ID   MASY_DICDI              Reviewed;         543 AA.
AC   Q8T2K9; Q552X6;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 2.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Malate synthase;
DE            EC=2.3.3.9;
GN   Name=masA; ORFNames=DDB_G0275887;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12796308; DOI=10.1128/ec.2.3.627-637.2003;
RA   Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S.,
RA   Morio T., Urushihara H., Tanaka Y., Loomis W.F.;
RT   "Changing patterns of gene expression in Dictyostelium prestalk cell
RT   subtypes recognized by in situ hybridization with genes from microarray
RT   analyses.";
RL   Eukaryot. Cell 2:627-637(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- DEVELOPMENTAL STAGE: Late prestalk cell-specific expression.
CC       {ECO:0000269|PubMed:12796308}.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000013; EAL69694.2; -; Genomic_DNA.
DR   RefSeq; XP_643552.2; XM_638460.2.
DR   AlphaFoldDB; Q8T2K9; -.
DR   SMR; Q8T2K9; -.
DR   STRING; 44689.DDB0230181; -.
DR   PaxDb; Q8T2K9; -.
DR   EnsemblProtists; EAL69694; EAL69694; DDB_G0275887.
DR   GeneID; 8620134; -.
DR   KEGG; ddi:DDB_G0275887; -.
DR   dictyBase; DDB_G0275887; masA.
DR   eggNOG; KOG1261; Eukaryota.
DR   HOGENOM; CLU_018928_3_0_1; -.
DR   InParanoid; Q8T2K9; -.
DR   OMA; CHKRGAP; -.
DR   PhylomeDB; Q8T2K9; -.
DR   UniPathway; UPA00703; UER00720.
DR   PRO; PR:Q8T2K9; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005782; C:peroxisomal matrix; IBA:GO_Central.
DR   GO; GO:0004474; F:malate synthase activity; IBA:GO_Central.
DR   GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 1.20.1220.12; -; 1.
DR   Gene3D; 3.20.20.360; -; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   PANTHER; PTHR42902; PTHR42902; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; SSF51645; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Reference proteome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN           1..543
FT                   /note="Malate synthase"
FT                   /id="PRO_0000365606"
FT   ACT_SITE        162
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        449
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   543 AA;  62030 MW;  6F8E37DCCC9904B2 CRC64;
     MLRGIEIRGK VDKNVKSILT EECLTFLGEL ERRFGGIRKN LLQKRLDRQI DINNGILPSF
     LKDSDCKRAT DKNWKCSSVP LEIQDRRVEI TGPTDRKMVI NALNSGAKVF MADFEDANCP
     NWENSIHGQQ NMIDANNRTI SFTSAEGRKY ELNKQVAVLF VRPRGWHLNE DHLAIDGLSM
     SGSLFDFGCY IFHNHQILKN RNSNPYFYLP KMESHLEARL WNDVFVFSQN YLGMPIGTIK
     ATVLIETILA SFEMDEILYE LRDHSAGLNC GRWDYIFSFI KKFQSYPDKM LPDRAKVTMT
     SPFMDSYVKL LIYTCHKRGV HAMGGMAAQI PIKNNEQANN AAMEKVRLDK QREVRAGHDG
     TWVAHPALIP IAMEQFNTHM KSQNQISYIP SSTAPNFEEI AKSLLSVDPV KPGDITEDGF
     RSNIVVGILY LEAWLNGNGC VPIHNLMEDA ATAEISRSQI WQWIKHKAPL PDGKSFVTLQ
     YYNRIFKEES DKLEKAHPNS KTLTDAIQIF NNLIVSPNFI DFLTPSCYKF VVDSERKTFS
     PKL