MAN5_ORYSJ
ID MAN5_ORYSJ Reviewed; 491 AA.
AC Q5W6G0; A0A0N7KKI9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Putative mannan endo-1,4-beta-mannosidase 5;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 5;
DE AltName: Full=Endo-beta-1,4-mannanase 5;
DE AltName: Full=OsMAN5;
DE Flags: Precursor;
GN Name=MAN5; OrderedLocusNames=Os05g0319100, LOC_Os05g25480;
GN ORFNames=OSJNBb0006B22.2, OSJNBb0059K16.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP GENE FAMILY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expression not detected.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAV44080.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAV44120.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC134344; AAV44120.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC136224; AAV44080.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014961; BAS93345.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5W6G0; -.
DR SMR; Q5W6G0; -.
DR STRING; 4530.OS05T0319100-00; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; Q5W6G0; -.
DR PRIDE; Q5W6G0; -.
DR EnsemblPlants; Os05t0319100-00; Os05t0319100-00; Os05g0319100.
DR Gramene; Os05t0319100-00; Os05t0319100-00; Os05g0319100.
DR eggNOG; ENOG502QU0Z; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q5W6G0; -.
DR OMA; TPQPFRM; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q5W6G0; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..491
FT /note="Putative mannan endo-1,4-beta-mannosidase 5"
FT /id="PRO_0000277486"
FT ACT_SITE 249
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 372
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 134
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 248
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 330
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 416
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT CARBOHYD 385
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 471
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 491 AA; 53465 MW; 4494130235C34371 CRC64;
METSYREEEA RRKASLLHCI FFFLLGALAM AAAIAVLHES SYWEWRCNRL TDIVVDGDDG
DGPSSSEVVD GGGEWGMVRT RGAQFVVGGG RPFYVNGFNT YWLMVLAVDP STRGKVTEVF
RQAAAVGLTV CRTWAFNDGG WRALQKSPGV YDEEVFKALD FVVSEARKHK IRLILPLINN
WDDYGGKAQY VRWAQAAAAG AGADAFFSDE TVRGYFKSHV TAVLTRVNAY TGVAYRDDPT
IMAWELMNEP RCASDPTGDT LQAWIAEMAF HVKSVDPAHL LGVGAEGFYG PSSPPARLRV
NPNADVALAG ADFVRNHRVL GVDFASVHVY PDTWLPAGAT KEAQLRFATS WVEAHIADAE
GALGGMPVLF AEFGVSTRGA RAAFNATSRD AFIEAVYGAM LRSTRRGGGG AGALLWQVFP
EGTDYMDDGY AVVLPRAAAT AGIVAAHSRR LQSFNSRCAW SCRWGCNKRD NDTAETTTAE
ADVDVSFHHE L
