- SUBCELLULAR LOCATION: Magnetosome {ECO:0000269|PubMed:14766587}.
CC   -!- INDUCTION: Part of the probable 17 gene mamAB operon.
CC       {ECO:0000305|PubMed:13129949}.
CC   -!- DISRUPTION PHENOTYPE: Normal magnetic response, slightly smaller,
CC       sometime scattered magnetosomes (PubMed:24816605). Deletion of
CC       approximately 80 kb of DNA, including this operon, leads to cells that
CC       are non-magnetic, lack internal membrane systems, grow poorly, have
CC       reduced mobility and take-up and accumulate iron poorly
CC       (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC       ECO:0000269|PubMed:24816605}.
CC   -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC       magnetosomes of about 45 nm in diameter which contain membrane-bound
CC       crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC   -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC       (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC       form a minimal magnetosome chain with small magnetite particles.
CC       {ECO:0000269|PubMed:22043287}.
CC   -!- SIMILARITY: Belongs to the magnetosome MamR family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE12042.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC       Sequence=CAJ30126.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
CC       Sequence=CAM78033.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:24816605};
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DR   EMBL; BX571797; CAE12042.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AM085146; CAJ30126.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CU459003; CAM78033.1; ALT_INIT; Genomic_DNA.
DR   EMBL; HG794546; CDK99584.1; -; Genomic_DNA.
DR   RefSeq; WP_024080580.1; NZ_CP027526.1.
DR   AlphaFoldDB; Q6NE51; -.
DR   STRING; 1430440.MGMSRv2_2369; -.
DR   EnsemblBacteria; CDK99584; CDK99584; MGMSRv2__2369.
DR   KEGG; mgy:MGMSRv2__2369; -.
DR   HOGENOM; CLU_2523586_0_0_5; -.
DR   OrthoDB; 1858791at2; -.
DR   Proteomes; UP000018922; Chromosome.
DR   GO; GO:0110143; C:magnetosome; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   InterPro; IPR041657; HTH_17.
DR   InterPro; IPR010093; SinI_DNA-bd.
DR   Pfam; PF12728; HTH_17; 1.
DR   TIGRFAMs; TIGR01764; excise; 1.
PE   1: Evidence at protein level;
KW   Biomineralization; Magnetosome; Reference proteome.
FT   CHAIN           1..84
FT                   /note="Magnetosome protein MamR"
FT                   /id="PRO_0000447808"
SQ   SEQUENCE   84 AA;  9330 MW;  CE51D424A2060F73 CRC64;
     MIWTAVIKGS ALMTFVQGAM ALVDKVFGEE ILPHRIYSSG EAAQLLGMER LQVLEMVRAG
     TIKAKKVGDN YRILGSNLVE YMNR