ID   MAST_VESXA              Reviewed;          14 AA.
AC   P01515;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   02-JUN-2021, entry version 71.
DE   RecName: Full=Mastoparan-X;
DE            Short=MP-X;
OS   Vespa xanthoptera (Japanese yellow hornet) (Vespa simillima xanthoptera).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Vespinae; Vespa.
OX   NCBI_TaxID=7448;
RN   [1]
RP   PROTEIN SEQUENCE, AND AMIDATION AT LEU-14.
RC   TISSUE=Venom;
RX   PubMed=540363; DOI=10.1248/cpb.27.1945;
RA   Hirai Y., Kuwada M., Yasuhara T., Yoshida H., Nakajima T.;
RT   "A new mast cell degranulating peptide homologous to mastoparan in the
RT   venom of Japanese hornet (Vespa xanthoptera).";
RL   Chem. Pharm. Bull. 27:1945-1946(1979).
RN   [2]
RP   STRUCTURE BY NMR.
RX   PubMed=1610813; DOI=10.1021/bi00139a032;
RA   Wakamatsu K., Okada A., Miyazawa T., Ohya M., Higashijima T.;
RT   "Membrane-bound conformation of mastoparan-X, a G-protein-activating
RT   peptide.";
RL   Biochemistry 31:5654-5660(1992).
RN   [3]
RP   STRUCTURE BY NMR.
RX   PubMed=9537994; DOI=10.1021/bi972756p;
RA   Kusunoki H., Wakamatsu K., Sato K., Miyazawa T., Kohno T.;
RT   "G protein-bound conformation of mastoparan-X: heteronuclear
RT   multidimensional transferred nuclear overhauser effect analysis of peptide
RT   uniformly enriched with 13C and 15N.";
RL   Biochemistry 37:4782-4790(1998).
CC   -!- FUNCTION: Mast cell degranulating peptide. Activates G proteins that
CC       couple to phospholipase C.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the MCD family. Mastoparan subfamily.
CC       {ECO:0000305}.
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DR   PIR; A01778; QMVHXX.
DR   PDB; 1A13; NMR; -; A=1-14.
DR   PDB; 2CZP; NMR; -; A=1-14.
DR   PDBsum; 1A13; -.
DR   PDBsum; 2CZP; -.
DR   BMRB; P01515; -.
DR   SMR; P01515; -.
DR   TCDB; 1.C.32.1.2; the amphipathic peptide mastoparan (mastoparan) family.
DR   PRIDE; P01515; -.
DR   EvolutionaryTrace; P01515; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043303; P:mast cell degranulation; IEA:UniProtKB-KW.
DR   IDEAL; IID50241; -.
DR   InterPro; IPR013213; Mastoparan.
DR   Pfam; PF08249; Mastoparan; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amidation; Direct protein sequencing;
KW   Mast cell degranulation; Secreted.
FT   PEPTIDE         1..14
FT                   /note="Mastoparan-X"
FT                   /id="PRO_0000044063"
FT   MOD_RES         14
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:540363"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:1A13"
FT   HELIX           6..10
FT                   /evidence="ECO:0007829|PDB:1A13"
SQ   SEQUENCE   14 AA;  1557 MW;  C85DE0D7AA7AB0DD CRC64;
     INWKGIAAMA KKLL