MAN3_ARATH
ID MAN3_ARATH Reviewed; 414 AA.
AC Q9SG94;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Mannan endo-1,4-beta-mannosidase 3;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 3;
DE AltName: Full=Endo-beta-1,4-mannanase 3;
DE Short=AtMAN3;
DE Flags: Precursor;
GN Name=MAN3; OrderedLocusNames=At3g10890; ORFNames=T7M13.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flowers, siliques and seeds.
CC {ECO:0000269|PubMed:16897088}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; AC011708; AAF19560.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74967.1; -; Genomic_DNA.
DR RefSeq; NP_187700.1; NM_111926.2.
DR AlphaFoldDB; Q9SG94; -.
DR SMR; Q9SG94; -.
DR STRING; 3702.AT3G10890.1; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; Q9SG94; -.
DR PRIDE; Q9SG94; -.
DR ProteomicsDB; 238234; -.
DR EnsemblPlants; AT3G10890.1; AT3G10890.1; AT3G10890.
DR GeneID; 820259; -.
DR Gramene; AT3G10890.1; AT3G10890.1; AT3G10890.
DR KEGG; ath:AT3G10890; -.
DR Araport; AT3G10890; -.
DR TAIR; locus:2103262; AT3G10890.
DR eggNOG; ENOG502QS4Q; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q9SG94; -.
DR OMA; HVKTMVN; -.
DR OrthoDB; 1003648at2759; -.
DR PhylomeDB; Q9SG94; -.
DR BioCyc; ARA:AT3G10890-MON; -.
DR PRO; PR:Q9SG94; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SG94; baseline and differential.
DR Genevisible; Q9SG94; AT.
DR GO; GO:0048046; C:apoplast; IDA:TAIR.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IDA:TAIR.
DR GO; GO:0071585; P:detoxification of cadmium ion; IMP:TAIR.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR GO; GO:0046686; P:response to cadmium ion; IMP:TAIR.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..414
FT /note="Mannan endo-1,4-beta-mannosidase 3"
FT /id="PRO_0000277476"
FT ACT_SITE 203
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 323
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 87
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 202
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 283
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 365
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT CARBOHYD 17
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 386
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 414 AA; 46462 MW; BBACD11830EB2609 CRC64;
MKCLCFVVLL AILIAQNSSD LGVKSASSDG FVSRKGVQFI LNGKPFYANG FNAYWLAYEA
TDSTTRFKIT YVFQNATIHD LTIVRTWGFR DGGYRALQIA PGVYDEKTFQ GLDFAIAEAK
RLGIKMIITF VNNYSDFGGR KQYVDWAKNT GQNVSSDDDF YTNPLVKQYY KNHVKTMVNR
VNTFTKVEYK DEPTIMGWEL MNEPQCRADP SGKTLTAWMN EMALYVKSVD SKHLLSTGLE
GFYGDSSPQR KTSLNPVAAN VLGTDFIANH KLDAIDFASI HSYPDLWFPN LDEKSRLNLL
RKWLECHLED AQNILKKPLI LGEFGKPTNT PGYTQAQRDA VFNATFDTIY ESAEKGGPAA
GALFWHVISD GMNNFKDPLS IVLSENSTTV NIITEESRKL GLIRGKGKLS KTKI
