MAN6_ORYSJ
ID MAN6_ORYSJ Reviewed; 440 AA.
AC Q0DCM5; A0A0N7KM01; Q5Z7X8;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Mannan endo-1,4-beta-mannosidase 6;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 6;
DE AltName: Full=Endo-beta-1,4-mannanase 6;
DE AltName: Full=OsMAN6;
DE Flags: Precursor;
GN Name=MAN6; OrderedLocusNames=Os06g0311600, LOC_Os06g20620;
GN ORFNames=OSJNBb0005N06.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RA Kikuchi S.;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in stems and leaves, and at lower levels
CC in roots and seeds. {ECO:0000269|PubMed:16897088}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF19398.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004583; BAD61770.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19398.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; BAS97441.1; -; Genomic_DNA.
DR EMBL; AK242683; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015640965.1; XM_015785479.1.
DR AlphaFoldDB; Q0DCM5; -.
DR SMR; Q0DCM5; -.
DR STRING; 4530.OS06T0311600-01; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; Q0DCM5; -.
DR PRIDE; Q0DCM5; -.
DR EnsemblPlants; Os06t0311600-01; Os06t0311600-01; Os06g0311600.
DR GeneID; 4340863; -.
DR Gramene; Os06t0311600-01; Os06t0311600-01; Os06g0311600.
DR KEGG; osa:4340863; -.
DR eggNOG; ENOG502QVVQ; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; Q0DCM5; -.
DR OMA; PHSWIKG; -.
DR OrthoDB; 1003648at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q0DCM5; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..440
FT /note="Mannan endo-1,4-beta-mannosidase 6"
FT /id="PRO_0000277487"
FT ACT_SITE 224
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 344
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 223
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 304
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 388
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 330
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 356
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 401
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 418
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 354
FT /note="N -> S (in Ref. 4; AK242683)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 440 AA; 49455 MW; 244DD2B94365FAB3 CRC64;
MRQERRLYSL LGLLLLLAVV YLTWFPTTHD GGGGGGGGWV KLPVPWLQPR MPFAARRGTH
FVDADTGSPL YVNGWNSYWL LPARSPALAA EMLRRGRRMG LSVCRTWAFS DGGPGALQIS
PGRFSEAVFQ VLDYVIYEAR RNHIRLILCL VNNLDNLGGK AQYVQWAQAA GANMTNSTDS
FYSHPTIKRY YKDYVKAILT RRNSYSRIRY SDEPAIFAWE LMNEPRCVSN SSGPYLQAWI
AEMAAYVKSL DTNHLVTVGT EGFYGPGIAE RLGVNPGEWA ASLCSDFIQN SAVEHIDFAS
VHAYPDSWLP RASLEEKVRY LSNWVDSHLN DSEQILKKPV LFTEVGYLQH SDANSNSTVD
RDIILRIVYD KIYDSARKLQ AGSGALIWQL MVEGTHMYGD NFSVVARDRP STYSLITNQS
CRLQRLYGEG DPGWQCSIPP
