MAN5_SOLLC
ID MAN5_SOLLC Reviewed; 414 AA.
AC Q6YM50;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Mannan endo-1,4-beta-mannosidase 5;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 5;
DE AltName: Full=Endo-beta-1,4-mannanase 5;
DE AltName: Full=LeMAN5;
DE Flags: Precursor;
GN Name=MAN5;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Moneymaker;
RX PubMed=14976239; DOI=10.1104/pp.103.035998;
RA Filichkin S.A., Leonard J.M., Monteros A., Liu P.-P., Nonogaki H.;
RT "A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with
RT anther and pollen development.";
RL Plant Physiol. 134:1080-1087(2004).
CC -!- FUNCTION: May be involved in weakening of anther wall during pollen
CC development.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in anthers and pollen of unopened bud
CC and opening flowers, but not in fully opened flowers.
CC {ECO:0000269|PubMed:14976239}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; AY102168; AAM26920.1; -; Genomic_DNA.
DR RefSeq; NP_001234473.1; NM_001247544.2.
DR AlphaFoldDB; Q6YM50; -.
DR SMR; Q6YM50; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR GeneID; 544170; -.
DR KEGG; sly:544170; -.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q6YM50; -.
DR OrthoDB; 1003648at2759; -.
DR PhylomeDB; Q6YM50; -.
DR BRENDA; 3.2.1.78; 3101.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; Q6YM50; baseline.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..414
FT /note="Mannan endo-1,4-beta-mannosidase 5"
FT /id="PRO_5000088873"
FT ACT_SITE 211
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 328
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 95
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 210
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 288
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 370
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
SQ SEQUENCE 414 AA; 46835 MW; 2ED692448896046E CRC64;
MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN GSPFLFNGFN
SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG GDRALQISPG VYDERVFQGL
DFVISEAKKY GIRLILSFVN NYNDFGGKAQ YVQWARNAGA QINGDDDFYT NYITKNYYKN
HIKKVVTRFN TITGMTYKDD STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK
HLLEIGMEGF YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA
QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI YRNIYSLAKD
GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS QSHAMTALAH LVKI
