MANBB_ASPFU
ID MANBB_ASPFU Reviewed; 845 AA.
AC Q4WAH4;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Beta-mannosidase B;
DE EC=3.2.1.25;
DE AltName: Full=Mannanase B;
DE Short=Mannase B;
GN Name=mndB; ORFNames=AFUA_7G01320;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Exoglycosidase that cleaves the single beta-linked mannose
CC residue from the non-reducing end of beta-mannosidic oligosaccharides
CC of various complexity and length. Prefers mannobiose over mannotriose
CC and has no activity against polymeric mannan. Is also severely
CC restricted by galactosyl substitutions at the +1 subsite (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC -!- MISCELLANEOUS: In contrast to clade A beta-mannosidases, which are
CC likely secreted, clade B proteins appear to be intracellular.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000305}.
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DR EMBL; AAHF01000015; EAL84762.1; -; Genomic_DNA.
DR RefSeq; XP_746800.1; XM_741707.1.
DR AlphaFoldDB; Q4WAH4; -.
DR SMR; Q4WAH4; -.
DR STRING; 746128.CADAFUBP00008544; -.
DR EnsemblFungi; EAL84762; EAL84762; AFUA_7G01320.
DR GeneID; 3504183; -.
DR KEGG; afm:AFUA_7G01320; -.
DR VEuPathDB; FungiDB:Afu7g01320; -.
DR eggNOG; KOG2230; Eukaryota.
DR HOGENOM; CLU_005015_1_0_1; -.
DR InParanoid; Q4WAH4; -.
DR OMA; MFANFDY; -.
DR OrthoDB; 517710at2759; -.
DR UniPathway; UPA00280; -.
DR Proteomes; UP000002530; Chromosome 7.
DR GO; GO:0004567; F:beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0006516; P:glycoprotein catabolic process; IBA:GO_Central.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF49303; SSF49303; 2.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome.
FT CHAIN 1..845
FT /note="Beta-mannosidase B"
FT /id="PRO_0000394654"
FT ACT_SITE 432
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 717
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 723
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 845 AA; 96998 MW; B6EA86796A8C33DE CRC64;
MSKLQQFPLS KGWSFRDNEA TSEDAWMPVP VVPSVVHQDL QANNKLKDPY IGFNELEARW
VNEKSWTYKT VFQKPAAPAG SCIVLAFDGL DTFAKVKLDG NVILENDNMF LARRVDVTKA
LEAEGDHVLE IDFDCAFLRA KELRKQDPRH NWASFNGDPS RLSVRKAQYH WGWDWGPVLM
TAGIWREVRL EVYSARVADL WTEVQLASDH QSAQVTAFVE VESVHSGSHR ACFTLSLHGQ
EITREEIGVT ENGTAKATFD VKEPSLWWPH GYGDATLYEV SVSLVKEQEE LHRVSKKFGI
RTAEVIQRPD KHGKSFFFRV NGVDIFCGGS CWIPADNLLP SITAERYRKW IELMVHGRQV
MIRVWGGGIY EDNSFYDACD ELGVLVWQDF MFGCGNYPTW PNLLESIRKE SVYNVRRLRH
HPSIVIWVGN NEDYQVQEQA GLTYNYEDKD PENWLKTDFP ARYIYEKLLP EVVQEYSPGT
FYHPGSPWGD GKTTSDPTVG DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI
DYFVENEADK YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETHIYL TQVVQAETMM
FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA RVLNPIAVGV
RREHHDWSVT HAQPPKTSKF ELWVASSRQQ EIQGTVELRF LSVNTGLEVR ERIVHENVSI
VPNGTTNLIV DGLIDHTVHS EPHVLAARIW VDGQLVARDV DWPQPFKYLD LSDRGLEVKK
ISESEDEQTL LISTKKPVKC LVFEEREGVR ISDSAMDIVP GDDQRVTIKG LKPGDAPLKY
KFLGQ
