ID   MAS1_AGRT9              Reviewed;         430 AA.
AC   P50202;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Agropine synthesis reductase;
DE            EC=1.-.-.-;
GN   Name=mas1;
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   AGRICOLA=IND84096335; DOI=10.1007/BF01578595;
RA   Barker R.F., Idler K.B., Thompson D.V., Kemp J.D.;
RT   "Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens
RT   octopine Ti plasmid pTi15955.";
RL   Plant Mol. Biol. 2:335-350(1983).
CC   -!- FUNCTION: Reduces deoxy-fructosyl-glutamine to mannopine.
CC   -!- PATHWAY: Opine metabolism; mannopine biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; X00493; CAA25187.1; -; Genomic_DNA.
DR   PIR; S28707; S28707.
DR   RefSeq; NP_059684.1; NC_002377.1.
DR   AlphaFoldDB; P50202; -.
DR   SMR; P50202; -.
DR   UniPathway; UPA00736; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   Gene3D; 3.40.50.1240; -; 1.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR015845; Mas1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   PIRSF; PIRSF036951; Mas1; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SMART; SM00855; PGAM; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53254; SSF53254; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..430
FT                   /note="Agropine synthesis reductase"
FT                   /id="PRO_0000054724"
FT   ACT_SITE        346
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         203..227
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   430 AA;  46751 MW;  CE47F536BDB92BAD CRC64;
     MPISQTYLGS AKAPAGIKSF YFIRHGATDL NEKEIVVNGE KLWGVQGSGT NIGLNAKGER
     QALLAGNVLR SLPISGVVCS PLLRALQTAF IANPGCPSFQ IANDLQERDF GTHEGGFGPL
     QMFEDDYPDC ESTEIFSIHV AKALKHACRE NVLLVAHGGV LRVVAALLGV AITDEHTANG
     RVLHFSVVAD NWSVRVIQSP VVMVSGVTRG IGKAIAEDLI RHGYRVSLGA RNIQDLVAAF
     GDENEALHYA RFDALDHSSM KDWVDTTIAK FNRIDGLVNN AGCGDHVDLE KEINVELLQK
     QWDINCVAPL IMTKLCMPYL IESGSGRIVN LNSMSGQRVA NSLVGYNMTK HGLAGLTKTT
     QHVGWDHGVR AVDICPGFVA TNMSSWTNLI GPDEMIQPED IAKLVRAAME RPNRAFVPKN
     EVLCMKESTR