ID   MAPCN_PIOVA             Reviewed;          89 AA.
AC   P86916;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Mapacalcine {ECO:0000303|PubMed:9187271};
OS   Pione vastifica (Boring sponge) (Cliona vastifica).
OC   Eukaryota; Metazoa; Porifera; Demospongiae; Heteroscleromorpha; Clionaida;
OC   Clionaidae; Pione.
OX   NCBI_TaxID=458513;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, DISULFIDE LINKS, AMIDATION AT GLN-89, MASS
RP   SPECTROMETRY, AND DIMERIZATION.
RX   PubMed=9187271; DOI=10.1124/mol.51.6.1042;
RA   Morel J.L., Drobecq H., Sautiere P., Tartar A., Mironneau J., Qar J.,
RA   Lavie J.L., Hugues M.;
RT   "Purification of a new dimeric protein from Cliona vastifica sponge, which
RT   specifically blocks a non-L-type calcium channel in mouse duodenal
RT   myocytes.";
RL   Mol. Pharmacol. 51:1042-1052(1997).
RN   [2] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=9512477; DOI=10.1042/bj3310177;
RA   Vidalenc P., Morel J.L., Mironneau J., Hugues M.;
RT   "125I-Labelled mapacalcine: a specific tool for a pharmacological approach
RT   to a receptor associated with a new calcium channel on mouse intestinal
RT   membranes.";
RL   Biochem. J. 331:177-184(1998).
RN   [3] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=10700606; DOI=10.1016/s0006-8993(99)02449-x;
RA   Mourre C., Mokrzycki N., Neuilly G., Richeux F., Creppy E.E., Hugues M.;
RT   "Distribution of mapacalcine receptors in the central nervous system of rat
RT   using the [125I]-labeled mapacalcine derivative.";
RL   Brain Res. 858:136-142(2000).
RN   [4] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=12914793; DOI=10.1016/s0006-291x(03)01432-3;
RA   Mourre C., Lazou B., Cambar J., Neuilly G., Hugues M.;
RT   "Characterization of mapacalcine-sensitive Ca(2+) channels in rat kidney.";
RL   Biochem. Biophys. Res. Commun. 308:602-607(2003).
RN   [5] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=12709054; DOI=10.1046/j.1432-1033.2003.03558.x;
RA   Crenesse D., Neuilly G., Gugenheim J., Ferre C., Hugues M.;
RT   "Mapacalcine specifically blocks hypoxia-induced calcium influx in rat
RT   hepatocytes.";
RL   Eur. J. Biochem. 270:1952-1957(2003).
CC   -!- FUNCTION: Blocks calcium currents via interaction with a yet unknown
CC       target protein. Has no effect on L-type, T-type, N-type or P/Q-type
CC       voltage-gated calcium channels (VGCC). Has no effect on voltage-gated
CC       potassium or chloride channels. Blocks non-L-type VGCC calcium currents
CC       in mouse duodenal myocytes (IC(50)=0.2 uM). Blocks calcium influx
CC       induced by hypoxia/reoxygenation in rat hepatocytes.
CC       {ECO:0000269|PubMed:10700606, ECO:0000269|PubMed:12709054,
CC       ECO:0000269|PubMed:12914793, ECO:0000269|PubMed:9187271,
CC       ECO:0000269|PubMed:9512477}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:9187271}.
CC   -!- PTM: Contains disulfide bonds which may also be involved in
CC       dimerization. {ECO:0000269|PubMed:9187271}.
CC   -!- MASS SPECTROMETRY: Mass=10182.1; Mass_error=0.9; Method=Electrospray;
CC       Note=Monomer.; Evidence={ECO:0000269|PubMed:9187271};
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DR   AlphaFoldDB; P86916; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Toxin.
FT   CHAIN           1..89
FT                   /note="Mapacalcine"
FT                   /id="PRO_0000410950"
FT   MOD_RES         89
FT                   /note="Glutamine amide"
FT                   /evidence="ECO:0000269|PubMed:9187271"
SQ   SEQUENCE   89 AA;  9542 MW;  0670A7EDF4708215 CRC64;
     ICNGQWTSVG SAGLYYTIKA DSMCVDIHYT DGFIQPSCQG LQVIGPCNRY QNGPRDFVAC
     QTSGGSGHPI CIQSTNGNIE LCANCYCPQ