MAN4_ORYSJ
ID MAN4_ORYSJ Reviewed; 461 AA.
AC Q10B67; A0A0P0W5B7; Q10B68; Q8SAY2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Mannan endo-1,4-beta-mannosidase 4;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 4;
DE AltName: Full=Endo-beta-1,4-mannanase 4;
DE AltName: Full=OsMAN4;
GN Name=MAN4; OrderedLocusNames=Os03g0828500, LOC_Os03g61280;
GN ORFNames=OSJNBa0010E04.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q10B67-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q10B67-2; Sequence=VSP_023021;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16897088}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; AC096687; AAL79758.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99676.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99677.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13691.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS87176.1; -; Genomic_DNA.
DR EMBL; AK106915; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015628087.1; XM_015772601.1. [Q10B67-1]
DR RefSeq; XP_015628088.1; XM_015772602.1. [Q10B67-2]
DR AlphaFoldDB; Q10B67; -.
DR SMR; Q10B67; -.
DR STRING; 4530.OS03T0828500-01; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; Q10B67; -.
DR PRIDE; Q10B67; -.
DR EnsemblPlants; Os03t0828500-01; Os03t0828500-01; Os03g0828500. [Q10B67-1]
DR GeneID; 4334653; -.
DR Gramene; Os03t0828500-01; Os03t0828500-01; Os03g0828500. [Q10B67-1]
DR KEGG; osa:4334653; -.
DR eggNOG; ENOG502QS4Q; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q10B67; -.
DR OMA; ILGWELM; -.
DR OrthoDB; 1003648at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10B67; OS.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycosidase; Hydrolase; Reference proteome.
FT CHAIN 1..461
FT /note="Mannan endo-1,4-beta-mannosidase 4"
FT /id="PRO_0000277485"
FT ACT_SITE 196
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 314
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 80
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 195
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 274
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 357
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 364
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT VAR_SEQ 1..22
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_023021"
FT CONFLICT 421
FT /note="G -> S (in Ref. 5; AK106915)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 461 AA; 50739 MW; 872068A0D8DDD08F CRC64;
MSSCALVQRP PPWRAAVAPG DGMVAVDGTQ FVVDCGRTIF FSGFNAYWLM MMAADPALRG
AVATAFQQAS AHGLNLARTW AFSDGGDQPL QSSPGVYNET MFQGLDFVIA EARRHGIYLL
LCLTNNFDNF GGKRQYVRWA GDAGHNLTSD DDFFTSTIVK SYFKNHVKTV LTRVNTLTGV
AYKDDPTIFA WELMNEPRCY ADPTGAMVQA WVEEMAPYVK SVDGRHLVTP GLEGFYGAGE
HESKELNPWG IYYGTNYVAT HRTAAVDFAT IHLYPDVWLW GSSADEQATF FRNWTRSHID
ATAAYLGMPL LVTEYGKFLW KEVGANKAQR NYFLDLVLDA IYASASRGGP LVGGAFWQLL
LDGDIVAGMD SLRDGYEIIL AEDSRAASII GEHSEQLAAL NGQDADVLCR RASSHRRTRL
GNSLSCGGGD TLELLLRMVL ACFVSLSRSI SSFIVQNFIL L
