MAN4_ARATH
ID MAN4_ARATH Reviewed; 408 AA.
AC Q9SG95;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative mannan endo-1,4-beta-mannosidase 4;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 4;
DE AltName: Full=Endo-beta-1,4-mannanase 4;
DE Short=AtMAN4;
DE Flags: Precursor;
GN Name=MAN4; OrderedLocusNames=At3g10900; ORFNames=T7M13.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; AC011708; AAF19559.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74968.1; -; Genomic_DNA.
DR RefSeq; NP_187701.1; NM_111927.1.
DR AlphaFoldDB; Q9SG95; -.
DR SMR; Q9SG95; -.
DR STRING; 3702.AT3G10900.1; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; Q9SG95; -.
DR PRIDE; Q9SG95; -.
DR ProteomicsDB; 238553; -.
DR EnsemblPlants; AT3G10900.1; AT3G10900.1; AT3G10900.
DR GeneID; 820260; -.
DR Gramene; AT3G10900.1; AT3G10900.1; AT3G10900.
DR KEGG; ath:AT3G10900; -.
DR Araport; AT3G10900; -.
DR TAIR; locus:2103197; AT3G10900.
DR eggNOG; ENOG502QS4Q; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q9SG95; -.
DR OMA; DWINEMA; -.
DR OrthoDB; 1003648at2759; -.
DR PhylomeDB; Q9SG95; -.
DR BioCyc; ARA:AT3G10900-MON; -.
DR PRO; PR:Q9SG95; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SG95; baseline.
DR Genevisible; Q9SG95; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..408
FT /note="Putative mannan endo-1,4-beta-mannosidase 4"
FT /id="PRO_0000277477"
FT ACT_SITE 202
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 322
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 85
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 201
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 364
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 408 AA; 45376 MW; 86C48FA7D311BA15 CRC64;
MKCLCFIVLL AIVIAQSYVG VEAAPSDGFV SRNGVQFILN GKPFYANGFN AYWLAYEATD
PTTRFKITNV FQNATSLGLT IARTWGFRDG AIYRALQTAP GSYDEQTFQG LDFVIAEAKR
IGIKLIILLV NNWDDYGGKK QYVDWARSKG EVVSSNDDFY RNPVIKDFYK NHVKTVLNRV
NTFTKVAYKD EPAIMAWQLM NEPRCGVDKS GKTLMDWINE MAPFVKSVDP NHLLSTGHEG
FYGDSSPERK NSLNPVSANT VGADFIANHN IDAIDFASMH CGSDLWFQRL DQNSRLAFIK
RWLEGHIEDA QNILKKPVIL AEFGLGSDTP RYTLANRDGV FTTTYDIIYA SAQKGGSAAG
ALFWEVISEG MSNFAGPSSI ILSDKSSTVN IISEHARKMG LLGETTRK
