MANA_LABPU
ID MANA_LABPU Reviewed; 290 AA.
AC C0HJW7;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-DEC-2015, sequence version 1.
DT 25-MAY-2022, entry version 15.
DE RecName: Full=Alpha-mannosidase {ECO:0000303|PubMed:23422784};
DE EC=3.2.1.24 {ECO:0000269|PubMed:23422784};
DE Flags: Fragments;
OS Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Lablab.
OX NCBI_TaxID=35936 {ECO:0000303|PubMed:23422784};
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ACTIVITY
RP REGULATION, SUBUNIT, GLYCOSYLATION AT ASN-64, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Seed {ECO:0000303|PubMed:23422784};
RX PubMed=23422784; DOI=10.1016/j.pep.2013.02.004;
RA Gnanesh Kumar B.S., Pohlentz G., Mormann M., Siva Kumar N.;
RT "Characterization of alpha-mannosidase from Dolichos lablab seeds: partial
RT amino acid sequencing and N-glycan analysis.";
RL Protein Expr. Purif. 89:7-15(2013).
CC -!- FUNCTION: Liberates mannose from p-nitrophenyl-alpha-D-mannoside.
CC {ECO:0000269|PubMed:23422784}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-mannose residues
CC in alpha-D-mannosides.; EC=3.2.1.24;
CC Evidence={ECO:0000269|PubMed:23422784};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:23422784};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000305|PubMed:23422784};
CC -!- ACTIVITY REGULATION: Inhibited by swainsonine but not by 1-
CC desoxymannojirimycin. {ECO:0000269|PubMed:23422784}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.48 mM for p-nitrophenyl-alpha-D-mannopyranoside
CC {ECO:0000269|PubMed:23422784};
CC pH dependence:
CC Optimum pH is 5. {ECO:0000269|PubMed:23422784};
CC Temperature dependence:
CC Optimum temperature is 65 degrees Celsius. Activity decreases sharply
CC above 70 degrees Celsius. {ECO:0000269|PubMed:23422784};
CC -!- SUBUNIT: Dimer. {ECO:0000269|PubMed:23422784}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family.
CC {ECO:0000303|PubMed:23422784}.
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DR AlphaFoldDB; C0HJW7; -.
DR iPTMnet; C0HJW7; -.
DR GO; GO:0004559; F:alpha-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
DR Gene3D; 1.20.1270.50; -; 2.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR037094; Glyco_hydro_38_cen_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR SUPFAM; SSF74650; SSF74650; 1.
DR SUPFAM; SSF88688; SSF88688; 1.
DR SUPFAM; SSF88713; SSF88713; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW Metal-binding; Zinc.
FT CHAIN 1..290
FT /note="Alpha-mannosidase"
FT /id="PRO_0000435002"
FT ACT_SITE 17
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:C0HJB3"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 1
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 6
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 7
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 10
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 15
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 16
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 25
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 35
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 38
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 43
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 49
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 60
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 62
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 65
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 71
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 77
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 82
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 91
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 92
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 112
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 116
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 118
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 120
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 130
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 131
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 133
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 136
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 141
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 146
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 150
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 155
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 157
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 160
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 161
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 162
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 164
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 165
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 166
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 172
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 180
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 183
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 189
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 195
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 207
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 208
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 211
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 216
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 221
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 222
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 223
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 227
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 229
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 230
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 235
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 240
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 245
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 246
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 249
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 250
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 252
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 256
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 259
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 264
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 271
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 273
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 277
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 278
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 279
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 284
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT UNSURE 287
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:23422784"
FT NON_CONS 14..15
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 27..28
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 67..68
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 102..103
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 130..131
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 148..149
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 166..167
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 183..184
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 188..189
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 202..203
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 232..233
FT /evidence="ECO:0000303|PubMed:23422784"
FT NON_CONS 252..253
FT /evidence="ECO:0000303|PubMed:23422784"
SQ SEQUENCE 290 AA; 32157 MW; 1B58C33A8666E361 CRC64;
LVSSGQLEFI DGGVQIDPFG HSAVQAYFEV NDNSLPVQDN VELFDYNVQE RVNDFVAAAL
SQANITRVNA LYSTPSIYTD AKYATNEYWP LKTDDFFPYA DRFNSGPNTD SLADALAIAQ
HHDAVTGTEK LAIGYQEAEE LVSSSLACVQ DSDGLEIESQ LLPQQKVSVP PLGFSTYTVL
TAKYDETGQA SGAYLFRPDG TWHGNAKLTV LDGPVLDEVH QQINPWIYQI TRSVLVDRPL
GGSSLQDGQI ELYYRIDPLG EGAKWRRSFG QEIYSPLLLA FAEQDDQDEW
