ID   MAS11_AGRRH             Reviewed;         430 AA.
AC   P27874;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Agropine synthesis reductase;
DE            EC=1.-.-.-;
GN   Name=mas1;
OS   Agrobacterium rhizogenes.
OG   Plasmid pRiA4b.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A4;
RX   PubMed=1852015; DOI=10.1016/0147-619x(91)90004-g;
RA   Bouchez D., Tourneur J.;
RT   "Organization of the agropine synthesis region of the T-DNA of the Ri
RT   plasmid from Agrobacterium rhizogenes.";
RL   Plasmid 25:27-39(1991).
CC   -!- FUNCTION: Reduces deoxy-fructosyl-glutamine to mannopine.
CC   -!- PATHWAY: Opine metabolism; mannopine biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; X51338; CAA35718.1; -; Genomic_DNA.
DR   PIR; JQ1050; JQ1050.
DR   AlphaFoldDB; P27874; -.
DR   SMR; P27874; -.
DR   STRING; 359.CN09_18925; -.
DR   eggNOG; COG1028; Bacteria.
DR   UniPathway; UPA00736; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   Gene3D; 3.40.50.1240; -; 1.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR015845; Mas1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   PIRSF; PIRSF036951; Mas1; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SMART; SM00855; PGAM; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53254; SSF53254; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..430
FT                   /note="Agropine synthesis reductase"
FT                   /id="PRO_0000054722"
FT   ACT_SITE        346
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         203..227
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   430 AA;  47722 MW;  A227E6481BFE1602 CRC64;
     MPLPRAYLGT AQPPASVKEF YFVRHGATDL NEKEMHLQGE KHWGVQGAGT NIGLNGTGKR
     QAVLAGNVLR KLPIGSVVCS PLLRAIQTAL IANIGFLCFD IDEDLKERDF GKHEGGYGPL
     KMFEDNYPDC EDTEMFSLRV AKALTHAKNE NTLFVSHGGV LRVIAALLGV DLTKEHTNNG
     RVLHFRRGFS HWTVEIHQSP VILVSGSNRG VGKAIAEDLI AHGYRLSLGA RKVKDLEVAF
     GPQDEWLHYA RFDAEDHGTM AAWVTAAVEK FGRIDGLVNN AGYGEPVNLD KHVDYQRFHL
     QWYINCVAPL RMTELCLPHL YETGSGRIVN INSMSGQRVL NPLVGYNMTK HALGGLTKTT
     QHVGWDRRCA AIDICLGFVA TDMSAWTDLI ASKDMIQPED IAKLVREAIE RPNRAYVPRS
     EVMCIKEATR