ID   MANC_KLEPN              Reviewed;         471 AA.
AC   Q48462;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Mannose-1-phosphate guanylyltransferase;
DE            EC=2.7.7.13;
DE   AltName: Full=GDP-mannose pyrophosphorylase;
DE            Short=GMP;
DE            Short=GMPP;
DE   AltName: Full=ORF16;
GN   Name=manC; Synonyms=cpsB;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Chedid;
RX   PubMed=7896702; DOI=10.1128/jb.177.7.1788-1796.1995;
RA   Arakawa Y., Wacharotayankun R., Nagatsuka T., Ito H., Kato N., Ohta M.;
RT   "Genomic organization of the Klebsiella pneumoniae cps region responsible
RT   for serotype K2 capsular polysaccharide synthesis in the virulent strain
RT   Chedid.";
RL   J. Bacteriol. 177:1788-1796(1995).
CC   -!- FUNCTION: Involved in the biosynthesis of the K2 capsular
CC       polysaccharide biosynthesis.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-
CC         alpha-D-mannose; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:58409; EC=2.7.7.13;
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP
CC       route): step 1/1.
CC   -!- SIMILARITY: Belongs to the mannose-6-phosphate isomerase type 2 family.
CC       {ECO:0000305}.
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DR   EMBL; D21242; BAA04787.1; -; Genomic_DNA.
DR   PIR; E56146; E56146.
DR   RefSeq; WP_004180506.1; NZ_WXZY01000015.1.
DR   AlphaFoldDB; Q48462; -.
DR   SMR; Q48462; -.
DR   PRIDE; Q48462; -.
DR   UniPathway; UPA00126; UER00930.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004475; F:mannose-1-phosphate guanylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009298; P:GDP-mannose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR006375; Man1P_GuaTrfase/Man6P_Isoase.
DR   InterPro; IPR001538; Man6P_isomerase-2_C.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF01050; MannoseP_isomer; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR01479; GMP_PMI; 1.
PE   3: Inferred from homology;
KW   Capsule biogenesis/degradation; GTP-binding;
KW   Lipopolysaccharide biosynthesis; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..471
FT                   /note="Mannose-1-phosphate guanylyltransferase"
FT                   /id="PRO_0000194255"
SQ   SEQUENCE   471 AA;  52864 MW;  B3F46092D767D9E5 CRC64;
     MLLPVIMAGG TGSRLWPMSR ELYPKQFLRL FGQNSMLQET ITRLSGLEIH EPMVICNEEH
     RFLVAEQLRQ LNKLSNNIIL EPVGRNTAPA IALAALQATR HGDDPLMLVL AADHIINNQP
     VFHDAIRVAE QYADEGHLVT FGIVPNAPET GYGYIQRGVA LTDSAHTPYQ VARFVEKPDR
     ERAEAYLASG EYYWNSGMFM FRAKKYLSEL AKFRPDILEA CQAAVNAADN GSDFISIPHD
     IFCECPDESV DYAVMEKTAD AVVVGLDADW SDVGSWSALW EVSPKDEQGN VLSGDAWVHN
     SENCYINSDE KLVAAIGVEN LVIVSTKDAV LVMNRERSQD VKKAVEFLKQ NQRSEYKRHR
     EIYRPWGRCD VVVQTPRFNV NRITVKPGGA FSMQMHHHRA EHWVILAGTG QVTVNGKQFL
     LTENQSTFIP IGAEHSLENP GRIPLEVLEI QSGSYLGEDD IIRIKDQYGR C