MAOX_CHICK
ID MAOX_CHICK Reviewed; 15 AA.
AC Q92060;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=NADP-dependent malic enzyme;
DE Short=NADP-ME;
DE EC=1.1.1.40 {ECO:0000250|UniProtKB:P48163};
DE Flags: Fragment;
GN Name=ME1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8900406; DOI=10.1006/abbi.1996.0460;
RA Hodnett D.W., Fantozzi D.A., Thurmond D.C., Klautky S.A., Macphee K.G.,
RA Estrem S.T., Xu G., Goodridge A.G.;
RT "The chicken malic enzyme gene: structural organization and identification
RT of triiodothyronine response elements in the 5'-flanking DNA.";
RL Arch. Biochem. Biophys. 334:309-324(1996).
CC -!- FUNCTION: Catalyzes the oxidative decarboxylation of (S)-malate in the
CC presence of NADP (+) and divalent metal ions, and decarboxylation of
CC oxaloacetate. {ECO:0000250|UniProtKB:P48163}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NADP(+) = CO2 + NADPH + pyruvate;
CC Xref=Rhea:RHEA:18253, ChEBI:CHEBI:15361, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.40;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18254;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:18255;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + oxaloacetate = CO2 + pyruvate; Xref=Rhea:RHEA:15641,
CC ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:16526; EC=1.1.1.40;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15642;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:P48163};
CC Note=Divalent metal cations. Prefers magnesium or manganese.
CC {ECO:0000250|UniProtKB:P48163};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P48163}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P48163}.
CC -!- SIMILARITY: Belongs to the malic enzymes family. {ECO:0000305}.
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DR EMBL; U49693; AAA92721.1; -; Genomic_DNA.
DR eggNOG; KOG1257; Eukaryota.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR GO; GO:0004473; F:malate dehydrogenase (decarboxylating) (NADP+) activity; ISS:UniProtKB.
DR GO; GO:0030145; F:manganese ion binding; ISS:UniProtKB.
DR GO; GO:0008948; F:oxaloacetate decarboxylase activity; ISS:UniProtKB.
DR GO; GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
PE 3: Inferred from homology;
KW Cytoplasm; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..>15
FT /note="NADP-dependent malic enzyme"
FT /id="PRO_0000160197"
FT NON_TER 15
SQ SEQUENCE 15 AA; 1842 MW; CFEF180B2BA84C2B CRC64;
MKRGYEVVRD PHLNK
