ID   MAN9_ORYSJ              Reviewed;         407 AA.
AC   Q6Z310;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Putative mannan endo-1,4-beta-mannosidase 9;
DE            EC=3.2.1.78;
DE   AltName: Full=Beta-mannanase 9;
DE   AltName: Full=Endo-beta-1,4-mannanase 9;
DE   AltName: Full=OsMANP;
DE   AltName: Full=OsMan9;
DE   Flags: Precursor;
GN   Name=MAN9; OrderedLocusNames=Os02g0766900, LOC_Os02g52800;
GN   ORFNames=OJ1004_A11.22, P0539D10.41;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA   Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT   "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL   Funct. Integr. Genomics 7:1-16(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expression not detected.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP004817; BAD17132.1; -; Genomic_DNA.
DR   EMBL; AP005287; BAD17320.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF10140.2; -; Genomic_DNA.
DR   EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q6Z310; -.
DR   SMR; Q6Z310; -.
DR   STRING; 4530.OS02T0766900-00; -.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   PaxDb; Q6Z310; -.
DR   PRIDE; Q6Z310; -.
DR   eggNOG; ENOG502QS4Q; Eukaryota.
DR   InParanoid; Q6Z310; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451; PTHR31451; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..407
FT                   /note="Putative mannan endo-1,4-beta-mannosidase 9"
FT                   /id="PRO_0000277490"
FT   ACT_SITE        209
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   ACT_SITE        324
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   BINDING         95
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         208
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         284
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         366
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
SQ   SEQUENCE   407 AA;  44739 MW;  1C0A0B9F113A417C CRC64;
     MGSKRRVILL PTLGVVVLAI AAAVLLHAGE AADVANGQFA RASGTRFTVG GRPFYSNGFN
     AYWLMYMASD PGDRSKAAGV LQQAASLRAT LVRTWAFSDG GYRPLQKSPG VYNEDMFMGL
     DFVIAEAKKR GLYLILSLVN NWDGFGGKKQ YVQWARDQGH NLGSDDDFFR SDVTKQFYKN
     HAVLTRVNKI TGVAYKDDPT IFAWELINEP RCQSDLSGKT LQAWVTEMAG YVKSVDPNHM
     VEIGLEGFYG ESMHKNFNPG YTVGTDFIAN NLVPAVDFAT IHSYPDQWVS GASSDEQVAF
     MRKWMADHIR DSAAVLRKPL LVTEFGWSAR SNGYTVAARD AYFRTVYDAV YASAREGGAC
     AGGLFWQVMA PGMESWTDGY EVVLERSKST ADVVAHQCAR IAGLSPA