ID   MAS12_AGRRH             Reviewed;         430 AA.
AC   P50201;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Agropine synthesis reductase;
DE            EC=1.-.-.-;
GN   Name=mas1;
OS   Agrobacterium rhizogenes.
OG   Plasmid pRi8196.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1909028; DOI=10.1073/pnas.88.17.7763;
RA   Hansen G., Larribe M., Vaubert D., Tempe J., Biermann B.J., Montoya A.L.,
RA   Chilton M.D., Brevet J.;
RT   "Agrobacterium rhizogenes pRi8196 T-DNA: mapping and DNA sequence of
RT   functions involved in mannopine synthesis and hairy root differentiation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:7763-7767(1991).
CC   -!- FUNCTION: Reduces deoxy-fructosyl-glutamine to mannopine.
CC   -!- PATHWAY: Opine metabolism; mannopine biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; M60490; AAA22101.1; -; Genomic_DNA.
DR   PIR; I39727; I39727.
DR   RefSeq; WP_052365245.1; NZ_SGOI01000012.1.
DR   AlphaFoldDB; P50201; -.
DR   SMR; P50201; -.
DR   UniPathway; UPA00736; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   Gene3D; 3.40.50.1240; -; 1.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR015845; Mas1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   PIRSF; PIRSF036951; Mas1; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SMART; SM00855; PGAM; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53254; SSF53254; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..430
FT                   /note="Agropine synthesis reductase"
FT                   /id="PRO_0000054723"
FT   ACT_SITE        346
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         203..227
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   430 AA;  47583 MW;  DD1B7E8D92854D2C CRC64;
     MPISLEYLGP AQAPASVGDF YFLRHGKTDL NEKEVFVQGE KHWGVQGAGT NIGLNETGKR
     QAVLAGNVLR KLPISSVVCS PLLRAIQTAV IANIGCLCFE IDDDLKERDF GKHEGGYGPL
     KMFEENYPDT ENTELFSMRV AKALAHAETE NTLFVSHGGV LRVVAALLGV ELTKEHTDNG
     RVLHFRRGCP QWTVEIHQSP VILISGPSRG IGKAIAENLI AHGYRMSLGA RRVKDLESAF
     GPQSEWLHYA RFDAEDNDTM AAWVTAAVEK FGRIDGLINN AGCGEPVNLE KDIDYKQFHR
     QWHINCVAPL RMTELCLPYL CETGSGRVVN INSMSGQRVL NPFVGYNMTK HALGGLTKTT
     QHVGWDRGVR AIDICPGFVA TEMSAWTDLI SSNDMIQPND IANLVREAIE RPNRAYVPRS
     EVMCMKESIH