MANA_ASPFN
ID MANA_ASPFN Reviewed; 386 AA.
AC B8NVK8;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 2.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Probable mannan endo-1,4-beta-mannosidase A;
DE EC=3.2.1.78;
DE AltName: Full=Endo-beta-1,4-mannanase A;
DE Flags: Precursor;
GN Name=manA; Synonyms=man1; ORFNames=AFLA_116950;
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC 167;
RX PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
CC -!- FUNCTION: Endo-1,4-mannanase, a crucial enzyme for depolymerization of
CC seed galactomannans and wood galactoglucomannans. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EED45466.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; EQ963485; EED45466.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_002384402.1; XM_002384361.1.
DR AlphaFoldDB; B8NVK8; -.
DR SMR; B8NVK8; -.
DR STRING; 5059.CADAFLAP00012267; -.
DR EnsemblFungi; EED45466; EED45466; AFLA_116950.
DR eggNOG; ENOG502QS4Q; Eukaryota.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046355; P:mannan catabolic process; IEA:UniProt.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; PTHR31451; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycoprotein; Glycosidase; Hydrolase; Secreted;
KW Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..386
FT /note="Probable mannan endo-1,4-beta-mannosidase A"
FT /id="PRO_0000393702"
FT ACT_SITE 208
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 316
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 95
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 207
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 283
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 346
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT CARBOHYD 336
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 386 AA; 42008 MW; F30ED415E9A34993 CRC64;
MKLNPSLLTA AGLVSAQLAS ALPQASSSTV SPSPSPSATP GSFVTTSGLN FVIDGKTGYF
AGSNSYWIGF QKNNDDVDLV FSHLQESGLK ILRVWGFNDV NQKPTDGSVY YHLLADGTAT
VNEGEDGLQR LDYVVSSAEK HGIKLIINFV NFWDDYGGIN AYVKAFGGSK EDFYTNDAMQ
AAYRAYIKAV ISRYSDSTAI FAWELANEPR CQGCETTVLY NWIESTSQYI KSLDSKHLVC
IGDEGFGLDT GSDGSYPYQY SEGSDFAKNL AIPTIDFGTF HLYPSSWGTT NDWGNGWVTS
HGAACKEAGK PCLFEEYGVT SDHCAVEKPW QNTALNTTAI SGDLYWQYGD QLSGGPSPDD
GNTFYYGTDD FKCLVTDHIA AINSRN