MARF1_RAT
ID MARF1_RAT Reviewed; 1735 AA.
AC Q8VIG2; F1LNE9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Meiosis regulator and mRNA stability factor 1 {ECO:0000312|RGD:619770};
DE AltName: Full=Limkain-b1;
DE AltName: Full=Meiosis arrest female protein 1 {ECO:0000305};
GN Name=Marf1 {ECO:0000312|RGD:619770}; Synonyms=Lkap;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH LIMK2.
RA Miyamoto K., Nakamura T., Shirakawa K., Matsumoto K.;
RT "Molecular cloning and characterization of novel large protein, limkain b1,
RT which associates with the LIM-kinase 2.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
CC -!- FUNCTION: Essential regulator of oogenesis required for female meiotic
CC progression to repress transposable elements and preventing their
CC mobilization, which is essential for the germline integrity. Probably
CC acts via some RNA metabolic process, equivalent to the piRNA system in
CC males, which mediates the repression of transposable elements during
CC meiosis by forming complexes composed of RNAs and governs the
CC methylation and subsequent repression of transposons. Also required to
CC protect from DNA double-strand breaks (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with LIMK2. {ECO:0000269|Ref.1}.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000250}.
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DR EMBL; AB012133; BAB82432.1; -; mRNA.
DR RefSeq; NP_596912.1; NM_133421.2.
DR RefSeq; XP_003750827.1; XM_003750779.4.
DR AlphaFoldDB; Q8VIG2; -.
DR SMR; Q8VIG2; -.
DR IntAct; Q8VIG2; 2.
DR STRING; 10116.ENSRNOP00000068225; -.
DR iPTMnet; Q8VIG2; -.
DR PhosphoSitePlus; Q8VIG2; -.
DR jPOST; Q8VIG2; -.
DR PaxDb; Q8VIG2; -.
DR PRIDE; Q8VIG2; -.
DR Ensembl; ENSRNOT00000091520; ENSRNOP00000073018; ENSRNOG00000056248.
DR GeneID; 170946; -.
DR KEGG; rno:170946; -.
DR UCSC; RGD:619770; rat.
DR CTD; 9665; -.
DR RGD; 619770; Marf1.
DR eggNOG; ENOG502QUYZ; Eukaryota.
DR GeneTree; ENSGT00390000002393; -.
DR HOGENOM; CLU_002701_0_0_1; -.
DR InParanoid; Q8VIG2; -.
DR OMA; NNGHIME; -.
DR OrthoDB; 187067at2759; -.
DR TreeFam; TF329117; -.
DR PRO; PR:Q8VIG2; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000056248; Expressed in frontal cortex and 19 other tissues.
DR Genevisible; Q8VIG2; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; ISO:RGD.
DR GO; GO:1905762; F:CCR4-NOT complex binding; IBA:GO_Central.
DR GO; GO:1903231; F:mRNA base-pairing post-transcriptional repressor activity; IBA:GO_Central.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR GO; GO:0006302; P:double-strand break repair; ISO:RGD.
DR GO; GO:0007143; P:female meiotic nuclear division; ISS:UniProtKB.
DR GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR GO; GO:0016441; P:post-transcriptional gene silencing; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; ISS:UniProtKB.
DR CDD; cd12255; RRM1_LKAP; 1.
DR CDD; cd12256; RRM2_LKAP; 1.
DR Gene3D; 3.30.420.610; -; 6.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR041966; LOTUS-like.
DR InterPro; IPR024768; Marf1.
DR InterPro; IPR045602; MARF1_LOTUS.
DR InterPro; IPR034189; MARF1_RRM1.
DR InterPro; IPR034191; MARF1_RRM2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR021139; NYN_MARF1.
DR InterPro; IPR025605; OST-HTH/LOTUS_dom.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR14379; PTHR14379; 1.
DR Pfam; PF11608; Limkain-b1; 1.
DR Pfam; PF19687; MARF1_LOTUS; 1.
DR Pfam; PF01936; NYN; 1.
DR Pfam; PF12872; OST-HTH; 6.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS51644; HTH_OST; 8.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Differentiation; Meiosis; Oogenesis; Peroxisome; Phosphoprotein;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..1735
FT /note="Meiosis regulator and mRNA stability factor 1"
FT /id="PRO_0000276848"
FT DOMAIN 351..488
FT /note="NYN"
FT DOMAIN 788..867
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 872..946
FT /note="HTH OST-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1000..1076
FT /note="HTH OST-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1096..1170
FT /note="HTH OST-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1172..1248
FT /note="HTH OST-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1256..1331
FT /note="HTH OST-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1332..1407
FT /note="HTH OST-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1408..1482
FT /note="HTH OST-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT DOMAIN 1483..1557
FT /note="HTH OST-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00975"
FT REGION 617..639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 657..721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1671..1718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 664..693
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 701..716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4F3"
FT MOD_RES 757
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4F3"
FT MOD_RES 1088
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4F3"
FT MOD_RES 1090
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4F3"
FT MOD_RES 1568
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4F3"
FT CONFLICT 1184
FT /note="L -> F (in Ref. 1; BAB82432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1735 AA; 192642 MW; 9A1999814518EF4C CRC64;
MEGNGTENPC SRTLGWLHQN NDAKPWLWKF SGCFSLPEQT LPLSSQTKEY MENKKAAVEL
KDIPSPLHVG SKFFPAVPLP DIHSLQQPKV QLSAIPKVSC CAHCPNEPST SPMPFGGGSG
SSGRSGSLIH TGSLLDSPST GTVTCQVGSD FAFQSVSSLQ NASARNNLVG LASDLPSMCV
DSNLPSCKHL SCCGKFHFQS CRSNVHKLHQ FQTLQGCTSA GYFPCSDFPS EAPGHLEEHL
SHSELTPHLC TNSLHLNVAP PVCLKGSLYC EDCLNKPARN SIIDAAKIWP NIPPPSTQPA
PRAVPLCNRC GTKGMEKETS LLATSLGKAA SKFGSPEVAV TGQVLETLPP IGVFWDIENC
SVPSGRSATT VVQRIREKFF RGHREAEFIC VCDISKENKE VIQELNNCQV TVAHINATAK
NAADDKLRQS LRRFANTHTA PATVVLVSTD VNFALELSDL RHRHGFHIIL VHKNQASEAL
LHHANQLIRF EEFISDLPPR LPLKIPCHTL LYVYNLPANK DGKSISNRLR RLSDNCGGKV
LSITGCSAIL RFINQDSAER AQKRMENEDV FGNRIIVSFT PKHRECFEAK SSNAVVDKAK
SPKKVKNTKL CLIKDTGEQS PSAKAMPGKG LQANSGSATK NTNFKGLQEL CRMEPKSGNR
IRDHQQGHGS LATLPNSGPT ASVPTLKNTG VRETLHRSSQ KKENLSSQSI ASSPAQKKKR
EGTVFQISYP SAFSQLIASR QVSPLFSSQS WSPRSMSPNL LNTASPLAFN IANPSSAADC
SDPFANGVDI QVSNVDYRLS RKELQQLLQE AFSKHGQVKS VELSPHTDYQ LKAIVQMRNL
HDAICAVNSL HRYKIGSKKI LVSLSTGAAN KSLSLLSTET MSILQDAPAC CLPLFKFIDI
YEKKYGHKLN VSDLYKLTDM IAIREQGNGR LVCLLPSNQA RQSPLGSSQS HDGSSTNCSP
VLFEELEYHE SVCKQHCSNK DFSELVFDPD SYKVPFVVLS LKVFAPQVHS LLQTHEGTVP
LLSFPDCYAA EFGDLEITQD RDKGVPLEHF ITCIPGVNIA TAQNGVKVVK WIHNKPPPPN
TDPWLLRSKS PVRNPQLIQF SREVIDLLKN QPSCILPISN FIPLYHHHFG KQCRVSDYGY
SKLIELLEAV PHVLQILGMG SKRLLTLTHR AQVKRFTQDL LKLLKSQASK QVIVSDFSQA
YHWCFSKDWD VTEYGVCDLI DIISEIPDTT ICLSQQDDDM VICIRKRERT QDEIERTKQF
SKDVVDLLRH QPHFRMPFNK FIPSYHHHFG RQCKLAYYGF TKLLELLEAI PEILQVLECG
EEKILTLTEV ERFKALAAQF VKLLRSQKGN CLMMTDLFTE YAKTFGYTFR LQDYDVSSVS
ALTQKLCHVV KVADMESGKQ IQLITRKSLR PLTVQLLVVL MSWEGDAYLP VDELKRHYET
THSTPLNPCE YGFMTLTELL KSLPSLVEVF MNDKAEECVK LTSLYLFAKN VRSLLHTYHY
QQIFLHEFAM AYTKYVGETL QPKTYGYSSV EELLGAIPQV VWIKGHGHKR IVVLKNDMKN
RLSSFDLSPV NHEDQPAVSR QILEVPEPHL ASELHLRTGP SQMEQELLQL ANSSPVDLLC
APVPSCLPSP QLRPDPVILQ PTDLIQFEEH PQEPLGVLFL NQEEKTEIPI PVKKGNLSCN
SSPSSPASAS APPKPSSEAP GLLISKDAVE SPAKKQPKNR VKLAANFSFA PVTKL
