MANA_CANEN
ID MANA_CANEN Reviewed; 981 AA.
AC C0HJB3;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 26-NOV-2014, sequence version 1.
DT 03-AUG-2022, entry version 25.
DE RecName: Full=Alpha-mannosidase {ECO:0000303|PubMed:4973951};
DE Short=JBM {ECO:0000303|PubMed:24295789};
DE Short=Jbalpha-man {ECO:0000303|PubMed:21924285};
DE EC=3.2.1.24 {ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145};
DE Contains:
DE RecName: Full=Alpha-mannosidase, heavy subunit {ECO:0000303|PubMed:24221485};
DE Contains:
DE RecName: Full=Alpha-mannosidase, light subunit {ECO:0000303|PubMed:24221485};
OS Canavalia ensiformis (Jack bean) (Dolichos ensiformis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Canavalia.
OX NCBI_TaxID=3823 {ECO:0000303|PubMed:9442045};
RN [1]
RP PROTEIN SEQUENCE, PROTEOLYTIC PROCESSING, GLYCOSYLATION AT ASN-312 AND
RP ASN-446, DISULFIDE BONDS, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP IDENTIFICATION OF CLEAVAGE SITE.
RC TISSUE=Seed {ECO:0000303|PubMed:24295789};
RX PubMed=24295789; DOI=10.1093/glycob/cwt106;
RA Gnanesh Kumar B.S., Pohlentz G., Schulte M., Mormann M., Siva Kumar N.;
RT "Jack bean alpha-mannosidase: amino acid sequencing and N-glycosylation
RT analysis of a valuable glycomics tool.";
RL Glycobiology 24:252-261(2014).
RN [2]
RP PROTEIN SEQUENCE OF 135-154, ACTIVITY REGULATION, MASS SPECTROMETRY, ACTIVE
RP SITE, AND PROTEOLYTIC PROCESSING.
RX PubMed=9442045; DOI=10.1074/jbc.273.4.2067;
RA Howard S., He S., Withers S.G.;
RT "Identification of the active site nucleophile in jack bean alpha-
RT mannosidase using 5-fluoro-beta-L-gulosyl fluoride.";
RL J. Biol. Chem. 273:2067-2072(1998).
RN [3] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RX PubMed=12325362; DOI=10.1016/s0021-9258(18)99383-x;
RA Li Y.T.;
RT "Studies on the glycosidases in jack bean meal. I. Isolation and properties
RT of alpha-mannosidase.";
RL J. Biol. Chem. 242:5474-5480(1967).
RN [4] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, COFACTOR, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=4973951; DOI=10.1042/bj1100663;
RA Snaith S.M., Levvy G.A.;
RT "Purification and properties of alpha-D-mannosidase from jack-bean meal.";
RL Biochem. J. 110:663-670(1968).
RN [5] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND COFACTOR.
RX PubMed=1156387; DOI=10.1042/bj1470083;
RA Snaith S.M.;
RT "Characterization of jack-bean alpha-D-mannosidase as a zinc
RT metalloenzyme.";
RL Biochem. J. 147:83-90(1975).
RN [6] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX PubMed=5145; DOI=10.1016/0005-2744(76)90334-x;
RA Sheperd V., Montgomery R.;
RT "Alpha-D-Mannosidase. Preparation and properties of free and insolubilized
RT enzyme.";
RL Biochim. Biophys. Acta 429:884-894(1976).
RN [7] {ECO:0000305}
RP PROTEOLYTIC CLEAVAGE, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=24221485; DOI=10.1007/bf00394781;
RA Faye L., Greenwood J.S., Herman E.M., Sturm A., Chrispeels M.J.;
RT "Transport and posttranslational processing of the vacuolar enzyme alpha-
RT mannosidase in jack-bean cotyledons.";
RL Planta 174:271-282(1988).
RN [8] {ECO:0000305}
RP ACTIVITY REGULATION.
RX PubMed=15974577; DOI=10.1021/jm0409019;
RA Fiaux H., Popowycz F., Favre S., Schuetz C., Vogel P., Gerber-Lemaire S.,
RA Juillerat-Jeanneret L.;
RT "Functionalized pyrrolidines inhibit alpha-mannosidase activity and growth
RT of human glioblastoma and melanoma cells.";
RL J. Med. Chem. 48:4237-4246(2005).
RN [9] {ECO:0000305}
RP COFACTOR, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND
RP PRESENCE OF DISULFIDE BONDS.
RX PubMed=21924285; DOI=10.1016/j.ijbiomac.2011.08.033;
RA Kumar A., Gaikwad S.M.;
RT "Jack bean alpha-mannosidase (Jbalpha-man): tolerance to alkali, chelating
RT and reducing agents and energetics of catalysis and inhibition.";
RL Int. J. Biol. Macromol. 49:1066-1071(2011).
CC -!- FUNCTION: Liberates mannose from p-nitrophenyl-alpha-D-mannoside
CC (PubMed:12325362, PubMed:4973951, PubMed:1156387). Liberates mannose
CC from further alpha-D-mannosides including methyl-, benzyl-alpha-D-
CC mannoside, 1-6-linked di-, tri- and tetrasaccharides of alpha-D-mannose
CC and mannosyl-rhamnose (PubMed:12325362). Liberates mannose from various
CC glycoproteins like ovalbumin and ovomucoid (PubMed:12325362,
CC PubMed:5145). Does not hydrolyze beta-D-mannosides (PubMed:12325362).
CC Has glycosyltransferase activity, forming disaccharides from mannose
CC and lyxose but not from glucose, galactose, ribose, xylose or arabinose
CC (PubMed:12325362). {ECO:0000269|PubMed:1156387,
CC ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:4973951,
CC ECO:0000269|PubMed:5145}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-mannose residues
CC in alpha-D-mannosides.; EC=3.2.1.24;
CC Evidence={ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:12325362,
CC ECO:0000269|PubMed:4973951, ECO:0000269|PubMed:5145};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:1156387, ECO:0000269|PubMed:21924285,
CC ECO:0000269|PubMed:4973951};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:Q29451};
CC -!- ACTIVITY REGULATION: Inhibited by 2,3,4,6-tetra-O-acetyl-5-fluoro-beta-
CC L-gulopyranosyl fluoride which acts as a slow substrate, doubling as a
CC competitive inhibitor as it forms a high steady state concentration of
CC glycosyl-enzyme intermediate that blocks the active site
CC (PubMed:9442045). Inhibited by 2,3,4,6-tetra-O-acetyl-5-fluoro-alpha-D-
CC mannopyranosyl fluoride which also acts as a slow substrate but no
CC intermediates accumulate (PubMed:9442045). Inhibited by EDTA
CC (PubMed:4973951, PubMed:1156387, PubMed:21924285). Inhibited by metal
CC ion Cu(2+) (PubMed:4973951, PubMed:1156387). Inhibited by metal ions
CC Fe(2+), Cd(2+) and Co(2+) (PubMed:4973951). Inhibited by metal ions
CC Ag(+) and Hg(2+) (PubMed:12325362, PubMed:4973951). Competitively
CC inhibited by mannono-1-4-lactone and mannono-1-5-lactone
CC (PubMed:12325362). Inhibited by swainsonine but not by 1-
CC desoxymannojirimycin (PubMed:21924285). Inhibited by pyrrolidine-3,4-
CC diol derivatives (PubMed:15974577). {ECO:0000269|PubMed:1156387,
CC ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:15974577,
CC ECO:0000269|PubMed:21924285, ECO:0000269|PubMed:4973951,
CC ECO:0000269|PubMed:9442045}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.5 mM for p-nitrophenyl-alpha-D-mannoside
CC {ECO:0000269|PubMed:12325362};
CC KM=31 mM for benzyl-alpha-D-mannoside {ECO:0000269|PubMed:12325362};
CC KM=0.12 M for methyl-alpha-D-mannoside {ECO:0000269|PubMed:12325362};
CC KM=10.52 uM for 4-methylumbellideryl-alpha-mannoside
CC {ECO:0000269|PubMed:21924285};
CC pH dependence:
CC Optimum pH is 4-5 (PubMed:12325362, PubMed:5145, PubMed:21924285).
CC Activity is stable between pH 4.0 and pH 7.0 in the presence of
CC Zn(2+) (PubMed:4973951). Activity is stable between pH 6.0 and pH
CC 8.5, decreases below pH 5.5 at 25 degrees Celsius (PubMed:12325362).
CC Activity is lost at pH 3 and lower, rapidly diminishes at pH 7-10 but
CC is higher and longest at pH 11-12 (PubMed:21924285).
CC {ECO:0000269|PubMed:21924285, ECO:0000269|PubMed:4973951,
CC ECO:0000269|PubMed:5145};
CC Temperature dependence:
CC Retains 50% activity after 5 min at 70 degrees Celsius
CC (PubMed:12325362). Retains full activity after 5 min at 70 degrees
CC Celsius but is completely inactive after 5 min at 80 degrees Celsius
CC (PubMed:5145). Retains 100%, 50% and <10% activity after 2 hours at
CC 40, 55 and 70 degrees Celsius, respectively (PubMed:21924285).
CC {ECO:0000269|PubMed:12325362, ECO:0000269|PubMed:21924285,
CC ECO:0000269|PubMed:5145};
CC -!- SUBUNIT: Dimer of dimers of heavy and light subunits.
CC {ECO:0000269|PubMed:21924285, ECO:0000303|PubMed:5145}.
CC -!- SUBCELLULAR LOCATION: Protein storage vacuole
CC {ECO:0000269|PubMed:24221485}.
CC -!- DEVELOPMENTAL STAGE: Expressed from very early on in developing
CC cotyledons. {ECO:0000269|PubMed:24221485}.
CC -!- PTM: Produced as a precursor which is then proteolytically cleaved into
CC a 66kD heavy subunit and a 44kD light subunit. Cleavage probably occurs
CC in protein bodies/protein storage vacuoles.
CC {ECO:0000269|PubMed:24221485, ECO:0000269|PubMed:24295789,
CC ECO:0000269|PubMed:9442045, ECO:0000303|PubMed:24221485}.
CC -!- MASS SPECTROMETRY: [Alpha-mannosidase, heavy subunit]: Mass=66533;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:9442045};
CC -!- MASS SPECTROMETRY: [Alpha-mannosidase, light subunit]: Mass=43846;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:9442045};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family. {ECO:0000255}.
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DR PDB; 6B9O; X-ray; 1.84 A; A/B=1-981.
DR PDB; 6B9P; X-ray; 2.00 A; A/B=1-981.
DR PDBsum; 6B9O; -.
DR PDBsum; 6B9P; -.
DR SMR; C0HJB3; -.
DR BindingDB; C0HJB3; -.
DR ChEMBL; CHEMBL3392945; -.
DR CAZy; GH38; Glycoside Hydrolase Family 38.
DR GlyConnect; 44; 3 N-Linked glycans.
DR iPTMnet; C0HJB3; -.
DR GO; GO:0000326; C:protein storage vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0004559; F:alpha-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
DR Gene3D; 1.20.1270.50; -; 2.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 3.20.110.10; -; 1.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR041147; GH38_C.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR011682; Glyco_hydro_38_C.
DR InterPro; IPR015341; Glyco_hydro_38_cen.
DR InterPro; IPR037094; Glyco_hydro_38_cen_sf.
DR InterPro; IPR000602; Glyco_hydro_38_N.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR013780; Glyco_hydro_b.
DR Pfam; PF09261; Alpha-mann_mid; 1.
DR Pfam; PF17677; Glyco_hydro38C2; 1.
DR Pfam; PF07748; Glyco_hydro_38C; 1.
DR Pfam; PF01074; Glyco_hydro_38N; 1.
DR SMART; SM00872; Alpha-mann_mid; 1.
DR SUPFAM; SSF74650; SSF74650; 1.
DR SUPFAM; SSF88688; SSF88688; 1.
DR SUPFAM; SSF88713; SSF88713; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Glycosidase; Hydrolase; Metal-binding; Vacuole; Zinc.
FT CHAIN 1..562
FT /note="Alpha-mannosidase, heavy subunit"
FT /evidence="ECO:0000269|PubMed:24295789"
FT /id="PRO_0000431307"
FT CHAIN 563..981
FT /note="Alpha-mannosidase, light subunit"
FT /evidence="ECO:0000269|PubMed:24295789"
FT /id="PRO_0000431308"
FT ACT_SITE 145
FT /note="Nucleophile"
FT /evidence="ECO:0000269|PubMed:9442045"
FT BINDING 23
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q29451"
FT BINDING 25
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q29451"
FT BINDING 145
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q29451"
FT BINDING 386
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q29451"
FT SITE 562..563
FT /note="Cleavage; to produce subunits"
FT /evidence="ECO:0000269|PubMed:24295789"
FT CARBOHYD 312
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:24295789"
FT CARBOHYD 446
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:24295789"
FT DISULFID 422..432
FT /evidence="ECO:0000269|PubMed:24295789"
FT DISULFID 442..450
FT /evidence="ECO:0000269|PubMed:24295789"
FT DISULFID 800..807
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 2
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 13
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 14
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 18
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 29
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 30
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 34
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 43
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 44
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 52
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 59
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 60
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 66
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 80
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 82
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 87
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 89
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 92
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 93
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 95
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 98
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 99
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 117
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 120
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 125
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 130
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 131
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 133
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 156
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 157
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 161
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 171
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 174
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 178
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 180
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 183
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 185
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 193
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 200
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 201
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 227
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 228
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 233
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 243
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 246
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 250
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 252
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 254
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 255
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 261
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 270
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 272
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 279
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 280
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 283
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 284
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 285
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 290
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 297
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 303
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 308
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 313
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 317
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 318
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 319
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 350
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 355
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 366
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 367
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 370
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 376
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 380
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 382
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 384
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 394
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 395
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 403
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 405
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 407
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 411
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 420
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 423
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 426
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 427
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 431
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 441
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 444
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 447
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 457
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 461
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 463
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 471
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 478
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 481
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 488
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 491
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 497
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 498
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 501
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 512
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 518
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 534
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 536
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 542
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 549
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 555
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 561
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 563
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 565
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 566
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 570
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 572
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 577
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 578
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 584
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 587
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 588
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 589
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 595
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 600
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 602
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 603
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 604
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 607
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 620
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 626
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 632
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 651
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 657
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 658
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 663
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 665
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 669
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 671
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 673
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 678
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 682
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 685
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 693
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 696
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 707
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 719
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 720
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 731
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 735
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 744
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 746
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 748
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 751
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 754
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 760
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 770
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 771
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 777
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 779
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 784
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 795
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 798
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 803
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 810
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 818
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 820
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 822
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 835
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 837
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 842
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 843
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 847
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 854
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 860
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 870
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 876
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 877
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 879
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 882
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 886
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 888
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 889
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 891
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 894
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 906
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 908
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 911
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 912
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 913
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 914
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 918
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 919
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 920
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 923
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 928
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 932
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 934
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 938
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 939
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 941
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 950
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 953
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 969
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 974
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 978
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 979
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:24295789"
FT UNSURE 980
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:24295789"
FT STRAND 15..20
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 32..37
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 41..43
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 48..61
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 72..79
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 84..95
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 98..103
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 116..134
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 140..142
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 144..149
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 151..155
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 156..158
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 159..161
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 173..181
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 185..190
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 196..199
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 200..205
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 238..253
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 256..265
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 274..291
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 293..298
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 301..310
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 317..320
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 326..328
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 331..333
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 335..337
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 341..363
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 376..383
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 386..390
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 395..423
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 436..438
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 445..447
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 451..454
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 462..469
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 471..473
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 475..484
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 489..491
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 500..504
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 507..521
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 530..539
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 543..550
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 574..581
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 583..585
FT /evidence="ECO:0007829|PDB:6B9P"
FT STRAND 588..593
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 594..597
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 598..611
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 613..615
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 618..621
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 642..647
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 650..660
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 663..670
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 674..682
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 693..701
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 708..713
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 718..722
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 727..729
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 738..740
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 742..751
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 756..760
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 765..768
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 774..782
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 788..790
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 812..821
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 823..825
FT /evidence="ECO:0007829|PDB:6B9P"
FT HELIX 826..838
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 842..848
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 850..856
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 859..864
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 874..881
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 886..893
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 901..903
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 907..910
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 911..914
FT /evidence="ECO:0007829|PDB:6B9O"
FT TURN 915..917
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 922..926
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 930..933
FT /evidence="ECO:0007829|PDB:6B9O"
FT HELIX 934..936
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 966..969
FT /evidence="ECO:0007829|PDB:6B9O"
FT STRAND 974..980
FT /evidence="ECO:0007829|PDB:6B9O"
SQ SEQUENCE 981 AA; 111024 MW; 58C01389BDDCC20A CRC64;
MKYNTGAGTV PEQLNVHLVP HSHDDVGWLK TVDQYYVGSE NYIQEACVEN VLDSVVMSLQ
RDPNRKFVFG EMAFFHRWWL EQTPETKELX XKLVKAGQLE FVNGGWCMHD EATTHYIDMI
DHTTLGHRFL QEQFNKIPRA GWQIDPFGHS AVQGYLLGAE LGFDSVHFAR IDYQDREKRK
GEKSLEVVWR GSKTFGSSAQ IFANAFPGHY GPPNGFNFEV RNNFVPLQDD PRLFDTNVEE
RVQNFLDAAL TQAKLTRTNH LMWTMGDDFQ YQYAESWFKQ MDKLLHHVNK DGRVNALYST
PSLYTEAKNA ANQTWPLKID DYFPYADGRN AYWTGFYTSR XXXXXXXXML SGYYLATRHS
GFFAGKKSTK YHAFDLADAL GIAQHHDAVS GTAKQHTTND YAKRLALGAS KAEAVVSSSL
ACLTSKQSAD QCSAPASAFS QCHLFNISYC PPTESSLPDD KSLVVVVYNP LGWSRNEIVR
IPVNDANLVV KDSSGNKLEV QYVEMDDVTA NLRSFYVKXX XXXXXXXXXX YWSLFKASVP
PLGWSTYFIS EATGKGTRNA LTLSQKGETL NIGPGDLKMS FSSLTGQLKR MYNSKTGVDL
PIQQNYLWYE SSEGDFSDYQ ASGAYLFRPN GQPPPHTVSR SSVTRVTRGP LVDEVHQKFN
SWISQVTRLY KDKDHAEIEF TIGPIPTDDG VGKEVITRMT STMATNKEFY TDSNGRDFLK
RVRDYREDWP LEVTQPVAGN YYPLNLGLYT KDEKSEFSVL VDRATGGASI KDGEVELMLH
RRTLRDDGRG VGEPLDEQVC MNKEYTCEGL TVRGNYYLSI HKPAGGSRWR RTTGQEIYSP
MLLAFTQENM ENWKSSHSTK AYAMDPNYSL PPSVALITLE ELDDGLVLLR LAHLYEPSED
AEYSTLTKVE LKKLFATQKL EELREVSLSA NQEKSEMKKM KWSVEGDNEQ EPQAVRGGPV
SNADFVVELG PMEIRTFLLQ F
