ID   MARA_ECOLI              Reviewed;         127 AA.
AC   P0ACH5; P27246;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 2.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Multiple antibiotic resistance protein MarA;
GN   Name=marA; OrderedLocusNames=b1531, JW5249;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383113; DOI=10.1128/jb.175.5.1484-1492.1993;
RA   Cohen S.P., Haechler H., Levy S.B.;
RT   "Genetic and functional analysis of the multiple antibiotic resistance
RT   (mar) locus in Escherichia coli.";
RL   J. Bacteriol. 175:1484-1492(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8491710; DOI=10.1128/jb.175.10.2888-2894.1993;
RA   Gambino L., Gracheck S., Miller P.F.;
RT   "Overexpression of the MarA positive regulator is sufficient to confer
RT   multiple antibiotic resistance in Escherichia coli.";
RL   J. Bacteriol. 175:2888-2894(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   PubMed=9724717; DOI=10.1073/pnas.95.18.10413;
RA   Rhee S., Martin R.G., Rosner J.L., Davies D.R.;
RT   "A novel DNA-binding motif in MarA: the first structure for an AraC family
RT   transcriptional activator.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:10413-10418(1998).
CC   -!- FUNCTION: May be a transcriptional activator of genes involved in the
CC       multiple antibiotic resistance (Mar) phenotype. It can also activate
CC       genes such as sodA, zwf and micF.
CC   -!- SUBUNIT: Monomer.
CC   -!- INDUCTION: By various antibiotics or by sodium salicylate.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC16395.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M96235; AAC16395.1; ALT_INIT; Genomic_DNA.
DR   EMBL; L06966; AAA24113.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74604.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15221.2; -; Genomic_DNA.
DR   PIR; B47072; B47072.
DR   RefSeq; NP_416048.2; NC_000913.3.
DR   RefSeq; WP_000091199.1; NZ_STEB01000003.1.
DR   PDB; 1BL0; X-ray; 2.30 A; A=1-127.
DR   PDB; 1XS9; NMR; -; A=1-127.
DR   PDBsum; 1BL0; -.
DR   PDBsum; 1XS9; -.
DR   AlphaFoldDB; P0ACH5; -.
DR   SMR; P0ACH5; -.
DR   BioGRID; 4259110; 129.
DR   DIP; DIP-48136N; -.
DR   IntAct; P0ACH5; 1.
DR   STRING; 511145.b1531; -.
DR   PaxDb; P0ACH5; -.
DR   PRIDE; P0ACH5; -.
DR   EnsemblBacteria; AAC74604; AAC74604; b1531.
DR   EnsemblBacteria; BAA15221; BAA15221; BAA15221.
DR   GeneID; 66674615; -.
DR   GeneID; 947613; -.
DR   KEGG; ecj:JW5249; -.
DR   KEGG; eco:b1531; -.
DR   PATRIC; fig|1411691.4.peg.735; -.
DR   EchoBASE; EB1404; -.
DR   eggNOG; COG4977; Bacteria.
DR   HOGENOM; CLU_000445_81_14_6; -.
DR   InParanoid; P0ACH5; -.
DR   OMA; FKKHFNT; -.
DR   PhylomeDB; P0ACH5; -.
DR   BioCyc; EcoCyc:PD00365; -.
DR   EvolutionaryTrace; P0ACH5; -.
DR   PRO; PR:P0ACH5; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:EcoCyc.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   Pfam; PF12833; HTH_18; 1.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Antibiotic resistance; DNA-binding;
KW   Reference proteome; Repeat; Transcription; Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Multiple antibiotic resistance protein MarA"
FT                   /id="PRO_0000194531"
FT   DOMAIN          12..110
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        29..50
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        77..100
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   HELIX           8..19
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   TURN            20..23
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           29..34
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   STRAND          35..37
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           39..50
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           54..72
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           77..83
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           89..100
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   HELIX           104..108
FT                   /evidence="ECO:0007829|PDB:1BL0"
FT   STRAND          115..117
FT                   /evidence="ECO:0007829|PDB:1XS9"
SQ   SEQUENCE   127 AA;  15184 MW;  A547C8F1553A9196 CRC64;
     MSRRNTDAIT IHSILDWIED NLESPLSLEK VSERSGYSKW HLQRMFKKET GHSLGQYIRS
     RKMTEIAQKL KESNEPILYL AERYGFESQQ TLTRTFKNYF DVPPHKYRMT NMQGESRFLH
     PLNHYNS