ID   MASY_BRANA              Reviewed;         561 AA.
AC   P13244; O04911;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            EC=2.3.3.9;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2914930; DOI=10.1016/s0021-9258(19)81681-2;
RA   Comai L., Baden C.S., Harada J.J.;
RT   "Deduced sequence of a malate synthase polypeptide encoded by a subclass of
RT   the gene family.";
RL   J. Biol. Chem. 264:2778-2782(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Global; TISSUE=Cotyledon;
RA   Olesen C., Thomsen K.K., Svendsen I., Brandt A.B.;
RL   Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; J04468; AAA32996.1; -; mRNA.
DR   EMBL; Y13357; CAA73793.1; -; mRNA.
DR   PIR; A31428; SYRPMA.
DR   RefSeq; XP_013720273.1; XM_013864819.1.
DR   RefSeq; XP_013721514.1; XM_013866060.1.
DR   AlphaFoldDB; P13244; -.
DR   SMR; P13244; -.
DR   EnsemblPlants; CDY04901; CDY04901; GSBRNA2T00119622001.
DR   GeneID; 106424075; -.
DR   Gramene; CDY04901; CDY04901; GSBRNA2T00119622001.
DR   KEGG; bna:106424075; -.
DR   OMA; CHKRGAP; -.
DR   UniPathway; UPA00703; UER00720.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Peroxisome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..561
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166866"
FT   MOTIF           559..561
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        177
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        462
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        29
FT                   /note="Q -> L (in Ref. 2; CAA73793)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        163
FT                   /note="K -> I (in Ref. 2; CAA73793)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        172
FT                   /note="A -> V (in Ref. 2; CAA73793)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        175
FT                   /note="F -> W (in Ref. 2; CAA73793)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   561 AA;  63728 MW;  215A1027CD991AA3 CRC64;
     MELETSVYRP NVAVYDSPDG VEVRGRYDQV FAKILTRDAL GFVAELQREF RGHVRYAMEC
     RREVKRRYNS GAVPGFDPST KFIRDGEWVC ASVPPAVADR RVEITGPVER KMIINALNSG
     AKVFMADFED ALSPSWENLM RGQVNLKDAV DGSITFNDKA RNKVYKLNDQ VAKLFVRPRG
     WHLPEAHILI DGEPATGCLV DFGLYFFHNY AKFRQTQGSG FGPFFYLPKM EHSREAKIWN
     SVFERAEKMA GIERGSIRAT VLIETLPAVF QMNEILYELR DHSVGLNCGR WDYIFSYVKT
     FQAHPDRLLP DRVLVGMGQH FMRSYSDLLI RTCHKRGVHA MGGMAAQIPI RDDPKANEMA
     LDLVKKDKLR EVRAGHDGTW AAHPGLIPIC MDAFSHMGNN PNQIKSMKRD DASAITEEDL
     LQIPRGVRTL EGLRLNTRVG IQYLAAWLTG SGSVPLYNLM EDAATAEISR VQNWQWIRYG
     VELDGDGLGV RVSKELFGRV VEEEMERIEK EVGKDKFKRG MYKEACKMFT KQCTAAELDD
     FLTLAVYDHI VAHYPINASR L