ID   MANC7_ECOLX             Reviewed;         464 AA.
AC   P37741;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Mannose-1-phosphate guanylyltransferase;
DE            EC=2.7.7.13;
DE   AltName: Full=GDP-mannose pyrophosphorylase;
DE            Short=GMP;
DE            Short=GMPP;
GN   Name=manC; Synonyms=rfbM;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O7:K1 / VW187;
RX   PubMed=7677991; DOI=10.1128/jb.175.1.148-158.1993;
RA   Marolda C.L., Valvano M.A.;
RT   "Identification, expression, and DNA sequence of the GDP-mannose
RT   biosynthesis genes encoded by the O7 rfb gene cluster of strain VW187
RT   (Escherichia coli O7:K1).";
RL   J. Bacteriol. 175:148-158(1993).
CC   -!- FUNCTION: Involved in GDP-mannose biosynthesis which serves as the
CC       activated sugar nucleotide precursor for mannose residues in cell
CC       surface polysaccharides. This enzyme participates in synthesis of the
CC       LPS O7 antigen.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-
CC         alpha-D-mannose; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:58409; EC=2.7.7.13;
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP
CC       route): step 1/1.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the mannose-6-phosphate isomerase type 2 family.
CC       {ECO:0000305}.
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DR   EMBL; AF125322; AAC27538.1; -; Genomic_DNA.
DR   PIR; C40630; C40630.
DR   RefSeq; WP_000097982.1; NZ_UIJD01000062.1.
DR   AlphaFoldDB; P37741; -.
DR   SMR; P37741; -.
DR   UniPathway; UPA00126; UER00930.
DR   UniPathway; UPA00281; -.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004475; F:mannose-1-phosphate guanylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009298; P:GDP-mannose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.120.10; -; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR006375; Man1P_GuaTrfase/Man6P_Isoase.
DR   InterPro; IPR001538; Man6P_isomerase-2_C.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF01050; MannoseP_isomer; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR01479; GMP_PMI; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Lipopolysaccharide biosynthesis; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..464
FT                   /note="Mannose-1-phosphate guanylyltransferase"
FT                   /id="PRO_0000194258"
SQ   SEQUENCE   464 AA;  52611 MW;  D1133D2D2B22E024 CRC64;
     MSSPLIPVIL SGGNGTRLWP LSREEYPKQF LKLTDSISML QSTISRLDSL NTSSPVVICN
     ELHRFIVAEQ LRHLNKLDNN IILEPSGRNT APAICIAALI LKMKHPNENP LMLVLPADHS
     VKKVKTFCNT IKSAIPFAEA GNLVSFGIKP THPETGYGYI QKGKVLSDSD IYEVSEVRTF
     VEKPNLKTAE SFIEKDEYYW NSGMYLFSVE RYLQELSLYR PDIVKVCQET VKNIHYDMDF
     IRLDDKIFRN CPQESIDYAV MEKTKDAVVA TMDIGWNDVG AWSSLWELGK KDSSGNVITG
     DIVCHETENS YIYTESGLVA TIGIQDLVII HTKDSLLVSR RDSVQNVKNI VQHLDLSGRK
     EHKEHREVFK SWGRCDSIDS SEKYHYQVKR ITVNPSENYR CNYIITVRNI GVVVMGIAKL
     TVAEEIKILK ENESVYIPAG IKHSLKILDN TTCVNRSLDR FLSC