ID   MAMS_MAGGM              Reviewed;         180 AA.
AC   Q6NE49; V6F2G8;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Magnetosome protein MamS {ECO:0000305};
GN   Name=mamS {ECO:0000303|PubMed:13129949}; OrderedLocusNames=MGMSRv2__2367;
GN   ORFNames=mgI500, MGR_4103;
OS   Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS   / MSR-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Magnetospirillum.
OX   NCBI_TaxID=431944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA   Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA   Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT   "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT   gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT   island.";
RL   J. Bacteriol. 185:5779-5790(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA   Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT   "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT   gryphiswaldense comprises a magnetosome island which undergoes frequent
RT   rearrangements during stationary growth.";
RL   J. Bacteriol. 187:7176-7184(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=17449609; DOI=10.1128/jb.00119-07;
RA   Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA   Reinhardt R., Schueler D.;
RT   "Comparative genome analysis of four magnetotactic bacteria reveals a
RT   complex set of group-specific genes implicated in magnetosome
RT   biomineralization and function.";
RL   J. Bacteriol. 189:4899-4910(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA   Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA   Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA   Wang L., Li J.;
RT   "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL   Genome Announc. 2:0-0(2014).
RN   [5]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=14766587; DOI=10.1128/aem.70.2.1040-1050.2004;
RA   Gruenberg K., Mueller E.C., Otto A., Reszka R., Linder D., Kube M.,
RA   Reinhardt R., Schueler D.;
RT   "Biochemical and proteomic analysis of the magnetosome membrane in
RT   Magnetospirillum gryphiswaldense.";
RL   Appl. Environ. Microbiol. 70:1040-1050(2004).
RN   [6]
RP   MINIMAL MAGNETOSOME ISLAND.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA   Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA   Voigt B., Schweder T., Schueler D.;
RT   "Functional analysis of the magnetosome island in Magnetospirillum
RT   gryphiswaldense: the mamAB operon is sufficient for magnetite
RT   biomineralization.";
RL   PLoS ONE 6:E25561-E25561(2011).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24816605; DOI=10.1128/jb.01716-14;
RA   Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA   Faivre D., Baumgartner J., Schueler D.;
RT   "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT   essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL   J. Bacteriol. 196:2658-2669(2014).
CC   -!- FUNCTION: May play a role in magnetite crystal growth and size.
CC       {ECO:0000305|PubMed:24816605}.
CC   -!- SUBCELLULAR LOCATION: Magnetosome membrane
CC       {ECO:0000305|PubMed:14766587}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Part of the probable 17 gene mamAB operon.
CC       {ECO:0000305|PubMed:13129949}.
CC   -!- DISRUPTION PHENOTYPE: Normal magnetic response, smaller, widely spaced
CC       magnetosomes. Near wild-type localization of MamC (PubMed:24816605).
CC       Deletion of approximately 80 kb of DNA, including this operon, leads to
CC       cells that are non-magnetic, lack internal membrane systems, grow
CC       poorly, have reduced mobility and take-up and accumulate iron poorly
CC       (PubMed:13129949). {ECO:0000269|PubMed:13129949,
CC       ECO:0000269|PubMed:24816605}.
CC   -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC       magnetosomes of about 45 nm in diameter which contain membrane-bound
CC       crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
CC   -!- MISCELLANEOUS: Expression of just the minimal mamAB gene cluster
CC       (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to
CC       form a minimal magnetosome chain with small magnetite particles.
CC       {ECO:0000269|PubMed:22043287}.
CC   -!- SIMILARITY: Belongs to the magnetosome MamS family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX571797; CAE12044.1; -; Genomic_DNA.
DR   EMBL; AM085146; CAJ30128.1; -; Genomic_DNA.
DR   EMBL; CU459003; CAM78035.1; -; Genomic_DNA.
DR   EMBL; HG794546; CDK99582.1; -; Genomic_DNA.
DR   RefSeq; WP_024080578.1; NZ_CP027526.1.
DR   AlphaFoldDB; Q6NE49; -.
DR   SMR; Q6NE49; -.
DR   STRING; 1430440.MGMSRv2_2367; -.
DR   EnsemblBacteria; CDK99582; CDK99582; MGMSRv2__2367.
DR   KEGG; mgry:MSR1_03480; -.
DR   KEGG; mgy:MGMSRv2__2367; -.
DR   eggNOG; ENOG502ZUSW; Bacteria.
DR   HOGENOM; CLU_1494549_0_0_5; -.
DR   OrthoDB; 1521421at2; -.
DR   Proteomes; UP000018922; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0110146; C:magnetosome membrane; IMP:UniProtKB.
PE   3: Inferred from homology;
KW   Biomineralization; Magnetosome; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..180
FT                   /note="Magnetosome protein MamS"
FT                   /id="PRO_0000447810"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        43..180
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   180 AA;  18714 MW;  F2043212EBCFE1CC CRC64;
     MDFRPDQVVA RIRGAVEGAL TAQSVLGIGG ALVLILVVIA LLPDRFTRGE GKTATAVSSG
     AAQALPAALP GLSPFTPAKP LQFSGRVTQV ASIGNDVGWG QVHVWIDNGT GALQEISVAP
     QSYLNQIGCP SFDGARISGI GFLFDAGRPN AELYAKSVLV GGRTCKLRDD EGLALWMTVQ