ID   MASOD_ORADR             Reviewed;          79 AA.
AC   P86146; C7DT07;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Eumenine mastoparan-OD {ECO:0000303|PubMed:18695936};
DE            Short=EMP-OD {ECO:0000303|PubMed:18695936};
DE   AltName: Full=Venom peptide 1 {ECO:0000305|PubMed:19857508, ECO:0000312|EMBL:ACU30738.1};
DE            Short=OdVP1 {ECO:0000303|PubMed:19857508};
DE            Short=VP1 {ECO:0000303|PubMed:19857508};
DE   Flags: Precursor;
GN   Name=VP1;
OS   Orancistrocerus drewseni (Solitary wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Eumeninae; Orancistrocerus.
OX   NCBI_TaxID=529024;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 63-76, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT LEU-76.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=19857508; DOI=10.1016/j.toxicon.2009.10.023;
RA   Baek J.H., Lee S.H.;
RT   "Isolation and molecular cloning of venom peptides from Orancistrocerus
RT   drewseni (Hymenoptera: Eumenidae).";
RL   Toxicon 55:711-718(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 63-76, FUNCTION, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-76.
RC   TISSUE=Venom;
RX   PubMed=18695936; DOI=10.1007/s00726-008-0166-y;
RA   Murata K., Shinada T., Ohfune Y., Hisada M., Yasuda A., Naoki H.,
RA   Nakajima T.;
RT   "Novel mastoparan and protonectin analogs isolated from a solitary wasp,
RT   Orancistrocerus drewseni drewseni.";
RL   Amino Acids 37:389-394(2009).
RN   [3]
RP   REVIEW.
RX   PubMed=27096870; DOI=10.3390/toxins8040114;
RA   Konno K., Kazuma K., Nihei K.;
RT   "Peptide toxins in solitary wasp venoms.";
RL   Toxins 8:114-114(2016).
CC   -!- FUNCTION: Has potent hemolytic activity against ovine erythrocytes at
CC       micromolar concentrations. Also exhibits strong antimicrobial activity
CC       against the fungi C.albicans and B.cinerea, and weaker activity against
CC       the Gram-negative bacterium E.coli and Gram-positive bacterium
CC       S.aureus. {ECO:0000269|PubMed:18695936, ECO:0000269|PubMed:19857508}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18695936,
CC       ECO:0000269|PubMed:19857508}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:18695936, ECO:0000305|PubMed:19857508}.
CC   -!- MASS SPECTROMETRY: Mass=1552.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18695936};
CC   -!- MASS SPECTROMETRY: Mass=1551.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18695936};
CC   -!- MASS SPECTROMETRY: Mass=1551.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19857508};
CC   -!- SIMILARITY: Belongs to the MCD family. Mastoparan subfamily.
CC       {ECO:0000269|PubMed:18695936}.
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DR   EMBL; GQ205580; ACU30738.1; -; mRNA.
DR   AlphaFoldDB; P86146; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Fungicide; Hemolysis; Secreted; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..62
FT                   /evidence="ECO:0000305|PubMed:18695936,
FT                   ECO:0000305|PubMed:19857508"
FT                   /id="PRO_0000390690"
FT   PEPTIDE         63..76
FT                   /note="Eumenine mastoparan-OD"
FT                   /evidence="ECO:0000269|PubMed:18695936,
FT                   ECO:0000269|PubMed:19857508"
FT                   /id="PRO_0000372683"
FT   MOD_RES         76
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:18695936,
FT                   ECO:0000269|PubMed:19857508"
SQ   SEQUENCE   79 AA;  8029 MW;  BF6291638F3675F6 CRC64;
     MKQTIVIVLL AAVAMMACLQ MVAAEPLPEA APAPSPLAEA EALASPIAEA LANPEALASP
     EAGRILSFIK GLAEHLGKK